1 00:00:04,19 --> 00:00:08:12 In this series, we’ll show you how to use the Well Plate Navigator, 2 00:00:08,12 --> 00:00:14:00 a simple, effective tool for well plate acquisition available in cellSens software. 3 00:00:14,00 --> 00:00:18:03 The Well Plate Navigator supports a variety of well plates. 4 00:00:18,03 --> 00:00:21:13 It can easily create or copy acquisition patterns... 5 00:00:21,13 --> 00:00:27:18 and group wells for complex experiment settings and easy data handling. 6 00:00:27,18 --> 00:00:32:12 In this video, you’ll learn how to prepare to use the Well Plate Navigator. 7 00:00:32,12 --> 00:00:36:10 If you already know how, skip to part 2 of this series. 8 00:00:36,10 --> 00:00:38:08 Let’s get started. 9 00:00:38,08 --> 00:00:41:15 First, set the stage limit calibration. 10 00:00:41,15 --> 00:00:49:18 Click ‘Calibrations’ on the Start Page or ‘Acquire’ on the Menu bar. 11 00:00:49,18 --> 00:00:52:13 Double click ‘Stage Limits.’ 12 00:00:52,13 --> 00:00:55:08 Then, click ‘Next.’ 13 00:00:55,08 --> 00:01:00:02 Check all the boxes and click ‘Next.’ 14 00:01:00,02 --> 00:01:04:16 Now, we can set the XY limits of the stage in the software. 15 00:01:04,16 --> 00:01:08:03 Let’s start with the maximum Y value. 16 00:01:08,03 --> 00:01:13:11 Continue pressing the arrow button until the stage reaches its mechanical limit. 17 00:01:13,11 --> 00:01:16:12 Then click ‘Y Max.’ 18 00:01:16,12 --> 00:01:21:02 Repeat this process for X Max, Y Min, and X Min. 19 00:01:21,02 --> 00:01:25:23 If you already know the values, you can enter them directly in each box. 20 00:01:25,23 --> 00:01:32:21 Now, the stage can drive to the full range of its mechanical limits. 21 00:01:32,21 --> 00:01:35:14 Please click ‘Next.’ 22 00:01:35,14 --> 00:01:38:05 Let’s calibrate the Z limit. 23 00:01:38,05 --> 00:01:41:00 Here, we can set the software limit of the Z motor 24 00:01:41,00 --> 00:01:44:14 to make sure you can focus on your well plate. 25 00:01:44,14 --> 00:01:47:21 Select the observation method and either focus on your sample 26 00:01:47,21 --> 00:01:53:01 or on the top surface of the well plate using transmitted light observation. 27 00:01:53,01 --> 00:01:55:01 Drive the Z motor up slightly, 28 00:01:55,01 --> 00:02:03:05 and then click ‘Read Current Z Position’ to set the limit. 29 00:02:03,05 --> 00:02:05:15 Click ‘Next,’ 30 00:02:05,15 --> 00:02:09:14 then ‘Finish,’ 31 00:02:09,14 --> 00:02:11:23 and then ‘Close.’ 32 00:02:11,23 --> 00:02:15:13 Now, let’s register and calibrate the well plate. 33 00:02:15,13 --> 00:02:20:03 Click ‘Well Navigation’ in the Layout tab. 34 00:02:20,03 --> 00:02:26:23 Make sure that the lowest-power objective lens is selected. 35 00:02:26,23 --> 00:02:29:19 Select the transmitted light observation method. 36 00:02:29,19 --> 00:02:37:01 Then, click ‘New Well Plate.’ 37 00:02:37,01 --> 00:02:42:05 You can choose from a variety of well plates. 38 00:02:42,05 --> 00:02:45:01 If your plate isn’t listed, select ‘Custom’ 39 00:02:45,01 --> 00:02:52:12 and input the number of rows and columns, the well shape, and numbering. 40 00:02:52,12 --> 00:03:00:15 In this example, we’ll select a standard 96-well plate and click ‘Create.’ 41 00:03:00,15 --> 00:03:06:05 The calibration process will begin automatically. 42 00:03:06,05 --> 00:03:11:02 We’re going to register 3 points at 3 wells for calibration. 43 00:03:11,02 --> 00:03:17:13 Drive the stage to position A1, which is in the upper left of the well plate, using the stage handle. 44 00:03:17,13 --> 00:03:23:13 Drive the stage to the edge of the well and click ‘Confirm position.’ 45 00:03:23,13 --> 00:03:29:09 Then drive the stage to another edge of well A1 and click ‘Confirm position.’ 46 00:03:29,09 --> 00:03:40:21 Do this a third time so that all 3 positions are registered in well A1. 47 00:03:40,21 --> 00:03:47:21 Next, drive the stage to the upper right well, which is A12 in a 96-well plate. 48 00:03:47,21 --> 00:03:54:14 Clicking the upper right part of the Stage Navigator tool window may be easier than using the stage handle. 49 00:03:54,14 --> 00:04:04:09 Confirm you’re observing well A12, then register 3 more positions. 50 00:04:04,09 --> 00:04:21:13 Repeat this process in the lower right well, H12. 51 00:04:21,13 --> 00:04:26:21 Once you’ve registered 3 positions in 3 wells, the calibration process is complete, 52 00:04:26,21 --> 00:04:31:17 and the icons in the Well Navigator tool window are now active. 53 00:04:31,17 --> 00:04:36:06 You’ll see a yellow circle on the Stage Navigator tool window. 54 00:04:36,06 --> 00:04:39:14 This indicates the edge of the current well. 55 00:04:39,14 --> 00:04:42:21 It’s similar to watching a live image. 56 00:04:42,21 --> 00:04:46:11 You can click on a box in the Well Navigator tool window matrix 57 00:04:46,11 --> 00:04:53:12 to drive the stage to the center of the respective well. 58 00:04:53,12 --> 00:04:58:06 You can also use the arrows to move the stage to adjacent wells. 59 00:04:58,06 --> 00:05:13:14 For added convenience, you can save your current calibration as an overview area. 60 00:05:13,14 --> 00:05:18:07 You can register a variety of well plate types and load them anytime, 61 00:05:18,07 --> 00:05:38:08 so you don’t need to repeat the calibration process each time you use the Well Navigator. 62 00:05:38,08 --> 00:05:40:05 Thanks for watching! 63 00:05:40,05 --> 00:05:43:18 Make sure to check out the next video in the Well Navigator series 64 00:05:43,18 --> 00:05:46:09 where I’ll explain how to acquire data. 65 00:05:46,09 --> 00:05:51:12 For more information, visit olympus-lifescience.com.