1 00:00:03,14 --> 00:00:10,08 In this video, we’ll show you how to set up a project in your CM20 incubation monitoring system. 2 00:00:10,08 --> 00:00:14,21 First, place the culture vessel on the upper left part of the head unit. 3 00:00:14,21 --> 00:00:23,02 If you’re using a microplate, place it so that well number 1 or A1 is in the upper right. 4 00:00:23,02 --> 00:00:28,02 Close the incubator’s doors and log into the CM20 software. 5 00:00:28,02 --> 00:00:30,17 To create a project and start monitoring, 6 00:00:30,17 --> 00:00:35,13 you’ll need to enter some information about the vessel used and acquisition parameters. 7 00:00:35,13 --> 00:00:39,16 First, select the monitoring head to start the project. 8 00:00:39,16 --> 00:00:43,02 Then, click Create Project Setting. 9 00:00:43,02 --> 00:00:46,16 Input the name of the project, 10 00:00:46,16 --> 00:00:50,17 select the cell type, and define the access permissions. 11 00:00:50,17 --> 00:00:55,09 When you’re done, click apply. 12 00:00:55,09 --> 00:01:04,10 Next, select the vessel type, manufacturer, and model. 13 00:01:04,10 --> 00:01:07,13 Click apply to go to the Area Setting. 14 00:01:07,13 --> 00:01:15,01 Here, enter the information about cells, media, and any initial passage number. 15 00:01:15,01 --> 00:01:21,21 Click apply, then select an analysis parameter from the list or import saved parameters. 16 00:01:21,21 --> 00:01:25,22 For first time experiments, select not specified. 17 00:01:25,22 --> 00:01:29,20 Click apply to go to the Monitoring Setting page. 18 00:01:29,20 --> 00:01:33,01 Here, you can select the time to browse the whole area, 19 00:01:33,01 --> 00:01:37,13 start time delay, and the interval time for analysis. 20 00:01:37,13 --> 00:01:41,10 When you’re done, click to start monitoring. 21 00:01:41,10 --> 00:01:46,23 Periodically, you’ll need to exchange the media your cells are growing in. 22 00:01:46,23 --> 00:01:52,02 To do this, quickly press the media exchange button to pause the monitoring. 23 00:01:52,02 --> 00:01:57,00 Remove the culture vessel and replace the media. 24 00:01:57,00 --> 00:02:03,06 Once you’ve put it back, another quick press on the media exchange button will resume monitoring. 25 00:02:03,06 --> 00:02:09,07 When it’s time for cell passage, quickly press the power/passaging button to pause the monitoring. 26 00:02:09,07 --> 00:02:13,01 Remove the culture vessel and pass the cells. 27 00:02:13,01 --> 00:02:15,20 When you’re done, place the vessel back in the incubator 28 00:02:15,20 --> 00:02:20,03 and quickly press the power/passaging button to resume monitoring. 29 00:02:20,03 --> 00:02:23,03 Setting up your project is that simple! 30 00:02:23,03 --> 00:02:27,16 For more information about the CM20 monitoring system or our other products, 31 00:02:27,16 --> 00:02:31,21 visit olympus-lifescience.com.