FV3000
Specifications
| FV3000 | FV3000RS | ||
|---|---|---|---|
| Main Laser Combiner | Visible Light Laser |
405 nm: 50 mW, 488 nm: 20 mW, 561 nm: 20 mW, 640 nm: 40 mW
One optional laser port for the sub laser combiner or optional laser unit | |
| Optional Laser | Sub Laser Combiner |
Maximum 3 laser units as follows:
445 nm: 75 mW, 514 nm: 40 mW, 594 nm: 20 mW, fiber connected to main laser combiner | |
| Single Laser Unit | 445 nm: 75 mW, 514 nm: 40 mW, or 594 nm: 20 mW, directly connected to main laser combiner | ||
| Single NIR Laser Unit | 730 nm: 30 mW, 785 nm: 100 mW, connected to scanner via optional port | ||
| Scanner | Scanning Method | 2 silver-coated galvanometer scanning mirrors |
2 silver-coated galvanometer scanning mirrors
1 silver-coated resonant and 1 silver-coated galvanometer scanning mirrors |
|
Galvanometer Scanner
(Normal Imaging) |
Scanning Resolution: 64 × 64 to 4096 × 4096 pixels
Scanning Speed (One Way): 512 × 512 with 1.1 s–264 s. pixel time: 2 µs–1000 µs Scanning Speed (Round Trip): 512 × 512 with 63 ms–250 ms, 256 × 256 with 16 ms–125 ms Optical Zoom: 1X–50X in 0.01X increments Scan Rotation: Free rotation (360 degree) in steps of 0.1 degree Scanning Mode: PT, XT, XZ, XY, XZT, XYT, XYZ, XYλ, XYZT, XYλT, XYλZ, XYλZT ROI scanning, rectangle clip, ellipse, polygon, free area, line, free line and point, tornado mode only for stimulation | ||
|
Resonant Scanner
(High-Speed Imaging) | - |
Scanning Resolution: 512 × 32 to 512 × 512 pixels
Scanning Speed: 30 fps at 512 × 512, 438 fps at 512 x 32 Optical Zoom: 1X–8X in 0.01X increments Scanning Mode: XT, XZ, XY, XZT, XYT, XYZ, XYλ, XYZT, XYλT, XYZ, XYλZT ROI scanning, rectangle clip, line | |
| Pinhole | Single motorized pinhole, pinhole diameter ø50–800 µm (1 µm steps) | ||
| Field Number (FN) | 18 | ||
| Dichromatic Mirror Turret | 8 positions (high-performance DMs and 10/90 mirror) | ||
| Optional Unit for Scanner | Laser power monitor, optional laser port | ||
| Spectral Detector | Detector Module | Cooled GaAsP photomultiplier (high-sensitivity type) or Multi-Alkali photomultiplier, 2 channels | |
| Spectral Method |
Motorized volume phase holographic transmission diffraction grating, motorized adjustable slit,
selectable wavelength bandwidth: 1–100 nm, wavelength resolution: 2 nm | ||
| Dichromatic Mirror Turret | 8 positions (high-performance DMs and mirror) | ||
| NIR Detector | Detector Module | GaAs photomultiplier tube, 1 channel or 2 channels with filter cube | |
| Fluorescence Illumination Unit | External fluorescence light source, fiber adaptor to the optical port of the scan unit, motorized switching between the LSM light path and fluorescence illumination | ||
| Transmitted Light Detector Unit | Module with integrated external transmitted light photomultiplier detector and LED lamp, motorized switching | ||
Microscope |
| Inverted frame | Upright frame (for imaging) | Upright frame (for electrophysiology) | |
|---|---|---|---|
| Microscope Frame |
Motorized inverted microscope
IX83 (IX83P2ZF) |
Motorized fixed stage upright microscope
BX63L |
Motorized fixed stage upright microscope
BX63L |
| Revolving Nosepiece | Motorized sextuple revolving nosepiece | Motorized septuple revolving nosepiece |
Coded swing nosepiece
Coded slider nosepiece |
| Condenser | Motorized long working distance condenser | Motorized universal condenser | Manual long working distance condenser |
| Focus Stroke |
Built-in motorized nosepiece focus
Stroke: minimum increment: 0.01 μm | ||
Software |
| Basic Features |
GUI designed for darkroom environment. User-arrangeable layout.
Acquisition parameter reload features. Hard disk recording capability; adjust laser power and HV with Z-stack acquisition. Z-stack with alpha blending, maximum-intensity projection, iso-surface rendering. |
|---|---|
| 2D Image Display | Each image display: single-channel side-by-side, merge, cropping, live tiling, live tile, series (Z/T/λ), LUT: individual color setting, pseudo-color, comment: graphic and text input. |
| 3D Visualization and Observation |
Interactive volume rendering: volume rendering display, projection display, animation displayed.
3D animation (maximum intensity projection method, α blending) 3D and 2D sequential operation function. |
| Image Format |
OIR image format
8/16-bit gray scale/index color, 24/ 32/ 48-bit color, JPEG/ BMP/ TIFF image functions, Olympus multi-tif format. |
| Spectral Unmixing | Fluorescence spectral unmixing modes (up to 16 channels) |
| Image Analysis | Region and line measurements, intensity plot over time/Z, colocalization analysis. |
| Statistical Processing | 2D data histogram display. |
| Optional Software | Motorized-stage control |
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