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Visualizing the Proximity Between the Microtubule Network and the Nucleus in RK13 Cells with Immunofluorescence

Visualizing the Proximity Between the Microtubule Network and the Nucleus in RK13 Cells with Immunofluorescence

The details of the microtubule network were visualized in a culture of RK13 epithelial cells by employing immunofluorescence. The culture was labeled with mouse anti-alpha-tubulin followed by a secondary antibody (goat anti-mouse IgG) conjugated to Alexa Fluor 488. TO-PRO-3 (pseudocolored red) was utilized as a nuclear counterstain.

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