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Fluorochrome Data Tables

The data tables below contain reference information on fluorescence cubes offered by Olympus America, Inc. to support their microscopes. The codes for the excitation columns in Tables 2 are listed in Table 1. Table 2 is a listing of examples of cubes’ configuration of filters and mirrors. The authors wish to caution that we believe this data to be accurate, but it should always be verified by authorized Olympus representatives.

Filter Color Codes
Code Filter Type
U Ultraviolet
V Violet
BV Blue-Violet
B Blue
IB InterferenceBlue Filter
G Green
IG Immuno GoldStaining Method
BF Brightfield,Phase Contrast,and DIC only
Table 1

Table 2 lists the excitation and barrier filters by cube along with the dichroic mirror used in each cube. Use this chart as an aid to guide you in selection of some of the Olympus U-URA cubes for applications listed in our fluorescence and fluorochrome data tables.

Fluorescence Filter Cubes

Discover how variations in excitation and barrier filters allow certain wavelengths to pass.

 Example configuration of Olympus Cubes
Excitation Type Excitation Filter(nm) Dichroic Mirror(nm) Barrier Filter
U Wide 330-385 400 420--
U Narrow 360-370 400 420--
U Narrow Bandpass 360-370 400 420-460
V Narrow 400-410 455 455--
BV Wide 400-440 455 475--
BV Narrow 420-440 455 475--
B Wide 450-480 500 515--
IB Wide, Interference 460-490 505 515IF--
IB Narrow, Interference 470-490 505 515IF--
G Super Wide 480-550 570 590--
G Wide 510-550 570 590--
G Narrow 530-550 570 590--
IG Narrow, Interference 520-550 565 580IF--
IY Wide, Interference 545-580 600 610IF--
Table 2
 

Contributing Authors

Mortimer Abramowitz - Olympus America, Inc., Two Corporate Center Drive., Melville, New York, 11747.

Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.

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