Life Science Solutions

Ask the Experts 03

Ask the Experts

Welcome to Ask the Experts, your one-stop portal for life science microscopy queries. Join our experts on live webinars to discuss trending topics, or contact our experts at any time with your questions and challenges. Missed a webinar? No problem, you can listen again. Don’t see a topic you’d like to discuss? Use our feedback form or contact our experts directly for help.

Live Webinars | On-demand Webinars | Experts

Modern Slide Scanning: Single-cell Phenotyping on Fixed Samples (Encore Edition)

Experts
Flavio Giacobone
Strategic Marketing Manager
Alec De Grand
Product Manager
Angela Vasaturo
Senior Field Application Scientist, Ultivue, Inc.
Get another chance to learn about the latest generation of slide scanners on November 18, when we replay our webinar with our Olympus experts Flavio and Alec. In this webinar, our experts will discuss how modern slide scanners can be a workhorse for your applications and how multiplexing can reveal much more information from a slide. With a guest expert from Ultivue, you’ll learn how single-cell phenotyping can dramatically improve the understanding of the dynamics in a sample, even if it is a fixed slide.

Live Demo: FLUOVIEW™ FV3000 Confocal Laser Scanning Microscope

Experts
James Lopez
National Applications Manager
Olympus America Inc.
Join James Lopez, Ph.D., National Applications Manager to see how the FV3000 confocal laser microscope can expand your research possibilities and help you get more data from your samples.

Whole-Brain Functional Calcium Imaging Using Light Sheet Microscopy

Experts
Ruben Portugues
Institute of Neuroscience
Technical University of Munich
Light sheet microscopy is a powerful technique to perform fast volumetric imaging. I will talk about how we use it to investigate how the brain of a small vertebrate, the larval zebrafish, works. In our laboratory at the Technical University of Munich, we are interested in how the brain processes external sensory stimuli and uses internal states and past experiences to select appropriate behavior. In order to do this, we image the activity of almost all 100,000 neurons in the brain of larval zebrafish while we present the animals with stimuli and monitor their behavior. I will also discuss the data processing steps after acquiring these large datasets.

Live Demo: FLUOVIEW™ FV3000 Confocal Laser Scanning Microscope

Experts
Bülent Peker
Senior Product Marketing Manager
Olympus Europa
Join Bülent Peker, Senior Product Marketing Manager to see how the FV3000 confocal laser microscope can expand your research possibilities and help you get more data from your samples.

In-Vivo Tracking of Harmonic Nanoparticles by Means of a TIGER Widefield Microscope

Experts
Laura Vittadello
Department of Physics and Center of Cellular Nanoanalytics
Osnabrück University
In-vivo tracking based on harmonic nanoparticles is so far not exploited because of a lack of an appropriate tool—a widefield nonlinear optical microscope. Here, we present a new approach to overcome this challenge based on a redesign of laser space parameters.

Live Demo: SLIDEVIEW™ VS200 Research Slide Scanner

Experts
Wei Juan Wong
Application Specialist
Olympus Soft Imaging Solutions
In this live demo you will learn how to capture high-resolution images of your slides for quantitative analysis, enabling you to make the most of the information your slides have to offer. Easily analyze, share, and archive your data with the SLIDEVIEW VS200 digital slide scanner. Join this session and learn how to achieve more in less lead time.

A New Way of Thinking—Object Detection with Deep Learning

Experts
Akira Saito
Assistant Manager, Marketing and Applications
Olympus Singapore
In this session, we will discuss object segmentation with deep learning and its applications in life science. We will also demo Olympus deep-learning software.

Evolution of Scientific Digital Imaging Technologies and their Applications

Experts
Lin Guo
Manager, Product and Application Life Science Department Scientific Solutions Business Division
Olympus Singapore
In this talk, I will cover some of the critical facts about scientific digital cameras. I will also talk about the evolution of these cameras, the solution that Olympus offers, and how they are used in current advanced microscopy systems for various applications.

Confocal Microscopy and Its Use for a Spaceflight Experiment

Experts
Bob McLean
Regents’ Professor
Texas State University
Spaceflight experiments represent a rare but exciting scientific opportunity. Unlike most lab experiments, in which protocols can be quickly modified, limitations on crew time and availability of supplies are notable factors. Unanticipated changes to launch and reentry schedules are also an issue. The experimental apparatus and protocols used must be able to function in a microgravity setting, while also resisting the g-forces and vibrations during launch and landing. During this presentation, Dr. McLean will go over the experimental planning and use of confocal and electron microscopy approaches and analyses during a recent spaceflight experiment that flew on Space X-21 from 12/6/20 – 1/14/21

Deep Learning Approaches to Automated Phenotypic Profiling

Experts
Jesse Chao
Scientist
Sunnybrook Research Institute
Quantifying cellular phenotypes is the key to all cell biology studies. However, modern imaging techniques can easily generate more data than an average user can comfortably handle. In this presentation, I will discuss two deep learning approaches, one semi-supervised and one supervised, for building image analysis pipelines. Either approach can be run on a free cloud GPU instance.

