The Inverted Microscope System for Routine Work
The IX53 has been designed from the ground up to be the finest microscope available for routine inverted microscopic analysis. The combination of excellent optical performance and mechanical quality results in a microscope system of outstanding value and comfort for regular use. Features such as the pre-centered phase contrast, relief contrast and the flexible UIS2 DIC optics enable easy visualization across all magnifications for both thin and thick specimens. Available condensers with different working distances and long working objectives allow observation and documentation of even the most complex samples.
Not Available in Your Country
Sorry, this content is not
available in your country.
The IX53 system is the ideal solution for routine analysis. End-users can improve research efficiency by recording cell dynamics across a wide area. Reliability can also be enhanced through accurate position and light reproducibility.
IX53: One-deck System
An outstanding, cost-effective microscope for brightfield and fluorescence applications.
Olympus UIS2 infinity-corrected optics ensure high optical transmittance with a broad range of objectives. UIS2 optics feature wide chromatic correction and enable high resolution, high S/N primary images regardless of the observation method. The wide field of view and Fly-Eye lens system provide uniform fluorescence images and enable the use of sCMOS cameras with large sensors.
Apochromatic Objectives Enable High-Resolution Phase Contrast and Fluorescence Observation
Phase contrast apochromatic objectives (UPLSAPO100XOPH, PLAPON60XOPH) enable high-precision imaging free from image shift, even during simultaneous phase contrast and fluorescence observation. This eliminates the need to change objectives when switching observation methods.
Silicone Objectives* Enable High-Resolution Observation Deep into Live Cells
Olympus offers three high-NA silicone immersion objectives:UPLSAPO30XS, UPLSAPO40XS, and UPLSAPO60XS. The refractive index of silicone oil (Refractive index: ne≈1.40) is close to that of living tissue (Refractive index: ne≈1.38), enabling high-resolution observation deep inside living tissue with minimal spherical aberration caused by refractive index mismatch. Silicone oil does not dry out or harden, so there is never a need to readminister the oil, making it ideal for extended time-lapse observations.
*Use dedicated silicone oil.
Special Objective Available for iPS/ES and Floating Cell Observation
This high-NA phase contrast objective (UCPLFLN20XPH) is especially suited for observation using plastic dishes. It enables high-resolution observation of the cell proliferation process and delivers improved contrast across a wide area.
High S/N Fluorescence Mirror Units for Efficient Signal Detection
All fluorescence mirror units feature filters treated with a specially-developed coating that minimizes noise by absorbing more than 99 % of stray light. The outstanding performance and high transmittance of the mirror units ensure efficient fluorescence signal detection.
Smooth Tracking at High Magnification
The IX3-SVR manual stage features a smooth positioning system which enables the easy tracking of cells, even at high magnifications. The observation position limits can be set by the user to immobilize the stage, ensuring that the observation position is maintained during operations such as reagent application, even if the stage is inadvertently touched. It is also possible to remove 35 mm dishes from the stage, place them in an incubator and return them to the exactly location previously
This feature was based on the technical development at the RIKEN BSI-Olympus Collaboration Center.
Easily Achieve Koehler Illumination
The condenser can be moved and easily reset to Koehler Illumination using the conveniently-located condenser lock and control knobs.
Long Life 130W Mercury Lamp (U-HGLGPS)
The U-HGLGPS fluorescence light source delivers bright, even illumination and features a long life, 2,000-hour mercury burner (average lifetime). The unit is maintenance-free and does not require centering adjustment. A liquid light guide prevents heat transfer to samples, thus allowing extended observations to be carried out without concern.
|Observation Method > Fluorescence (Blue/Green Excitations)||✓|
|Observation Method > Fluorescence (Ultraviolet Excitations)||✓|
|Observation Method > Differential Interference Contrast||✓|
|Observation Method > Phase Contrast||✓|
|Observation Method > Relief Contrast||✓|
|Observation Method > Simple Polarized Light||✓|
|Observation Method > Brightfield||✓|
|Zoom > Motorized||No|
|Illuminator > Transmitted Koehler Illuminator > LED Lamp|
|Illuminator > Transmitted Koehler Illuminator > Halogen Lamp|
|Illuminator > Fluorescence Illuminator > Hg Lamp|
|Illuminator > Fluorescence Illuminator > Xenon Lamp|
|Illuminator > Fluorescence Illuminator > Light Guide Illumination|
|Revolving Nosepiece > Manual > Standard Type||Built-in 6 position|
|Stage > Mechanical > Plain Stage||✓|
|Stage > Mechanical > IX3-SVR Mechanical Stage with Right Handle|
|Stage > Mechanical > IX2-GS Gliding Stage||✓|
|Condenser > Manual > Long Working Distance Universal Condenser||NA 0.55/ W.D. 27 mm|
|Condenser > Manual > Mid Long Working Distance Condenser||NA 0.5/ W.D. 45 mm|
|Condenser > Manual > Ultra Long Working Distance Condenser||NA 0.3/ W.D. 73.3 mm|
|Observation Tubes > Widefield (FN 22) > Binocular||✓|
|Observation Tubes > Widefield (FN 22) > Tilting Binocular||✓|
|Dimensions||323 (W) x 475 (D) x 657 (H) mm|
|Operating Environment > Indoor Use > Ambient Temperature||5 - 40 ºC (41 - 104 ºF)|
|Operating Environment > Indoor Use > Maximum Relative Humidity||80 % for temperatures up to 31 ºC (88 ºF), decreasing linearly through 70 % at 34 ºC (93 ºF), 60 % at 37 ºC (99 ºF), to 50 % relative humidity at 40 ºC (104 ºF)|
|Operating Environment > Indoor Use > Supply Voltage Fluctuations||±10 %|