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Deconvolution in Optical Microscopy

Section Overview

Deconvolution is an image processing technique used to improve the contrast and resolution of images captured using an optical microscope. Out of focus light causes blur in a digital image. Mathetically, this can be represented as a convolution operation. Deconvolution seeks to remove or reassign this out of focus light present in digital images, thus improving the resolution of the final micrograph. Nearly all fluoresence images can be deconvolved, with advanced techniques like confocal and super-resolution also realizing the benefits of deconvolution.

Left: Without TruSight / Right: With TruSight
Left: Without TruSight / Right: With TruSight

Review Articles

Selected Literature References and Internet Resources

  • Deconvolution Resources

    Listed in this section are links to web resources on deconvolution analysis, including software packages, hardware (microscopes and accessories), and laboratories involved with the technology.

  • Literature References

    A number of review articles on deconvolution in optical microscopy have been published by researchers in the field and were utilized as references for this section.

Contributing Authors

Wes Wallace - Department of Neuroscience, Brown University, Providence, Rhode Island 02912.

Lutz H. Schaefer - Advanced Imaging Methodology Consultation, Kitchener, Ontario, Canada.

Jason R. Swedlow - Division of Gene Regulation and Expression, School of Life Sciences Research, University of Dundee, Dundee, DD1 EH5 Scotland.

Thomas J. Fellers and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.

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