The Inverted Microscope System for Advanced Live Cell Imaging
The IX73 inverted microscope system sets new standards in advanced live cell imaging with its compact frame, outstanding optical performance and exceptional flexibility. Manual encoded or semi-motorized options enable a variety of component combinations. The IX73 is available as a one-deck system with a low, ergonomic stage or as a two-deck system with additional expansion capabilities. Both provide the ability to perform a multitude of imaging applications, from fast advanced fluorescence imaging and other demanding techniques to routine testing and documentation.
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The semi-motorized IX73 is designed to satisfy a variety of research needs. With two deck options and additional modules for expanded functionality, the IX73 is perfectly suited for a changing research environment.
IX73: Two-deck System
The modular IX73 two-deck system can be combined with coded or motorized units for maximum expandability.
IX73: One-deck System
A microscope designed with an emphasis on efficiency. Ideal for documentation, routine testing and other tasks.
Olympus UIS2 infinity-corrected optics ensure high optical transmittance with a broad range of objectives. UIS2 optics feature wide chromatic correction and enable high resolution, high S/N primary images regardless of the observation method. The wide field of view and Fly-Eye lens system provide uniform fluorescence images and enable the use of sCMOS cameras with large sensors.
Apochromatic Objectives Enable High-Resolution Phase Contrast and Fluorescence Observation
Phase contrast apochromatic objectives (UPLSAPO100XOPH, PLAPON60XOPH) enable high-precision imaging free from image shift, even during simultaneous phase contrast and fluorescence observation. This eliminates the need to change objectives when switching observation methods.
Silicone Objectives* Enable High-Resolution Observation Deep into Live Cells
Olympus offers three high-NA silicone immersion objectives:UPLSAPO30XS, UPLSAPO40XS, and UPLSAPO60XS. The refractive index of silicone oil (Refractive index: ne≈1.40) is close to that of living tissue (Refractive index: ne≈1.38), enabling high-resolution observation deep inside living tissue with minimal spherical aberration caused by refractive index mismatch. Silicone oil does not dry out or harden, so there is never a need to readminister the oil, making it ideal for extended time-lapse observations.
*Use dedicated silicone oil.
Special Objective Available for iPS/ES and Floating Cell Observation
This high-NA phase contrast objective (UCPLFLN20XPH) is especially suited for observation using plastic dishes. It enables high-resolution observation of the cell proliferation process and delivers improved contrast across a wide area.
Bright, Uniform Fluorescence Illumination
The fluorescence illuminator (IX3-RFALFE) incorporates a Fly-Eye lens system to provide even light distribution. This provides bright and even illumination to the entire field, including the periphery of the visual field.
High S/N Fluorescence Mirror Units for Efficient Signal Detection
All fluorescence mirror units feature filters treated with a specially-developed coating that minimizes noise by absorbing more than 99 % of stray light. The outstanding performance and high transmittance of the mirror units ensure efficient fluorescence signal detection.
Switch Observation Methods with a Single Touch
A hand switch is available for the IX73 with the ability to program observation procedures and a range of other functions as touch panel soft keys.
Stored Microscope Configurations (Olympus cellSens)
The system saves microscope configurations alongside image data by incorporating a readout of motorized and coded unit position. With this advanced system, a wide range of settings can be recalled to recreate the desired imaging conditions, thereby creating a highly reproducible and easy-to-use, high-end imaging system.
Smooth Tracking at High Magnification
The IX3-SVR manual stage features a smooth positioning system which enables the easy tracking of cells, even at high magnifications. The observation position limits can be set by the user to immobilize the stage, ensuring that the observation position is maintained during operations such as reagent application, even if the stage is inadvertently touched. It is also possible to remove 35mm dishes from the stage, place them in an incubator and return them to the exactly location previously identified.
Easily Achieve Koehler Illumination
The condenser can be moved and easily reset to Koehler Illumination using the conveniently-located condenser lock and control knobs.
Frame Construction Prevents Optical System Contamination
A catch tray under the nosepiece prevents damage to the microscope frequently caused by contamination through spilled liquids and simplifies maintenance.
A wide range of units are available for the Olympus IX3 microscope system, bringing enhanced usability to everything from casual observation to high-end imaging. Simple cassette-like insertion into the deck makes it easy to mount fluorescence mirror turrets, right side ports, mag changers, epi-illuminators and other units.
The large open frame allows a motorized emission filter wheel to be fitted within the infinity space of the microscope. This eliminates image shift between channels and allows the eyepieces to see what the camera detects.
Motorized Fluorescence Mirror Turret (IX3-RFACA)
A non-click turret that can be fitted with up to 8 mirror units and that delivers smooth, rapid cube rotation. The turret can accommodate both 25mm and 32mm diameter filter units, which can easily be inserted and removed without the need of tools.
Manual Right Side Port with C-mount (IX3-RSPC)
The C-mount-equipped right side ports can be fitted with up to two mirror units, enabling advanced applications such as split imaging.
Coded Intermediate Magnification Changer (IX3-CAS)
Magnification can be easily changed between 1x, 1.6x and 2x by adjusting the lever. Since the system is coded, information on intermediate magnifications is saved with image data.
