MetaMorph for Olympus is an advanced digital imaging software for capture, display, and analysis of biological images. Built on the market-proven MetaMorph platform, this powerful package offers functionality, flexibility, and stability that meet the most demanding image acquisition and analysis needs. With over 10,000 publications citing MetaMorph, you can rest assured that your imaging needs will be met now and in the future.
The MetaMorph for Olympus is not for clinical diagnostic use.
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Proven Performance for Image Capture Display and Analysis
MetaMorph for Olympus converts your biological experiment into a publishable result. Built on the market-proven MetaMorph platform, this powerful package offers functionality, flexibility, and stability that meet the most demanding image acquisition and analysis needs. With over 10,000 publications citing MetaMorph, you can rest assured that your imaging needs will be met now and in the future.
Acquisition & Device Control
MetaMorph for Olympus device control makes acquisition fast and easy, allowing the integration of IX2 and BX3 microscopes and a variety of other hardware devices into a single, intuitive acquisition interface.
Image Display & Processing
Image display and processing tools are crucial for performing accurate image analysis, and MetaMorph for Olympus offers a complete toolbox of advanced features in this area, from background subtraction and shading correction, to morphology filters and an interactive 4D Viewer.
MetaMorph for Olympus offers analysis tools to handle everything from simple intensity logging to advanced morphometry analysis, colocalization, FRET, 3D measurements, and more. Optional Application Modules perform assay-specific analysis, such as counting nuclei or assessing cell cycle phases, in a simple wizard-like interface.
Powerful and easy-to-use journal (macro) functions in MetaMorph for Olympus enable users to further automate acquisition, processing, and common analysis routines, while custom toolbars and menus provide users quick access to commonly used menu options, taskbars, and journals.
The MetaMorph for Olympus is available for both acquisition and offline (analysis only) needs.
MetaMorph for Olympus Acquisition Features
Drivers for many popular CCD cameras, Olympus IX2 and BX3 motorized microscopes plus shutters, filterwheels, and XY stages from various manufacturers
Multi-dimensional acquisition interface
MetaMorph for Olympus Acquisition Options
Simultaneous and split camera acquisition
Automated slide scanning and stitching
Multi-well plate interface
Control of various devices for FRAP, uncaging, photobleaching and photoablation devices
MetaMorph for Olympus Acquisition and Offline Features
Multi-dimensional data display interface
Image processing features such as shading correction and kernel filters
Integrated Morphometry Analysis for detection and analysis of objects
Colocalization and FRET tools
MetaMorph for Olympus Acquisition and Offline Options
Multi-well data viewing
Application Modules for specialized application analysis needs
Additionally, we offer MetaFluor for Olympus for ratio imaging of intracellular ion concentrations.
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MetaMorph for Olympus offers eleven user-friendly Application Modules for biology-specific analysis. Each Application Module features a dedicated dialog box with intuitive setting selections, improved segmentation through adaptation to local content, and both field and cell-by-cell data logging. After analysis is complete, users can interactively view tabular and image results side by side. Application Modules may be incorporated into macros for increased customization and automation of analysis. All Application Modules are validated in-house and with third-party collaborators.
Angiogenesis Tube Formation
Multi Wavelength Cell Scoring
Kinase activation (Cell Scoring)
Fatty acid uptake (Cell Scoring, Multi Wavelength Cell Scoring)
Mitosis (Mitotic Index, Cell Cycle)
Adipogenesis (Cell Scoring, Multi Wavelength Cell Scoring)
Examining transfection efficiencies (Cell Scoring, Multi Wavelength Cell Scoring)
Studying intracellular structures (Granularity)
Receptor internalization (Granularity)
Clustering target molecules(Granularity)
Process extension (Neurite Outgrowth)
Neurodegenerative or neuroregenerative diseases (Neurite Outgrowth)
Cell differentiation (Stem cell research) (Neurite Outgrowth)
Protein expression & modification (Cell Scoring, Multi Wavelength Cell Scoring)
Transient transfection (Cell Scoring, Multi Wavelength Cell Scoring)
Detect monopolar spindles (Monopole Detection)
Cell signaling (Cell Health, Cell Cycle)
Fluorescence ratio imaging is the monitoring of live cells in which a fluorescent indicator of intracellular ions is introduced. Indicator dyes have been designed to shift their fluorescence excitation or emission spectrum when binding with specific ions. Images are obtained at two different wavelengths, typically matching the absorption bands at the high and low binding conditions.
By rationing the intensities in the images, it is possible to construct a map showing the local ion concentrations throughout the field of view. Since the monitoring process is nondestructive, image acquisition can be repeated frequently to trace and monitor the time course of cellular responses.
MetaFluor for Olympus is designed for dual-wavelength intracellular ion measurements. The system provides simultaneous display of the raw data, ratio image, graphs of intensities, ratios and ion concentrations, and a non-ratiometric image such as a brightfield or phase-contrast image. Two different ratiometric indicators can be imaged and measured simultaneously.
Toolbars, menus, wizards and dialog boxes help move you through the image processing steps quickly. Features such as multiple image windows, flexible device control, synchronization and timing, and journals allow for automated image acquisition and analysis unlike any other system.