Human Pluripotent Stem Cell-Derived Liver Organoid Manufacturing

Experts
Yosuke Yoneyama
Assistant Professor
Institute of Research, Tokyo Medical and Dental University
Despite its translational value, organoid-based approaches are generally inefficient and irreproducible with the burden of long-term cell culture. In the case of organoids generated from human pluripotent stem cells, including induced pluripotent stem cells (iPSCs), there is a need to monitor the multiple steps that cover iPSC maintenance, two-dimensional differentiation, and three-dimensional morphogenesis. By combining a live cell monitoring system and biochemical assays, we developed a reproducible protocol for generating human liver organoids that minimizes inter-donor-dependent variabilities. Applying this setup to over 15 human iPSC libraries enabled us to investigate the genotype-phenotype relationship in liver organoids, revealing important risk gene polymorphisms for steatohepatitis and fibrosis. These results will help to build a highly reproducible organoid platform to study personalized mechanisms of health and diseases.

Deconvolution of 3D Image Stacks

Experts
Heiko Gäthje
Senior Trainer, Training Academy
Olympus Europa
Images taken with a light microscope are never true representations of the specimen. Error sources, which have to be controlled, are sample preparation and staining protocols as well as optical aberrations and limitations of microscope and digital camera.

Live Demo: IXplore™ SpinSR Confocal Super Resolution System

Experts
Stefan Marawske
Senior Sales Specialist, High-End Life Science Systems
Olympus Europa
In this live demo, experience the IXplore SpinSR system, designed for fast 3D super resolution imaging and prolonged cell viability in time-lapse experiments. The microscope system offers XY resolution down to 120 nm without the need for dedicated labeling procedures. Learn how to easily integrate the IXplore SpinSR microscope system into existing experiments and sample protocols to streamline your research.

Metabolic Imaging in Langerhans Human Islets with MPE and FLIM

Experts
Francesco Cardarelli
Associate Professor in Applied Physics
Scuola Normale Superiore of Pisa
Capturing life (mis)regulation at the nanoscale is a crucial challenge for present and future biophysics. At this scale, the main actors are the molecules. To successfully tackle molecular behavior within living matter, optical microscopy is a valuable methodological platform: by using fluorescence as the signal, spatial and temporal details of molecular processes can be investigated quantitatively. The physiopathology of beta-cell response to glucose stimulation will be used as case study of biological/biomedical interest. The metabolic traits of beta cells will be highlighted using a straightforward combination of multiphoton microscopy, fluorescence lifetime imaging, and feedback-based orbital tracking of sub-cellular nanostructures.

Accelerating Image Analysis with TruAI™ Deep Learning Technology

Experts
Manoel Veiga
Application Specialist, Life Science Research
Olympus Soft Imaging Solutions
In this tech talk, through a collection of examples measured with our live cell imaging systems, high-content screening station, and whole slide scanner, you will see what TruAI technology can do for your research and get a preview of what is coming next.

Recent Advances in 3D Imaging and AI-Driven Data Analysis

Experts
Seungil Kim
Staff Scientist
Ellison Institute for Transformative Medicine at the University of Southern California
This presentation will highlight various imaging techniques for 3D models, immunostaining with tissue clearing, and live imaging of organoids as well as AI-driven data analysis for high-content imaging and screening.

A Live Demonstration of the SLIDEVIEW™ VS200 Research Slide Scanner

Experts
Chunsong Yan
Business Development Manager, Life Science
Olympus Australia & New Zealand
Tong Wu
Business Development Manager
Olympus Australia New Zealand
Presentation Description: The Olympus SLIDEVIEW VS200 research slide scanner captures high-quality virtual slide images and enables advanced quantitative image analysis. Reliable virtual slide data can be acquired with as few as two clicks. Highly versatile, the SLIDEVIEW VS200 slide scanner supports five observation methods and a wide range of sample sizes for use in various applications. Its automatic slide loader accommodates many slide glasses, helping increase experiment efficiency.

Now You Have the Power to See More

Experts
Sandrine Roy
Business Development Manager
Olympus Australia & New Zealand
The Olympus VS200 digital slide scanner has been very well received since its release in March 2020. With a reliable, flexible, and customizable design, the system has been adopted by various industries including research, geology, and many others. Join us to find out more and see examples of samples scanned using this popular addition to the Olympus product range.

