Semi-Motorized Fluorescence Microscope
The entire optical path of the BX53 is designed for optimal fluorescence imaging, using UIS2 components that set new standards in precision and clarity. High-quality mirror cube coatings provide excellent transmission and steep cut-offs, while almost all stray light is eliminated from interior surfaces for the best sensitivity and color separation. Other advanced features further facilitate fluorescence microscopy, including a new fly-eye lens system, which delivers homogenous illumination, and automatic magnification and filter cube read-out, which simplifies the repetition of settings. Additionally, the BX53 semi-motorized fluorescence microscope makes highly efficient imaging possible. Automation enables the imaging of large areas in high magnification and also facilitates multicolor fluorescence.
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The Olympus BX53 microscopes offer even greater application flexibility via a series of semi-motorization and automation options, all of which can be controlled, along with illumination and image capture through the cellSens software. The addition of these components, therefore, provides excellent potential for enhanced experimentation.
The BX3 microscope family offers a wide range of features and optical performance for the research market. With an emphasis on the powerful UIS2 optical system and the proven rigid, Y-shaped frame coupled with easy-to-operate front controls, it offers improved versatility and ergonomic operation. The compact space-efficient frame of the BX53 enables comprehensive microscope work, making it an ideal and versatile microscope for any imaging application, and also the perfect starting point for digital imaging, semi-motorization and fluorescence.
Changing transmitted light observation methods means ensuring correct arrangement of ND filters, polarizers / analyzers and condensers on top of using the right objectives. The 8-position universal condenser even controls the top lens to ensure the full magnification range is available. The condenser guarantees that optical elements such as DIC prisms, phase inserts and polarizers can be selected easily. To ensure that the illuminating light is as closely matched to the objectives as possible, the aperture stop is automatically set based on the NA of the objective in use.
The BX53 is designed for maximum flexibility in placement, with a centrally positioned light intensity dial and a fluorescence illuminator shutter that can be operated with either hand. In addition, the fine focus handles can be attached on either side of the microscope.
The motion sensor detects when a user leaves and automatically turns off the transmitted light lamp after about 30 minutes. The energy-saving switch conserves energy and lamp lifetime.
Higher signal-to-noise ratio (S/N) produces fluorescence images with bright colors and dark background, that’s why Olympus has pushed the boundaries of S/N with improved fluorescence detection capacity. Uniform illumination and detection is achieved through the use of high transmission objectives, mirror units, and the incorporation of a fly-eye lens system.
It is important to ensure that the field of view is illuminated evenly. The nature of fluorescence illumination makes this a difficult task, but Olympus has developed the ultimate solution with its fluorescence illumination concept, which incorporates a fly-eye lens system. As a result of this advance, not only are users assured of flawlessly homogeneous illumination across the entire wavelength spectrum, but they also have the benefit of much simpler burner alignment.
The Olympus 8-position fluorescence illuminator allows greater flexibility for a wide variety of fluorescence specimens with its easily exchangeable mirror units. Observations are accelerated further through the reduced need to replace mirror units for multicolor or FISH applications.
The UIS2 fluorescent mirror cube range is optimized for fluorescence imaging. High-quality coatings used on the mirror cubes provide excellent transmission and steep cut-off slopes, while the interior surfaces eliminate over 99% of stray light, ensuring the highest sensitivity and best color separation. The mirror cubes can be exchanged quickly and easily without tools.
At the heart of any light microscope is the quality of the optical system. Olympus UIS2 optical components have been developed to provide the perfect optical system, setting new standards in precision and clarity. The high NA objectives lenses are optimally corrected for chromatic aberrations and deliver high resolution, with even the faintest of signals being collected. By using carefully selected raw materials for glass, and applying advanced UW multi-coatings technology, Olympus has reduced objective autofluorescence and significantly improved the S/N ratio. UW multi-coatings also yields a flat, high transmission over a wide wavelength range, ensuring high performance in research tasks using different types of fluorochromes.
The motorized universal condenser is designed to reduce back-reflections and autofluorescence by swinging its top lens out, automatically closing its diaphragm to the minimum, and locating the wheel in between two positions in fluorescence imaging.
