1 00:00:01,920 --> 00:00:05,382 This video will show you how to apply oil to your objective 2 00:00:05,382 --> 00:00:10,553 on the IX83 microscope with an IX3-SSU stage. 3 00:00:10,553 --> 00:00:15,058 Olympus offers two types of immersion oil, depending on the type of objective. 4 00:00:15,058 --> 00:00:21,147 The blue bottle contains regular immersion oil, and the green bottle contains silicone immersion oil. 5 00:00:21,147 --> 00:00:25,485 Make sure that you are using the appropriate type of oil for your objective. 6 00:00:25,485 --> 00:00:28,237 To start, using a low-power objective, 7 00:00:28,237 --> 00:00:32,283 bring the specimen into focus and find your region of interest. 8 00:00:32,283 --> 00:00:35,370 Then, select the Stage tab on the TPC, 9 00:00:35,370 --> 00:00:39,332 and select “SET” in the lower-righthand corner of the tab. 10 00:00:39,332 --> 00:00:42,043 This will save your current position. 11 00:00:42,043 --> 00:00:45,547 Then select the oil immersion objective you wish to use, 12 00:00:45,547 --> 00:00:48,883 and press "ESCAPE" to retract the objective. 13 00:00:48,883 --> 00:00:53,763 Gently move the SSU into a position where you can access the objective. 14 00:00:53,763 --> 00:00:59,060 Place a small drop of oil on the objective, taking care to avoid creating bubbles. 15 00:00:59,060 --> 00:01:02,230 For regular oil, a small drop will suffice. 16 00:01:02,230 --> 00:01:06,483 For silicone oil, a more liberal drop should be applied. 17 00:01:06,483 --> 00:01:10,988 After applying the oil, select “GO” in the bottom-right corner of the Stage tab 18 00:01:10,988 --> 00:01:15,033 on the TPC to bring the stage back to its original position. 19 00:01:15,033 --> 00:01:19,538 Then press “ESCAPE” to release the objective from its retracted position. 20 00:01:19,538 --> 00:01:25,712 Use the eyepieces and the U-MCZ remote controller to perform any final focus adjustments.