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Three-Dimensional High-Throughput Image Analysis of Patient-Derived Organoids and Spheroids

Three-Dimensional High-Throughput Image Analysis of Patient-Derived Organoids and Spheroids

Organoids and spheroids can more faithfully reproduce in vivo conditions than standard 2D cell cultures, and imaging-based analysis can monitor cell-specific responses with high spatial resolution. Therefore, we have been developing imaging-based three-dimensional analysis and drug evaluation methods using patient-derived cancer organoids and spheroids.

In this webinar, we will present some examples of applications within recent trends in organoid research.

Presenters:

Hiroya Ishihara
Applications Scientist Olympus Technologies Asia, Olympus Corporation

FAQ

Webinar FAQs | 3D High-Throughput Image Analysis of PDOs and Spheroids

What sets NoviSight™ software apart from other 3D imaging analysis software?

While other image analysis software may have a graphical user interface and recognize complex 3D structures, NoviSight software is unique for its high-throughput 3D image analysis and intuitive object classification. For instance, NoviSight software is ideal for performing a high-throughput analysis of multiple samples using FRET format.

Can NoviSight software be used for other 3D imaging applications?

In addition to analysis of organoids and spheroids, NoviSight software can perform measurements based on basic object recognition and classifaction for a range of applications. For example, you can quantify the region expressing a specific gene in 3D organs. However, NoviSight software does not have a module for recognizing filament structures, such as dendrites, so it cannot be used to analyze these objects.

Can NoviSight software be used to analyze 2D objects?

Yes, of course. You can measure 2D objects using general object recognition and classification. If you plan to do only 2D analysis, cellSens™ software may be more suitable because it is optimized for 2D analysis. However, if you want to analyze both 2D and 3D objects, NoviSight software is the ideal choice.

Can NoviSight software be used with widefield microscopy?

3D data acquired with the widefield systems is unsuitable for analysis, as images do not have a high enough resolution. We recommend you use cellSens software to employ the deconvolution process.

Can NoviSight software be used for plant tissues?

Yes. With the object recognition and classification algorithms, you can detect the area of specific gene expression.

What is a good clearing agent for plant tissues?

The transparency technique “TOMEI” is suitable for plant analysis.

Is it possible to analyze the immune infiltration into spheroids using live cell imaging without clearing technology?

Analyzing immune infiltration is possible but likely very difficult. We recommend using small 3D models (organoids/spheroids) that are smaller than 100 μm in size. Consider combining this approach with a resonant scanner for high-speed imaging to optimize your results.

Does optical clearing cause tissue shrinkage?

Sometimes the sample shrinks or expands when using a transparent clearing reagent. However, in our experience, the SCALEVIEW-S4 tissue clearing reagent is suitable for organoids and spheroids, and there is no expansion or shrinking.

How much time does it take to count the number of cells in a spheroid?

It takes 10 minutes or less to count the number of cells in a spheroid.

Does NoviSight software include deep-learning technology?

Our TruAI™ deep-learning technology is available in both cellSens software and the scanR high-content screening station but is not available in NoviSight software.

Is a free trial available for NoviSight software?

To try the software for free, contact your local representative to receive a trial version.


Related Products

3D Cell Analysis Software

NoviSight

  • 3D image recognition from a whole structure to sub-cellular
  • Statistical analysis
  • Variety of default assays

Three-Dimensional High-Throughput Image Analysis of Patient-Derived Organoids and Spheroids

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Ask the Experts: 3D High-Throughput Image Analysis Ofpdos and Spheroids

Organoids and spheroids can more faithfully reproduce in vivo conditions than standard 2D cell cultures, and imaging-based analysis can monitor cell-specific responses with high spatial resolution. Therefore, we have been developing imaging-based three-dimensional analysis and drug evaluation methods using patient-derived cancer organoids and spheroids.

In this webinar, we will present some examples of applications within recent trends in organoid research.

FAQ

Webinar FAQs | 3D High-Throughput Image Analysis of PDOs and Spheroids

What sets NoviSight™ software apart from other 3D imaging analysis software?

While other image analysis software may have a graphical user interface and recognize complex 3D structures, NoviSight software is unique for its high-throughput 3D image analysis and intuitive object classification. For instance, NoviSight software is ideal for performing a high-throughput analysis of multiple samples using FRET format.

Can NoviSight software be used for other 3D imaging applications?

In addition to analysis of organoids and spheroids, NoviSight software can perform measurements based on basic object recognition and classifaction for a range of applications. For example, you can quantify the region expressing a specific gene in 3D organs. However, NoviSight software does not have a module for recognizing filament structures, such as dendrites, so it cannot be used to analyze these objects.

Can NoviSight software be used to analyze 2D objects?

Yes, of course. You can measure 2D objects using general object recognition and classification. If you plan to do only 2D analysis, cellSens™ software may be more suitable because it is optimized for 2D analysis. However, if you want to analyze both 2D and 3D objects, NoviSight software is the ideal choice.

Can NoviSight software be used with widefield microscopy?

3D data acquired with the widefield systems is unsuitable for analysis, as images do not have a high enough resolution. We recommend you use cellSens software to employ the deconvolution process.

Can NoviSight software be used for plant tissues?

Yes. With the object recognition and classification algorithms, you can detect the area of specific gene expression.

What is a good clearing agent for plant tissues?

The transparency technique “TOMEI” is suitable for plant analysis.

Is it possible to analyze the immune infiltration into spheroids using live cell imaging without clearing technology?

Analyzing immune infiltration is possible but likely very difficult. We recommend using small 3D models (organoids/spheroids) that are smaller than 100 μm in size. Consider combining this approach with a resonant scanner for high-speed imaging to optimize your results.

Does optical clearing cause tissue shrinkage?

Sometimes the sample shrinks or expands when using a transparent clearing reagent. However, in our experience, the SCALEVIEW-S4 tissue clearing reagent is suitable for organoids and spheroids, and there is no expansion or shrinking.

How much time does it take to count the number of cells in a spheroid?

It takes 10 minutes or less to count the number of cells in a spheroid.

Does NoviSight software include deep-learning technology?

Our TruAI™ deep-learning technology is available in both cellSens software and the scanR high-content screening station but is not available in NoviSight software.

Is a free trial available for NoviSight software?

To try the software for free, contact your local representative to receive a trial version.


Related Products

3D Cell Analysis Software

NoviSight

  • 3D image recognition from a whole structure to sub-cellular
  • Statistical analysis
  • Variety of default assays
Experts
Hiroya Ishihara
Applications Scientist, Olympus Technologies Asia, Olympus Corporation

Hello. My name is Hiroya Ishihara, and I'm an application scientist at Olympus. At the Tokyo University of Science, I studied epigenetic factors involved in plant regeneration using omics and microscopy. Confocal and two-photon microscopes were my trusted partners at that time. I joined Olympus to help advance life science research and make it more exciting with microscopy. Currently, I'm working on a wide range of projects from basic research to product/sales strategy.

Three-Dimensional High-Throughput Image Analysis of Patient-Derived Organoids and SpheroidsSep 22 2021
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