The ability to reduce auto-fluorescence normally associated with immersion oil makes this product well suited for fluorescence microscopy. Reduced noise (autofluorescence) results in an increased signal-to-noise ratio for better fluorescent observation. Temporal change of autofluorescence is less. Especially useful in quantitative observation of single molecule fluorescence, which is easily affected by noise. Resistance to crystallization allows it to be used over long periods of time. The refraction index is the same as other Olympus products, assuring easy integration with your existing microscopy system.
At the microscope level, biological samples tend not to possess inherent contrast, such as color variations, when using standard brightfield illumination. As a result, a number of different ways of generating contrast have been developed. These can be split into two parts: optical contrast methods and sample contrast methods. Whatever the source of contrast, the Olympus BX3 range and UIS2 optical components perform flawlessly, providing sharp and clear images in any contrast method.
A comfortable position is entirely different from person to person and it is, therefore, essential that the microscope can accommodate each user down to the millimeter. The Olympus line-up of observation tubes provides this capability, enabling inclination angle, tube extension and tube height adjustment and thus fully flexible three-dimensional adjustment.
Creating the perfect upright and comfortable posture during microscope operation is not only essential, but entirely dependent on the user. With the ergonomically perfected telescopic, tilting, lifting observation tube, the inclination angle, tube extension and tube height can be adjusted, allowing a fully flexible set-up in three dimensions. As a result the microscope can be adjusted to precisely match the user.
A diverse range of tilting binocular tubes is available meeting various demands. One model generates the conventional inverted observation image, whereas the other produces an erect observation image moving in the same direction as the specimen; this makes it easier to find a specific area in the specimen.
As with quality, true flexibility is a trait that must be designed into an instrument at every stage. With this very goal, Olympus developed the BX3 range of upright research microscopes. The BX53 is the ultimate expression of imaging system versatility; offering extensive choice with numerous optical, semi-motorization and software options, along with ongoing adaptability during use.
Each user has unique posture and positional requirements and, therefore, as well as enabling personalized work areas, the ability to adjust microscope controls aids in providing an ergonomic environment suitable for long-term microscope use without leading to positional or repetitive strain injuries. For the greatest system flexibility and user comfort, the tilting, ergonomic trinocular tube provides excellent eyepiece height adjustment as well as interpupillary distance control, and the optical path slider can be attached to either side of the tube, putting the user in full control of the microscope and ensuring that the microscope adjusts to their posture.
A remote exposure knob can be mounted on either side of the microscope to allow image acquisition at the touch of a button without requiring the user to turn away from the microscope to look at the monitor and use the mouse. This is not only more efficient, but is also ergonomically better for the user.
The BX3 range features optional manual nosepiece and mirror turret modules, which are encoded, enabling users to automatically record and share microscope magnification and setting information for facilitating post imaging treatments. This readout provides the correct metadata automatically to the Olympus cellSens software packages ensuring no mistakes or scaling errors are made when documenting images.
Research microscopy is about more than just the microscope of the imaging capability because each investigation requires a unique set-up. As a result, each system must not only be highly flexible, but also able to excel a great multitude of protocols and processes. The Olympus BX3 series of microscopes are just such instruments, offering excellent hardware and software modularity embedded in a flexible imaging system environment and ensuring that, whatever the task, the researcher will always remain in control.
In addition to the ergonomic binocular and trinocular observation tubes, Olympus offers customizable dual-observation and multi-viewing attachments for laboratory discussions. These systems are also invaluable for clinical observations, teaching and instruction, which are much more efficient if the entire group can see the discussion specimen through an individual eyepiece. There is a wide choice, designed for two to ten or even more participants.
The versatile BX53 system is more than a microscope and can easily be tailored to a sophisticated imaging system for any application. From advanced cutting edge research work to optimal stand-alone models for conferencing. Our full line of digital cameras and cellSens imaging software ensure e.g. the capture of fluorescence images with highest S/N ratio.
