Life Science Solutions

Fluorochrome Data Tables

Our visitors will find in the following listing many of the commonly-used fluorochromes, with their respective peak excitation and emission wavelengths.

While the authors assume responsibility for the accurate reporting of the data as published in various reliable sources, several caveats must be given. Fluorochrome dyes are environmentally sensitive and different results will be obtained with different solvents and applications. In reviewing the literature, you will frequently find somewhat different data supplied for the identical fluorochrome. There are also, in many instances, several sub-varieties of a fluorochrome.

Fluorescence Filter Cubes

Discover how variations in excitation and barrier filters allow certain wavelengths to pass.

Much information about fluorochromes will be found in sources cited in the bibliography and Web resources guide which visitors will find on the primer introductory page. See particularly the books edited by Taylor et al. and the literature published by microscope companies.

It is strongly urged that the data for a particular fluorochrome be confirmed by consulting the dye manufacturer to secure full specification of curves for excitation and emission.

Fluorochrome Peak Excitation and Emission Wavelengths
Fluorochrome Excitation Wavelength Emission Wavelength
Acid Fuchsin 540 630
Acridine Orange
(Bound to DNA)
502 526
Acridine Red 455-600 560-680
Acridine Yellow 470 550
Acriflavin 436 520
AFA (Acriflavin
Feulgen SITSA)
355-425 460
Alizarin Complexon 530-560 580
Alizarin Red 530-560 580
Allophycocyanin 650 661
ACMA 430 474
AMCA-S, AMC 345 445
Aminoactinomycin D 555 655
7-Aminoactinomycin D-AAD 546 647
Aminocoumarin 350 445
Anthroyl Stearate 361-381 446
Astrazon Brilliant Red 4G 500 585
Astrazon Orange R 470 540
Astrazon Red 6B 520 595
Astrazon Yellow 7 GLL 450 480
Atabrine 436 490
Auramine 460 550
Aurophosphine 450-490 515
Aurophosphine G 450 580
BAO 9-(Bisamino-phenyloxadiazole) 365 395
BCECF 505 530
Berberine Sulphate 430 550
Bisbenzamide 360 600-610
BOBO-1, BO-PRO-1 462 481
Blancophor FFG Solution 390 470
Blancophor SV 370 435
Bodipy Fl 503 512
Bodipy TMR 542 574
Bodipy TR 589 617
BOPRO 1 462 481
Brilliant Sulpho-flavin FF 430 520
Calcein 494 517
Calcien Blue 370 435
Calcium Green 505 532
Calcium Orange 549 576
Calcofluor RW Solution 370 440
Calcofluor White 440 500-520
Calcofluor White ABT Solution 380 475
Calcofluor White Standard Solution 365 435
5-(and 6-)carboxy SNARF-1 indicator 548(low pH)
576(high pH)
587(low pH)
635(high pH)
6-Carboxyrhodamine 6G 525 555
Cascade Blue 400 425
Catecholamine 410 470
Chinacrine 450-490 515
CL-NERF 504(low pH)
514(high pH)
587(low pH)
540(high pH)
Coriphosphine O 460 575
Coumarin-Phalloidin 387 470
CY3.18 554 568
CY5.18 649 666
CY7 710 805
DANS (1-DimethylAmino-Naphthaline-5-Sulphonic Acid) 340 525
DANSA (DiaminoNaphthyl-Sulphonic Acid) 340-380 430
Dansyl NH-CH3 in water 340 578
DAPI 350 470
DiA 456 590
Diamino Phenyl Oxydiazole (DAO) 280 460
Di-8-ANEPPS 488 605
Dimethylamino-5-Sulphonic Acid 310-370 520
DiI [DiIC18(3)] 549 565
DiO [DiOC18(3)] 484 501
Diphenyl Brilliant Flavine 7GFF 430 520
DM-NERF 497(low pH)
510(high pH)
527(low pH)
536(high pH)
Dopamine 340 490-520
ELF-97 alcohol 345 530
Eosin 525 545
Erythrosin ITC 530 558
Ethidium Bromide 510 595
Euchrysin 430 540
FIF (Formaldehyde Induced Fluorescence) 405 435
Flazo Orange 375-530 612
Fluorescein 494 518
Fluorescein Iso-thiocyanate (FITC) 490 525
Fluo 3 485 503
FM1-43 479 598
Fura-2 363(low [Ca2+])
335(high [Ca2+])