With MetaFluor for Olympus, you customize the set-up once, then let the experiment run by itself. You are able to collect a large amount of data online and process it with either MetaFluor for Olympus or an analysis-only copy of the software.
Monitoring of Live Cells
Regions of interest can be generated automatically or manually placed on your image to monitor intensity, ratio value or ion concentration. Measurements are then made simultaneously on all the regions of interest and update continuously on a scrolling graph, allowing you to follow dynamic changes as they occur in your living samples.
A display of multiple graphs gives flexibility in the presentation of your experiment's data. MetaFluor enables you to click on graph traces to display a readout of the time and data value for the region nearest to the click.
The Event Mark function is useful to record when drugs or solutions are added, experimental conditions changed, triggers are received or sent or other events occur. You have the option to associate a timer and an alarm bell to each event. Additionally, for perfused samples, ambient conditions can be logged and tracked. Each image has an annotation that is saved within the TIFF file format. The annotation will record wavelength-dependent settings. Additional information can be stored in a protocol file.
Export Data for Analysis
If needed, MetaFluor for Olympus can log and export all measurements to either a text file or to a spreadsheet program such as Microsoft Excel.
Compatible with MetaMorph for Olympus
Because MetaFluor for Olympus saves images in TIFF file format, you can import them into MetaMorph for Olympus for further processing and analysis.
Presentation and Publication
Images in MetaFluor for Olympus can be displayed in monochrome, pseudocolor, or using a variety of user-defined set of values. Ratio images can also be displayed using a special display mode called Intensity Modulated Display, or IMD. With the IMD mode, color is used to represent the relative ratio value, while the intensity or brightness of the color is used to represent whether the brightness of the source images. This technique helps automate the process of extracting spatial information from the background, by automatically eliminating background fluorescence from the scene. MetaMorph and MetaFluor are trademarks of Molecular Devices, Inc.
Out-of-focus intensities are present in all acquired images. These intensities can be accounted for by observing the behavior of light originating in a point source and passing through the microscope optics. This behavior is described by the Point Spread Function (PSF). It can be used to quantitatively compensate for the blurring of images due to out-of-focus information. This process is called deconvolution. MetaMorph's 3D Deconvolution module from AutoQuant helps improve images by reassigning out-of-focus intensities back to the spatial locations to where they originated. The results are images with sharper definition and lower background, better contrast, and improved signal-to-noise ratio.
The 4D Viewer enables the visualization of multi dimensional data sets, time lapse or Z stacks. Users can simultaneously view multiple Z sections as a 3D reconstruction, with multiple , wavelengths, time points, and positions in a single intuitive viewing window. Users can interactively rotate the 3D view and obtain volumetric measurements. A 3D model consisting of rotated views from a stack of images can also be created. Using a stack of planes from a Z-series, users may configure the angle, orientation, Z-axis distance, and reconstruction type for the model.
Cell Migration/Cell Proliferation
The Count Nuclei Application Module is designed to automate accurate counting of nuclei for most types of cells. The module counts nuclei even when the background is uneven, providing superior segmentation compared to simple thresholding. This module can be used to count nuclei across large data sets and log the results to a spreadsheet.
For 2D deconvolution, MetaMorph offers No Neighbors and Nearest Neighbors algorithms to remove out-of-focus information from individual planes or a Z-stack. Both modules use an estimated three-dimensional Point Spread Function (PSF). The 3D deconvolution module can be used for analysis of Z-stacks and may be configured to use either a Blind algorithm which estimates a Point Spread Function (PSF) or an algorithm where a measured PSF which characterizes the objective lens is provided.
MetaMorph can perform quantitative densitometry. The software will display the optical densities of a brightfield source image in a scaled 8-bit or 16-bit image.
To better visualize events, up to six fluorescence images can be overlaid over a background Differential Interference Contrast (DIC) or phase contrast image.
Fluorescent in situ Hybridization (FISH)
FISH is used for the detection of target DNA or RNA molecules with a system of coupled fluorochromes. The detection of nucleotidic sequences on a combed DNA molecule is performed indirectly, by first hybridizing the sought nucleotidic sequences with the combed DNA (also called the matrix DNA or target). If the probes are synthesized with incorporated fluorescent molecules or antigenic sites which can be recognized with fluorescent antibodies, the direct visualization of the relative position of the probes is possible. MetaMorph easily automates the process of acquiring, color combining, and visualizing multiple wavelengths from FISH and immunocytochemistry experiments.
Immunocytochemistry is the in situ detection and demonstration of cellular constituents using specific antigen-antibody reactions. It has evolved to become an integral aid in modern histopathology. Crucial to its success has been the development of technologies that have allowed the highly sensitive and reliable detection of cellular markers within routinely fixed and processed samples. MetaMorph for Olympus can acquire, combine, and measure images of one or more wavelengths from immunocytochemistry and in situ hybridization experiments. The morphological measurement tools in MetaMorph are ideal for processing immunohistological tissue samples. MetaMorph for Olympus can acquire, combine, and measure images of one or more wavelengths from immunocytochemistry and in situ hybridization experiments.