BX63 Upright Microscope
The BX63 is completely motorized and is uniquely focused via the nosepiece rather than stage, enabling the stage to be fixed making it more stable. The motorized stage is also a new feature and is driven by advanced, high-precision, ultrasonic Piezo technology, providing silent, smooth and extremely precise operation. The stage can even be positioned by hand enabling rapid gross sample alignment while highly accurate encoders continuously read-out the X and Y position. The excellent encoding enables the user to set precise coordinates and navigate directly to them at high speed.
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The BX63 is a motorized microscope developed in response to the requests of hundreds of researchers and microscope users. With a simple touch, users can operate the microscope quickly through the detachable and freely positional U-MCZ controller or by means of a computer with cellSens imaging software*.
All data captured via controlling the microscope through the touch panel or U-MCZ controller are continually fed back through cellSens, providing the freedom to operate the microscope in the most convenient manner for the user. From image capture to report creation, Olympus cellSens personalizes researchers’ individual operational environments in accordance with individual researcher workflows.
* cellSens software is not for clinical diagnostic use
The BX63 is focused by motorizing the nosepiece in the Z-axis (possible due to the infinity-corrected optics) and fixing the stage to the rest of the frame at two additional points, making it extremely stable. In addition to this stability, the motorized nosepiece Z-drive is very accurate, enabling highly precise Z-stack capture and subsequent deconvolution.
Motorizing the nosepiece has the advantage that the objective can be changed using either the remote control handset or the PC software control. This makes imaging far more efficient, freeing the user up to perform other tasks. Also, the more advanced imaging techniques may require the use of multiple magnifications and, with a motorized nosepiece and cellSens software, the whole process can be automated. A further benefit is that the correct objective is selected every time and the magnification is recorded with each image.
Higher signal-to-noise ratio (S/N) produces fluorescence images with bright colors and dark background, that’s why Olympus has pushed the boundaries of S/N with improved fluorescence detection capacity. Uniform illumination and detection is achieved through the use of high transmission objectives, mirror units, and the incorporation of a fly-eye lens system.
It is important to ensure that the field of view is illuminated evenly. The nature of fluorescence illumination makes this a difficult task, but Olympus has developed a new solution with its fluorescence illumination concept, which incorporates a fly-eye lens system. As a result of this advance, not only are users assured of flawlessly homogeneous illumination across the entire wavelength spectrum, but they also have the benefit of much simpler burner alignment.
The Olympus 8-position fluorescence illuminator allows greater flexibility for a wide variety of fluorescence specimens with its easily exchangeable mirror units. Observations are accelerated further through the reduced need to replace mirror units for multicolor or FISH applications.
The UIS2 fluorescent mirror cube range is optimized for fluorescence imaging. High quality coatings used on the mirror cubes provide excellent transmission and steep cut-off slopes, while the interior surfaces eliminate over 99 % of stray light, ensuring the highest sensitivity and best color separation. The mirror cubes can be exchanged quickly and easily without tools.
At the heart of any light microscope is the quality of the optical system. Olympus UIS2 optical components have been developed to provide the perfect optical system, setting new standards in precision and clarity. The high NA objectives lenses are optimally corrected for chromatic aberrations and deliver high resolution, with even the faintest of signals being collected. By using carefully selected raw materials for glass, and applying advanced UW multi-coatings technology, Olympus has
reduced objective autofluorescence and significantly improved the S/N ratio.
UW multi-coatings also yields a flat, high transmission over a wide wavelength range, ensuring high performance in research tasks using different types of fluorochromes.
The motorized universal condenser is designed to reduce back-reflections and autofluorescence by swinging its top lens out, automatically closing its diaphragm to the minimum, and locating the wheel in between two positions in fluorescence imaging.
The ability to reduce auto-fluorescence normally associated with immersion oil makes this product well suited for fluorescence microscopy. Reduced noise (auto-fluorescence) results in an increased signal-to-noise ratio for better fluorescent observation. Temporal change of auto-fluorescence is less. Especially useful in quantitative observation of single molecule fluorescence, which is easily affected by noise. Resistance to crystallization allows it to be used over long periods of time. The refraction index is the same as other Olympus products, assuring easy integration with your existing microscopy system.
At the microscope level, biological samples tend not to possess inherent contrast, such as color variations, when using standard brightfield illumination. As a result, a number of different ways of generating contrast have been developed. These can be split into two parts: optical contrast methods and sample contrast methods. Whatever the source of contrast, the Olympus BX3 range and UIS2 optical components perform flawlessly, providing sharp and clear images in any contrast method.
Straining to view live images while adjusting the microscope is not necessary. With the BX63 and Olympus cellSens software*, flexibility is provided to place the touch panel and U-MCZ remote microscope controller anywhere on the bench, enabling quick and efficient image focusing and framing with the U-MCZ positioned close to the monitor. Simple and quick switching between observation methods and magnifications is provided by the cellSens Process Manager. This automates multicolor,
multipoint and other imaging methods from settings entered through the touch panel or directly into cellSens. After acquisition, image processing, measurement and analysis is completed through the Olympus cellSens software.
* cellSens software is not for clinical diagnostic use
The Olympus BX63 has a motorized ND filter wheel for fluorescence and transmitted light intensity adjustments. Special adapters are required for mounting (U-LHEAD for fluorescence, and U-LH100ADP for transmitted light).
This revolving nosepiece (U-D7REA) allows simultaneous attachment of seven objectives. It is especially suitable for continuous observations from low to high magnifications and combining specific objectives, such as polarized light observations.
By integrating with designated optical components, the motorized universal condenser (BX3-UCD8A) accommodates various kinds of transmitted light observation, from brightfield to differential interference contrast and phase contrast.