The Use of Multiplexing in Microscopy for Better Understanding the Skin Immune System in the Context of the Tissue

Experts
Alfonso J. Schmidt
Senior Staff Scientist
Malaghan Institute of Medical Research
The skin is the first line of defense and the immune system’s biggest barrier for combating pathogens. Being able to accurately characterize and identify immune cell subtypes, tissues structures, and cell distribution in the skin under steady-state conditions provides a powerful tool for understanding the first immunological strategies and biological processes that occur in the presence of pathogens. In this webinar we will review technical aspects involved in the experimental process and explore how complementary imaging technologies might assist us to better understand the immune system. The presentation is divided into three parts. First, an introduction of the Hugh Green Cytometry Centre will be presented and an overview of the histology and bioimaging technological platforms available. Second, the multiplexing methodology will be discussed, where several topics need to be considered for the design and development of a successful polychromatic panel for microscopy. Finally, preliminary results from a research project will be presented that constitutes part of a diploma program from The Royal Microscopical Society. The project focuses on the identification of immune cell types in the whole mount skin in relation to tissues structures (e.g., blood vessels and lymphatic network). It also centers on the immune cells’ distribution in the tissue as a first barrier of defense against pathogens.

Hyperspectral and Brightfield Imaging Combined with Deep Learning Uncover Hidden Regularities of Colors and Patterns in Cells and Tissues

Experts
Ewa Goldys
Deputy Director
Australian Research Council Centre of Excellence in Nanoscale Biophotonics
The Australian Research Council Centre of Excellence for Nanoscale Biophotonics draws on key advances of the 21st century, nanoscience, and photonics to help understand life at the molecular level. In this presentation, next-generation technologies developed in our Centre for probing, imaging, and interacting with the living systems will be discussed. These address the key challenges of ultrasensitive detection of key analytes in real complex environments and molecular complexity, and they support both novel therapies and diagnostics.

Create a Smarter Cell Culture Workflow

Experts
Joanna Hawryluk
Product Manager
In this webinar, expert Joanna Hawryluk explores how the OLYMPUS Provi™ CM20 incubation monitoring system can help improve the health and stability of cell cultures through machine learning. With the aid of AI, the CM20 monitor automatically measures cell conditions using constant analysis parameters to provide quantitative data—all while your cultures remain safely in the incubator.

Culture and Quantitative 3D Imaging of Organoids: Challenges and Solutions

Experts
Dr. Anne Beghin
Assistant Professor, Research Mechanobiology Institute
National University of Singapore
Turning organoids into impactful translational models includes being able to culture them and assess those that develop robustly with physiologically relevant architecture. However, quantitative comparisons and statistical analysis at high content, which are mandatory to describe the complexity of such multicellular 3D objects are not possible owing to the lack of high-throughput 3D imaging methods. We have thus engineered a versatile High Content Screening (HCS) device to streamline all the steps of organoid culture to exploit its potential in morphogenesis understanding. Our approach comprises a new generation of versatile scaffolding cell culture multiwell chips with embedded optical components (= lighting JeWells) that enables rapid 3D imaging.
Converting from 2D to 3D: Bio-Techne Solutions for Your 3D Culture

Converting from 2D to 3D: Bio-Techne Solutions for Your 3D Culture

Experts
Dr. Xiaotong Cui
Field Application Specialist
Bio-Techne
Organoid and three-dimensional (3D) cell culture are emerging as pivotal systems for understanding human organ development, modeling disease, screening for drug efficacy or toxicity, and investigating personalized medicine. Usually they are derived from primary tissue, embryonic stem cells (ESCs), or induced pluripotent stem cells (iPSCs), which are capable of self-renewal and differentiation.
Utilizing Tumoroids to Explore Anti-Tumor Immunity in Rectal Cancer

Utilizing Tumoroids to Explore Anti-Tumor Immunity in Rectal Cancer

Experts
Dr. Kasmira Wilson
Researcher
Peter MacCallum Cancer Foundation
Globally colorectal cancer is a significant public health burden. It is the third most commonly diagnosed cancer and fourth leading cause of cancer related deaths in the world. A subset of patients diagnosed with rectal cancer require neoadjuvant chemoradiotherapy (NACRT) prior to surgery. However, there is a spectrum of response to this therapy with only 10-20% of patients achieving a complete pathological response. In addition, 20-40% of patients will demonstrate no response to this treatment. There is currently no method that predicts how a patient will respond to NACRT accurately. In order to investigate the mechanisms underpinning how patients respond to therapy, patient derived tumouroids have been utilised. These personalised in vitro three-dimensional tumour models recapitulate the in vivo tumour of origin genotypically. The Ramsay laboratory (Peter MacCallum, Melbourne) has successfully co-cultured patient-matched rectal cancer tumouroids with tumour infiltrating lymphocytes (TILS) in a novel in vitro assay, preliminary data generated suggests this assay has the ability to predict the response of a patient to treatment with NACRT prior to instigation of neo-adjuvant therapy. This assay provides a pre-clinical platform that encapsulates the hosts immune response toward their tumour. However, manual analysis of the data generated from this assay is time consuming and limits the clinical utility of this platform. Machine-based learning to develop artificial neural networks capable of analysing data produced from the killing assay has been developed to automate analysis. Automated analysis utilising artificial neural networks is a feasible approach to expedite the processing of data generated from the cytotoxic killing assays and will improve the clinical utility of this platform to direct personalised patient therapy.
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