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Live Cell Imaging
Silicone Immersion Objectives and the FV3000/ IXplore

Silicone Immersion Objectives

Silicone immersion objectives are optimized for live cell and live tissue imaging. With a refractive index similar to that of living tissue, silicone immersion objectives offer bright images of cleared tissue. And because silicone oil doesn’t dry out at 37 °C (98.6 °F), time−lapse observations are more reliable and less complex.

livecell_objectives

Effects of Refractive Index Mismatch on Sample Shape

Effects of Refractive Index Mismatch on Sample Shape

Matching the refractive index of a sample and immersion media is very important to get accurate 3D images.

The Brightness of Water/Silicone/Oil 60X Objectives       

Oil immersion objectives are the brightest at superficial depths while silicone immersion objectives are brighter than water and oil immersion objectives at all other focus depths.
The brightness of each objective is normalized to the 60X oil objective at the sample’s surface. The sample's refractive index is 1.38.

Comparing the Brightness of 60X Objectives

Long–Term, Time–Lapse Imaging of a Live Mouse Embryo (UPLSAPO60XS2)

Long-term, time-lapse images of a live mouse embryo captured using a silicone immersion objective (UPLSAPO60XS2). 
The objective enables high‒resolution imaging with a numerical aperture (NA) of 1.30 and 3D imaging with a 0.3 mm working distance.

Image data courtesy of Kazuo Yamagata Ph.D., Faculty of Biology–Oriented Science and Technology, Kinki University Reference Stem: Cell Reports. 2014 Jun 3; 2 (6): 910–924.

Learn more about live cell imaging applications

Long–Term, Live–Cell Imaging of Arabidopsis Zygote Embryogenesis (UPLSAPO30XS)

The UPLSAPO30XS objective has an NA of 1.05 for brighter images with a higher resolution.

The video shows the process of embryogenesis from the early embryo (4–cell stage) to the late embryo recorded over 60 hours.

Image data courtesy of Daisuke Kurihara, Ph.D., Optical Technology Group, ERATO Higashiyama Live–Holonics Project, Nagoya University
Reference: Dev Cell. 2015 Jul 27; 34 (2): 242–51. doi: 10.1016/j.devcel.2015.06.008. Epub 2015 Jul 9.

Learn More About Live Cell Imaging Applications

Silicone Immersion Objectives Selection Guide

Working Distance
(mm)
Magnification Objective Field Number* Numerical Aperture Immersion Applications
UPLSAPO100XS 0.2 100X 22 1.35 Silicone oil High-resolution for subcellular imaging
UPLSAPO60XS2 0.3 60X 22 1.30 Silicone oil High-resolution and long-term, time-lapse imaging of single cells
UPLSAPO40XS 0.3 40X 22 1.25 Silicone oil Multiple cell imaging with a wider field of view
UPLSAPO30XS 0.8 30X 22 1.05 Silicone oil Deeper tissue imaging with a wider field of view

*Maximum field number observable through eyepiece.

Related Products

FV3000

FV3000

  • Available with galvanometer-only (FV3000) or hybrid galvanometer/resonant (FV3000RS) scanner configurations 
  • Efficient, accurate TruSpectral detection on all channels 
  • Optimized for live-cell imaging with high sensitivity and low phototoxicity

Learn More

IXplore Live

IXplore Live

  • Utilize the Olympus real-time controller for physiologically relevant data with minimal cell disturbance 
  • Maintain cell viability while imaging with various environmental control options 
  • Maintain focus accurately and reliably in time-lapse experiments with the Olympus hardware autofocus (Z-drift compensation) system 
  • Discover the real shape of your cells with Olympus silicone immersion optics 

Learn More

IXplore Spin

IXplore Spin

  • Rapid and high-resolution confocal imaging with a spinning disk system
  • 3D confocal time-lapse imaging of live cells with less phototoxicity and bleaching
  • Precise 3D imaging with improved light collection using silicone oil immersion objectives
  • Upgrade to the IXplore SpinSR super resolution system depending on your research progress and/or budget

Learn More

IXplore SpinSR

IXplore SpinSR

  • Super resolution down to 120 nm XY resolution 
  • Prolonged cell viability in confocal time-lapse imaging due to less phototoxicity and bleaching 
  • Switch between widefield, confocal, and super resolution observations in the IXplore SpinSR system in one step
  • Accurate 3D reconstruction with Olympus silicone oil immersion objectives

Learn More

*Banner Image: By courtesy of Dr. Kazuo Yamagata, Dr. Mayuko Hori, Dr. Tatsuma Yao, Research Institute for Microbial Disease, Osaka University

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