Motorized Fast Filter Wheels and Shutters
With Olympus filter wheels, the user can easily switch between filters in milliseconds. Olympus shutters operate even faster. The IX73 is capable of controlling up to six filter wheels and four shutters, thus enabling complex, multi-modal imaging.
Application-Specific Fluorescence Illuminators
There is an illuminator best-suited for each application. The L-shaped fluorescence illuminator with the Fly-Eye lens system (IX3-RFALFE) provides bright, even illumination without adjustment. The standard L-shaped fluorescence illuminator (IX3-RFAL) is cost-effective and is equipped with both field and aperture iris diaphragms. The straight fluorescence illuminator (IX3-RFA) is ideal for applications demanding intense excitation light. A wide range of light sources is also available, including counter top and frame-mounted units (mercury and xenon).
Long Life 130W Mercury Lamp (U-HGLGPS)
The U-HGLGPS fluorescence light source delivers bright, even illumination and features a long life, 2,000-hour mercury burner (average lifetime). The unit is maintenance-free and does not require centering adjustment. A liquid light guide prevents heat transfer to samples, thus allowing extended observations to be carried out without concern.
Measuring Excitation Light Intensity for High Reproducibility in Imaging Adapter for Excitation Irradiance Meter/IX3-EXMAD*
Olympus now offers an adapter for a power meter that can directly measure the excitation light intensity per unit surface area of the sample, as well as offering irradiance display software. Displays the measurement results on a monitor and records the data, eliminating The need for laborious calculations. This makes it possible to check the excitation light intensity before starting an experiment, enhancing the reliability of experiments. Data can also be easily shared.
*This equipment was based on the technical development at RIKEN BSI-Olympus Collaboration Center.
A Cost-efficient Way to Upgrade to a Motorized Microscope
A wide range of motorized and encoded units are available, including an 8-position motorized fluorescent mirror turret, an encoded fluorescence mirror turret, a motorized 6-position nosepiece, an encoded 6-position nosepiece, a motorized long working distance universal condenser, filter wheels and shutters.
|Observation Method > Fluorescence (Blue/Green Excitations)||✓|
|Observation Method > Fluorescence (Ultraviolet Excitations)||✓|
|Observation Method > Differential Interference Contrast||✓|
|Observation Method > IR-Differential Interference Contrast||✓|
|Observation Method > Phase Contrast||✓|
|Observation Method > Relief Contrast||✓|
|Observation Method > Simple Polarized Light||✓|
|Observation Method > Brightfield||✓|
|Observation Method > Darkfield||✓|
|Zoom > Motorized||No|
|Illuminator > Transmitted Koehler Illuminator > LED Lamp|
|Illuminator > Transmitted Koehler Illuminator > Halogen Lamp|
|Illuminator > Fluorescence Illuminator > Hg Lamp|
|Illuminator > Fluorescence Illuminator > Xenon Lamp|
|Illuminator > Fluorescence Illuminator > Light Guide Illumination|
|Intermediate Magnification Changer > Manual Terret||✓|
|Revolving Nosepiece > Motorized||6 position|
|Revolving Nosepiece > Manual > Coded||6 position|
|Stage > Mechanical > Plain Stage||✓|
|Stage > Mechanical > IX3-SVR Mechanical Stage with Right Handle|
|Stage > Mechanical > GX-SVR Mechanical Stage for GX|
|Stage > Mechanical > IX2-GS Gliding Stage||✓|
|Stage > Mechanical > GX-SFR Flexible Right Handle Stage||✓|
|Condenser > Motorized > Universal Condenser||NA 0.55/ W.D. 26.2mm|
|Condenser > Manual > Universal Condenser||Dry: NA 0.9/ W.D. 1.5 mm, Oil: NA 1.4/ W.D. 0.63 mm (1.25 X - 100 X)|
|Condenser > Manual > Long Working Distance Universal Condenser||NA 0.55/ W.D. 27 mm|
|Condenser > Manual > Mid Long Working Distance Condenser||NA 0.5/ W.D. 45 mm|
|Condenser > Manual > Ultra Long Working Distance Condenser||NA 0.3/ W.D. 73.3 mm|
|Observation Tubes > Widefield (FN 22) > Binocular||✓|
|Observation Tubes > Widefield (FN 22) > Tilting Binocular||✓|
|Observation Tubes > Widefield (FN 22) > Trinocular||✓|
|Observation Tubes > Widefield (FN 22) > Trinocular for Infrared||✓|
|Dimensions||323 (W) x 475 (D) x 656 (H) mm (1 Deck Standard Configuration)|
|Weight||35 kg (1 Deck Standard Configuration)|
|Operating Environment > Indoor Use > Ambient Temperature||5 - 40 ºC (41 - 104 ºF)|
|Operating Environment > Indoor Use > Maximum Relative Humidity||80 % for temperatures up to 31 ºC (88 ºF), decreasing linearly through 70 % at 34 ºC (93 ºF), 60 % at 37 ºC (99 ºF), to 50 % relative humidity at 40 ºC (104 ºF)|
|Operating Environment > Indoor Use > Supply Voltage Fluctuations||±10 %|