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In this video, we'll show you how to use FLUOVIEW™ FV3000 software

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to create a Z stack projection, crop an image,
and export your image as other file formats.

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FV3000 software simplifies the processing
of large multidimensional data sets.

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Your images can be processed by selecting
the analysis tool window.

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The analysis work space will appear to your right.

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To create a Z stack projection, double click Projection
from the processing Items list.

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Select the appropriate file to process.

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Use the Processing Property options
to select the desired Z planes and projection type.

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In this example, we've chosen a maximum intensity projection
by Z for all planes and channels.

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Clicking Process in the lower right corner initiates
the analysis process, and a new file will appear.

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Here, we see the resulting projection
of a stitched rat brain tissue section.

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To crop or extract a particular subregion from a large data set,

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use the Analysis ROI tools to select the area of interest.

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To create a new file of just this subregion,

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remove your previous task and double click
Extraction from the processing items list.

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Select True for Use Clip ROI in the processing properties window.

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Make sure your selections for channels and dimension
are applicable to your needs.

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Selecting Process will open a new image window

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that has been autosaved to the hard drive as
an Olympus .oir file format.

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It's also possible to export your image as alternative file formats.

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To do so, select export from the file menu.

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A new window will appear allowing you to define a save location.

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You can select a file through the Windows browser.

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Enter your desired file name.

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Select the channels you'd like to export.

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Choose your desired output format.

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This check box will include a file containing
the image's metadata.

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Thanks for watching.

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To learn more about the FLUOVIEW FV3000 confocal microscope

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or other Olympus Life Science solutions,
visit olympus-lifescience.com.