Life Science Solutions

Fluorochrome Data Tables

The data tables below contain reference information on fluorescence cubes offered by Olympus America, Inc. to support their microscopes. The codes for the excitation columns in Tables 2 and 4 and the cube code column in Table 3 are listed in Table 1. Table 2 is a listing of examples of cubes provided for U-URA microscopes. The authors wish to caution that we believe this data to be accurate, but it should always be verified by authorized Olympus representatives.

Filter Color Codes
CodeFilter Type
UUltraviolet
VViolet
BVBlue-Violet
BBlue
IBInterference
Blue Filter
GGreen
IGImmuno Gold
Staining Method
BFBrightfield,
Phase Contrast,
and DIC only
Table 1

Table 2 lists the excitation and barrier filters by cube along with the dichroic mirror used in each cube. Use this chart as an aid to guide you in selection of some of the Olympus U-URA cubes for applications listed in our fluorescence and fluorochrome data tables.

Fluorescence Filter Cubes

Discover how variations in excitation and barrier filters allow certain wavelengths to pass.

Olympus Cubes Offered for U-URA
ExcitationCubeExcitation Filter(nm)Dichroic Mirror(nm)Barrier Filter
UU-MWU330-385400420--
UU-MNU360-370400420--
UU-MNUBP360-370400420-460
VU-MNV400-410455455--
BVU-MWBV400-440455475--
BVU-MNBV420-440455475--
BU-MWB450-480500515--
IBU-MWIB460-490505515IF--
IBU-MNIB470-490505515IF--
IBU-MNIBBP470-490505515-550
IBU-MWIBBP460-490505515-550
GU-MSWG480-550570590--
GU-MWG510-550570590--
GU-MNG530-550570590--
IGU-MNIG520-550565580IF--
IYU-MWIY545-580600610IF--
Table 2

Table 3 lists dichroic mirror/filter combinations for the Olympus BH-2 and Vanox-3 fluorescent illuminators. The authors believe this information to be correct but it should be verified by Olympus representatives. Refer to Table 1 for the codes in the first column of this table.

CODECubeDichroic MirrorExciter
Filter
Barrier
Filter
Supplementary Exciter
Filter
Additional Barrier
Filter
U-DMUDM400UG1L420L435
Y455
V-DMVDM455BP405Y455 Y495
Y495
515
BV-DMBVDM455BP440AFCEL420Y495
+Y475515
B-DMBDM500BP490AFCEL455B460
+O515530
570
590
G520
IB-DMIBDM505BP4950515IFEY475B460
530
570
590
G-DMGDM570BP545590EO515R610
EO530
IGS-HMIGS(Half-Mirror)L420
+PO
(Polarizer)
AN + DP
BF-BF(Optical
axis
adjuster)
(Light
beam
shutter)
(Image
position
adjuster)
----------
Table 3

Table 4 is a cross-reference for Olympus U-URA cubes to Olympus BH-2, Vanox-3, and IMT-2 cubes. It is advisable to consult the manufacturer's wavelength/transmission curve diagrams for exciter filters, dichroic mirrors and barrier filters to determine how they match the fluorochrome being used. The cube names and nanometer numbers refer to the Olympus U-URA cubes; the figures in parentheses refer to filters still current for BH-2, Vanox 3, and IMT-2 fluorescent cubes.

ExcitationCubeExcitation FilterDichroic
Mirror
Barrier
Filter
UU-MUBP330-385DM400BA420
(UG-1)(L420)
VU-MNVBP400-410DM455BA455
(BP405)(Y455)
BVU-MWBVBP400-440DM455Ba475
(BP440)(Y475&AFC)
BVU-MNBVBP420-440DM455BA475
(BP440&EL420)(Y475&AFC)
BU-MWBBP450-480DM500BA515
(BP490&EY455)(O515&AFC)
IBU-MWIBBP460-490DM505BA515IF
(BP495)(O515IF)
IBU-MNIBBP470-490DM505BA515IF
(BP495&EY455)(O515IF)
GU-MSWGBP480-550DM570BA590
(BP545)(O590)
GU-MWGBP510-550DM570BA590
(BP545&EO515)(O590)
GU-MNGBP530-550DM570BA590
(BP545&EO530)(O590)
Table 4

Use this chart as an aid to guide you in selection of some of the Olympus U-URA cubes if you wish to cross-reference to the extensive listing of fluorochromes in our other data sections.

Contributing Authors

Mortimer Abramowitz - Olympus America, Inc., Two Corporate Center Drive., Melville, New York, 11747.

Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.

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