512(low [Ca2+])
505(high [Ca2+])
Fura Red 472(low [Ca2+])
436(high [Ca2+])
657(low [Ca2+])
637(high [Ca2+])
Genacryl Brilliant Red B 520 590
Genacryl Brilliant Yellow 10GF 430 485
Genacryl Pink 3G 470 583
Genacryl Yellow 5GF 430 475
Gloxalic Acid 405 460
Granular Blue 355 425
Haematoporphyrin 530-560 580
Hoechst 33258, 33342 (Bound to DNA) 352 461
3-Hydroxypyrene-5,-8,10-TriSulfonic Acid 403 513
7-Hydroxy-4-methylcoumarin 360 455
5-Hydroxy-Tryptamine (5-HT) 380-415 520-530
Indo-1 350 405-482
Intrawhite Cf Liquid 360 430
Leucophor PAF 370 430
Leucophor SF 380 465
Leucophor WS 395 465
Lissamine Rhodamine B200 (RD200) 575 595
Lucifer Yellow CH 425 528
Lucifer Yellow VS 430 535
LysoSensor Blue DND-192, DND-167 374 425
LysoSensor Green DND-153, DND-189 442 505
LysoSensor Yellow/Blue 384(low pH)
329(high pH)
540(low pH)
440(high pH)
LysoTracker Green 504 511
LysoTracker Yellow 534 551
LysoTracker Red 577 592
Magdala Red 524 600
Magnesium Green 506 531
Magnesium Orange 550 575
Maxilon Brilliant Flavin 10 GFF 450 495
Maxilon Brilliant Flavin 8 GFF 460 495
Mitotracker Green FM 490 516
Mitotracker Orange CMTMRos 551 576
MPS (Methyl Green Pyronine Stilbene) 364 395
Mithramycin 450 570
NBD 465 535
NBD Amine 450 530
Nile Red 515-530 525-605
Nitrobenzoxadidole 460-470 510-650
Noradrenaline 340 490-520
Nuclear Fast Red 289-530 580
Nuclear Yellow 365 495
Nylosan Brilliant Flavin E8G 460 510
Oregon Green 488 fluorophore 496 524
Oregon Green 500 fluorophore 503 522
Oregon Green 514 fluorophore 511 530
Pararosaniline (Feulgen) 570 625
Phorwite AR Solution 360 430
Phorwite BKL 370 430
Phorwite Rev 380 430
Phorwite RPA 375 430
Phosphine 3R 465 565
Phosphine R 480-565 578
Pontochrome Blue Black 535-553 605
POPO-1, PO-PRO-1 434 456
Primuline 410 550
Procion Yellow 470 600
Propidium Iodide 536 617
Pyronine 410 540
Pyronine B 540-590 560-650
Pyrozal Brilliant Flavin 7GF 365 495
Quinacrine Mustard 423 503
R-phycoerythrin 565 575
Rhodamine 110 496 520
Rhodamine 123 511 534
Rhodamine 5 GLD 470 565
Rhodamine 6G 526 555
Rhodamine B 540 625
Rhodamine B 200 523-557 595
Rhodamine B Extra 550 605
Rhodamine BB 540 580
Rhodamine BG 540 572
Rhodamine Green fluorophore 502 527
Rhodamine Red 570 590
Rhodamine WT 530 555
Rhodol Green fluorophore 499 525
Rose Bengal 540 550-600
Serotonin 365 520-540
Sevron Brilliant Red 2B 520 595
Sevron Brilliant Red 4G 500 583
Sevron Brilliant Red B 530 590
Sevron Orange 440 530
Sevron Yellow L 430 490
SITS (Primuline) 395-425 450
SITS (Stilbene Isothiosulphonic Acid) 365 460
Sodium Green 507 535
Stilbene 335 440
Snarf 1 563 639
Sulpho Rhodamine B Can C 520 595
Sulpho Rhodamine G Extra 470 570
SYTOX Green nucleic acid stain 504 523
SYTO Green fluorescent nucleic acid stains 494 ± 6 515 ± 7
SYTO Green fluorescent nucleic acid stains 515 ± 7 543 ± 13
SYTO 17 red fluorescent nucleic acid stain 621 634
Tetracycline 390 560
TRITC (Tetramethyl Rhodamine Isothiocyanate) 557 576
Texas Red 596 615
Thiazine Red R 510 580
Thioflavin S 430 550
Thioflavin TCN 350 460
Thioflavin 5 430 550
Thiolyte 370-385 477-484
Thiozol Orange 453 480
Tinopol CBS 390 430
TOTO 1, TO-RRO-1 514 533
TOTO 3, TO-PRO-3 642 661
True Blue 365 420-430
Ultralite 656 678
Uranine B 420 520
Uvitex SFC 365 435
X-Rhodamine 580 605
Xylene Orange 546 580
XRITC 582 601
YOYO-1, YOYO-PRO-1 491 509
YOYO-3, YOYO-PRO-3 612 631

Contributing Authors

Mortimer Abramowitz - Olympus America, Inc., Two Corporate Center Drive., Melville, New York, 11747.

Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.

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