1 00:00:00,000 --> 00:00:05,757 Olympus’ patented TruSpectralTM technology improves spectral detection efficiency. 2 00:00:05,757 --> 00:00:11,345 See how TruSpectral works in the Fluoview 3000 Lightpath to improve your research. 3 00:00:11,345 --> 00:00:15,767 Inside the scanner, the excitation laser beam is guided to the sample 4 00:00:15,767 --> 00:00:19,853 by the excitation dichroic and silver-coated scanning mirrors. 5 00:00:19,853 --> 00:00:24,650 Emission light passes back through the scanner along the same path as the excitation light, 6 00:00:24,650 --> 00:00:29,655 passing through the excitation dichroic and up to the TruSpectral detector deck. 7 00:00:29,655 --> 00:00:34,285 At the detector, TruSpectral technology employs a volume phase hologram 8 00:00:34,285 --> 00:00:39,748 to diffract light with up to three-fold higher transmission efficiency versus reflection gratings. 9 00:00:39,748 --> 00:00:43,587 A motorized adjustable slit in front of the TruSpectral detector 10 00:00:43,587 --> 00:00:47,465 creates bandwidths from 1 nanometer up to 100 nanometers, 11 00:00:47,465 --> 00:00:51,385 giving users maximum control over the light they collect. 12 00:00:51,385 --> 00:00:55,182 The FV3000 microscope’s TruSpectral technology 13 00:00:55,182 --> 00:00:58,350 brings new levels of total system transmission efficiency, 14 00:00:58,350 --> 00:01:02,147 enabling every system to be completely spectral. 15 00:01:02,147 --> 00:01:05,942 With up to four dynamic ranges, bright and dim spectral signals 16 00:01:05,942 --> 00:01:11,823 can be easily separated by independently adjusting the sensitivity of each detector.