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In this video, we’ll show you how to analyze the data
from your CM20 incubation monitoring system.


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To start, click on the Analysis Setting tab on the left.

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Click new to select the analysis parameters.

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If you’re using a microplate, select the growth area
you want to analyze first before clicking new.

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From the Select Passage drop-down menu, click on latest.

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The dialog box for setting analysis data is displayed.

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First, let’s create analysis parameters for adherent cells.

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We’ll create settings for calculating cell confluency and cell count.

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To display the acquired images, select the monitoring position.

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To analyze cell confluency, start drawing lines
on the cells and on the background.

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If needed you can zoom in on the image using the wheel on your mouse

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in order to better be able to identify the cells and background area.

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After a few cells and background areas have been identified,
click start learning.

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The system will use its machine learning algorithm
to differentiate between cells and background.

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If necessasry you may add lines and repeat this step.

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Click apply once the algorithm has correctly identified the cells.

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To analyze cell count, set the threshold by moving the slider

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until there’s a single light-blue point per cell. Then click apply.

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If your cells aren’t being identified correctly,
you can fine tune the counting threshold.

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Scroll down to save the created analysis parameter
by inputting a name and clicking save.

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Click apply once you’ve selected the parameter from the list
and then click start analysis.

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Now, let’s create analysis parameters for iPS or ES cells.

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To measure colony confluency,

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specify the cells and background just like you did earlier
for adherent cells.

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Cell count and colony size can be calculated by
adjusting the slider that recognizes each colony.

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When you’re done, click apply.

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Save the created analysis parameters as a setting file.

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You can then start the analysis by selecting
the desired analysis parameter.

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You can display your report from the export tab on the left.

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Here, you can display a project report,


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download data in a CSV format,


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download image data for the whole image or an analysis position,

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or display project settings.

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Click download the whole area.

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Progress for the data download can be seen
by clicking on the download icon in the top right.

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Check the box next to a prepared file and click download to save the data.

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With the CM20 monitoring system, keeping track of your data is easy.

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For more information about this or our other products,

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visit olympus-lifescience.com.