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Confocal Laser Scanning Imaging
A Line Objectives and the FV3000 Microscope/IXplore

Silicone Immersion Objectives

Silicone immersion objectives are optimized for live cell and live tissue imaging. By properly matching the refractive index, images are clearer and brighter, and time−lapse observations become more reliable and less complex because silicone oil does not dry at 37 °C (98.6 ℉). Because the refractive index of silicone immersion oil (ne=1.40) is close to that of the clearing reagent SCALEVIEW–A2 (ne=1.38), the silicone immersion objectives are also well suited for observing SCALEVIEW–A2 cleared samples.

Silicone immersion objectives

Image data courtesy of Motokazu Uchigashima, M.D., Ph.D., Masahiko Watanabe, M.D., Ph.D., Departments of Anatomy, Hokkaido University Graduate School of Medicine
Sample: ScaleA2–treated neocortex, VGluT1/Green, VGluT2/Red, MAP2/Blue

Silicone Versus Oil Immersion 60X Objectives

By matching the refractive index of the sample and immersion medium, the A Line silicone objective (UPLSAPO60XS2) enables deeper imaging.
 

Image data courtesy of Motokazu Uchigashima, M.D., Ph.D., Masahiko Watanabe, M.D., Ph.D.,
Departments of Anatomy, Hokkaido University Graduate School of Medicine

Learn more about live cell imaging applications

Three-Dimensional Observation of the Biliary Tree Structure in Mouse Livers with a 30x Objective (UPLSAPO30XS) 

The biliary tree structures were compared between Klf5-LKO mice and control mice. Consecutive tomographic images (Z axial interval of 1 μm) of biliary tissue (green, biliary epithelial cell marker CK19) in liver tissue specimens with 200 μm thickness cleared with SeeDB were obtained using the FV3000 and 30x silicone oil immersion objective, enabling high-resolution observation while maintaining a wide field of view. In Klf5-LKO mice, CK19+ cell clusters (white arrow) that were spatially separated from the biliary tree were observed.

Controlled Mouse
KIf5-LKO mouse

Scale bar: 50 μm

Silicone Immersion Objectives Selection Guide

Working Distance
(mm)
Magnification Objective Field Number* Numerical Aperture Immersion Applications
UPLSAPO100XS 0.2 100X 22 1.35 Silicone oil High-resolution for subcellular imaging
UPLSAPO60XS2 0.3 60X 22 1.30 Silicone oil High-resolution, long-term, time-lapse imaging of single cells
UPLSAPO40XS 0.3 40X 22 1.25 Silicone oil Multiple cell imaging with a wider field of view
UPLSAPO30XS 0.8 30X 22 1.05 Silicone oil Deeper tissue imaging with a wider field of view

*Maximum field number observable through eyepiece.

Super-Corrected 60X Objectives

PLAPON60XOSC2
UPLXAPO60XO (left) versus PLAPON60XOSC2 (right)

Cyan: 405 nm excitation, Magenta: 640 nm excitation

Comparison of chromatic aberration measured by FLUOVIEW FV3000 using multicolor fluorescent microshpere.

Better Imaging Performance with a Super-Corrected Chromatic Aberration and a High Numerical Aperture

Colocalized fluorescent signals are a problem that require an objective with a superior optical design to correct for color shifts (aberration). The high numerical aperture super-corrected 60X objective minimizes chromatic aberration to the utmost limit in the 405–650 nm spectrum.  0.1 μm or less axial chromatic aberration is provided in this range, and every objective is delivered with its measured data sheet. This objective can acquire 2D and 3D images with excellent reliability, accuracy, and improved colocalization analysis.  Save time and resources in multicolor labeling experiments without having to go through post-processing adjustments.

Super-Corrected 60X Objectives Specifications

Working Distance
(mm)
Magnification Objective Field Number* Numerical Aperture Immersion
PLAPON60XOSC2 0.12 60X 22 1.4 Oil

*Maximum field number observable through eyepiece.

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  • Accurate 3D reconstruction with Olympus silicone oil immersion objectives

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*Banner Image: By courtesy of 
Division of Mammalian Development, Genetic Strains Research Center, National Institute of Genetics, Dr. Hajime Okada
Laboratory of Stem Cell Therapy, Institute for Quantitative Biosciences, The University of Tokyo, Project Associate Professor, Dr. Tohru Itoh

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