|Observation Method > Fluorescence (Blue/Green Excitations)||✓|
|Observation Method > Fluorescence (Ultraviolet Excitations)||✓|
|Observation Method > Differential Interference Contrast||✓|
|Observation Method > Phase Contrast||✓|
|Observation Method > Polarized Light||✓|
|Observation Method > Simple Polarized Light||✓|
|Observation Method > Brightfield||✓|
|Observation Method > Darkfield||✓|
|Illuminator > Transmitted Koehler Illuminator > Halogen Lamp|
|Illuminator > Fluorescence Illuminator > Hg Lamp|
|Illuminator > Fluorescence Illuminator > Xenon Lamp|
|Illuminator > Fluorescence Illuminator > Light Guide Illumination|
|Focus > Focusing Mechanism > Stage Focus||✓|
|Intermediate Magnification Changer > Manual Terret||✓|
|Revolving Nosepiece > Motorized||Max. 7 position|
|Revolving Nosepiece > Manual > Coded||Max. 7 position|
|Revolving Nosepiece > Manual > Standard Type||Max. 7 position|
|Stage > Mechanical > Mechanical Stages with Right-Hand Control|
|Stage > Mechanical > Oil Rectangular Stage with Right-Hand Control|
|Stage > Mechanical > Plain Stage||U-SP|
|Stage > Mechanical > Rotatable Graduated Stage|
|Condenser > Motorized > Universal Condenser||Dry: NA0.9/ W.D.1.5 mm, Oil: NA1.4/ W.D.0.63 mm (1.25 X - 100 X)|
|Condenser > Manual > Universal Condenser||Dry: NA0.9/ W.D.1.5 mm, Oil: NA1.4/ W.D.0.63 mm (1.25 X - 100 X)|
|Condenser > Manual > Swing-Out Condenser||NA0.9/ W.D.2 mm (1.25 X - 100 X)|
|Condenser > Manual > Achromatic/Aplanatic Condenser||NA1.4/ W.D.- (10 X - 100 X)|
|Condenser > Manual > Ultra Low Condenser||NA0.16/ W.D.- (1.25 X - 4 X)|
|Condenser > Manual > Darkfield Condenser Dry||NA0.8 - 0.92/ W.D.4.52 mm (10 X - 40 X)|
|Condenser > Manual > Darkfield Condenser Oil||NA1.2 - 1.4/ W.D.0.5 mm (20 X - 100 X)|
|Condenser > Manual > Low Magnification Condenser||NA0.75/ W.D.1.55 mm (2 X - 100 X)|
|Condenser > Manual > Abbe Conenser||NA1.1/ W.D.- (4 X - 100 X)|
|Condenser > Manual > Phase Contrast Condenser||NA1.1/ W.D.0.7 mm (4 X - 100 X)|
|Condenser > Manual > Polarizing Condenser||NA0.9/ W.D.- (4 X - 100 X)|
|Observation Tubes > Widefield (FN 22) > Binocular||✓|
|Observation Tubes > Widefield (FN 22) > Tilting Binocular||✓|
|Observation Tubes > Widefield (FN 22) > Trinocular||✓|
|Observation Tubes > Widefield (FN 22) > Tilting Trinocular||✓|
|Observation Tubes > Widefield (FN 22) > Ergonomic Tilting Binocular||✓|
|Observation Tubes > Widefield (FN 22) > Tilting, Telescopic, Lifting Binocular||✓|
|Observation Tubes > Widefield (FN 22) > Trinocular for Infrared||✓|
|Observation Tubes > Widefield (FN 22) > Erected Trinocular||✓|
|Observation Tubes > Widefield (FN 22) > Erected Ergonomic Tilting Binocular||✓|
|Observation Tubes > Super Widefield (FN 26.5) > Trinocular||✓|
|Observation Tubes > Super Widefield (FN 26.5) > Erect image tilting trinocular||✓|
|Dimensions||274.5 (W) x 614 (D) x 469 (H) mm (Epifluorescence Configuration)|
|Weight||21 kg (Epifluorescence Configuration)|
|Operating Environment > Indoor Use > Ambient Temperature||5 - 40 ºC (41 - 104 ºF)|
|Operating Environment > Indoor Use > Maximum Relative Humidity||80 % for temperatures up to 31 ºC (88 ºF), decreasing linearly through 70 % at 34 ºC (93 ºF), 60 % at 37 ºC (99 ºF), to 50 % relative humidity at 40 ºC (104 ºF)|
|Operating Environment > Indoor Use > Supply Voltage Fluctuations||±10 %|