The ultrasonic stage (BX3-SSU) delivers high-precision XY control. The XY controller can be mounted on the controller/U-MCZ for the BX63 and worked like conventional stage handles.
The BX63 can switch between different observation methods and magnifications with one touch. The controller offers the choice of a Guidance Mode enabling navigation of procedures currently in use, and the Full Operations Mode that provides access to an entire range of settings - keep full control close at hand for prompt view of microscope system status. Furthermore, multiple observation points and conditions can be saved in advance, enabling rapid recall and reproduction of imaging conditions used by other researchers.
The U-MCZ controller can be detached from the microscope frame and positioned wherever required. When used in combination with the XY controller, provided with the ultrasonic stage, it delivers user-friendly focusing and framing operations that mimic a conventional microscope while live images in the Olympus cellSens software are kept clearly in view. Controls are also conveniently located to enable switching between different observation methods, objectives, and mirror units while retaining the ability to simultaneously select intensity adjustments or image capture.
With microscope optics pushing the boundaries of resolution at all magnifications and new microscope design enabling techniques, it is important to be able to efficiently capture and process the images produced. In addition, an increasing number of researchers are using microscopy and it is, therefore, essential that imaging and analysis are both flexible and user-centric.
Capture multi-color, time lapse, and z-stack images with ease. Just select the appropriate capture button, add relevant parameters, and click "Start". The Process Manager or Graphical Experiment Manager(GEM) make it easy to capture multidimensional image. The GEM function allows experiments to be designed with even greater versatility. Furthermore, image acquisition is available for up to 6 dimensions (XYZTλ multipoint).
* cellSens software is not for clinical diagnostic use
The versatile BX63 system is more than a microscope and can easily be tailored to a sophisticated imaging system for any application. From advanced cutting edge research to optimal stand-alone models for conferencing. Our full line of digital cameras and cellSens imaging software ensure e.g. the capture of fluorescence images with highest S/N ratio.
|Observation Method > Fluorescence (Blue/Green Excitations)||✓|
|Observation Method > Fluorescence (Ultraviolet Excitations)||✓|
|Observation Method > Differential Interference Contrast||✓|
|Observation Method > Phase Contrast||✓|
|Observation Method > Simple Polarized Light||✓|
|Observation Method > Brightfield||✓|
|Observation Method > Darkfield||✓|
|Illuminator > Transmitted Koehler Illuminator > LED Lamp|
|Illuminator > Transmitted Koehler Illuminator > Halogen Lamp|
|Illuminator > Fluorescence Illuminator > Hg Lamp|
|Illuminator > Fluorescence Illuminator > Xenon Lamp|
|Illuminator > Fluorescence Illuminator > Light Guide Illumination|
|Focus > Focusing Mechanism > Nosepiece Focus||✓|
|Focus > Motorized|
|Intermediate Magnification Changer > Manual Terret||✓|
|Revolving Nosepiece > Motorized||Max. 7 position|
|Stage > Motorized > BX3-SSU Ultrasonic Stage for BX3|
|Stage > Mechanical > Mechanical Stages with Right-Hand Control|
|Stage > Mechanical > Oil Rectangular Stage with Right-Hand Control|
|Condenser > Motorized > Universal Condenser||Dry: NA0.9/ W.D.1.5 mm, Oil: NA1.4/ W.D.0.63 mm (1.25 X - 100 X)|
|Condenser > Manual > Universal Condenser||Dry: NA0.9/ W.D.1.5 mm, Oil: NA1.4/ W.D.0.63 mm (1.25 X - 100 X)|
|Condenser > Manual > Swing-Out Condenser||NA0.9/ W.D.2 mm (1.25 X - 100 X)|
|Condenser > Manual > Achromatic/Aplanatic Condenser||NA1.4/ W.D.- (10 X - 100 X)|
|Condenser > Manual > Ultra Low Condenser||NA0.16/ W.D.- (1.25 X - 4 X)|
|Condenser > Manual > Darkfield Condenser Dry||NA0.8 - 0.92/ W.D.4.52 mm (10 X - 40 X)|
|Condenser > Manual > Darkfield Condenser Oil||NA1.2 - 1.4/ W.D.0.5 mm (20 X - 100 X)|
|Observation Tubes > Widefield (FN 22) > Binocular||✓|
|Observation Tubes > Widefield (FN 22) > Tilting Binocular||✓|
|Observation Tubes > Widefield (FN 22) > Trinocular||✓|
|Observation Tubes > Widefield (FN 22) > Tilting Trinocular||✓|
|Observation Tubes > Widefield (FN 22) > Ergonomic Tilting Binocular||✓|
|Observation Tubes > Widefield (FN 22) > Tilting, Telescopic, Lifting Binocular||✓|
|Observation Tubes > Widefield (FN 22) > Trinocular for Infrared||✓|
|Observation Tubes > Widefield (FN 22) > Erected Trinocular||✓|
|Observation Tubes > Widefield (FN 22) > Erected Ergonomic Tilting Binocular||✓|
|Dimensions||294.5 (W) x 740.5 (D) x 582.5 (H) mm (Epifluorescence Configuration)|
|Weight||35 kg (Epifluorescence Configuration)|
|Operating Environment > Indoor Use > Ambient Temperature||5 - 40 ºC (41 - 104 ºF)|
|Operating Environment > Indoor Use > Maximum Relative Humidity||80 % for temperatures up to 31 ºC (88 ºF), decreasing linearly through 70 % at 34 ºC (93 ºF), 60 % at 37 ºC (99 ºF), to 50 % relative humidity at 40 ºC (104 ºF)|
|Operating Environment > Indoor Use > Supply Voltage Fluctuations||±10 %|