Guidance for Quantitative Confocal Microscopy

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Guidance for Quantitative Confocal Microscopy

When used correctly, a confocal fluorescence microscope is an excellent tool for making quantitative measurements in cells and tissues. Its ability to block out-of-focus light and thereby perform optical sectioning through a specimen enables you to quantify fluorescence with very high spatial precision.

However, generating meaningful data using confocal microscopy requires careful planning and a thorough understanding of the technique. This webinar will guide you through key aspects of acquiring quantitative confocal microscopy images, including:

• Optimizing sample preparation
• Choosing the most suitable microscope for a given application
• Configuring the microscope parameters

You’ll also learn about common pitfalls such as photobleaching and crosstalk, as well as several instrument problems that may prevent the acquisition of quantitative data.

The webinar is based on a recent Nature Protocols tutorial paper authored by an international group of core microscopy facility directors: James Jonkman, Claire M. Brown, Graham D. Wright, Kurt I. Anderson, and Alison J. North. Tutorial: Guidance for Quantitative Confocal Microscopy. Nature Protocols. (2020) 15: 1585-1611.

Presenters:

Dr. Graham Wright, Chief Technology Officer A*STAR’s Research Support Centre (RSC) Graham holds an interdisciplinary Ph.D. in cell biology and physics from The University of Edinburgh and an MBA from the Warwick Business School at The University of Warwick. He is the Chief Technology Officer of A*STAR’s Research Support Centre (RSC) and the Director of the A*STAR Microscopy Platform (AMP). As CTO, Graham is responsible for oversight and coordination of a comprehensive range of core technology platforms across the Biomedical Research Council of A*STAR.

Guidance for Quantitative Confocal Microscopy When used correctly, a confocal fluorescence microscope is an excellent tool for making quantitative measurements in cells and tissues. Its ability to block out-of-focus light and thereby perform optical sectioning through a specimen enables you to quantify fluorescence with very high spatial precision. However, generating meaningful data using confocal microscopy requires careful planning and a thorough understanding of the technique. This webinar will guide you through key aspects of acquiring quantitative confocal microscopy images, including: Optimizing sample preparation Choosing the most suitable microscope for a given application Configuring the microscope parameters You’ll also learn about common pitfalls such as photobleaching and crosstalk, as well as several instrument problems that may prevent the acquisition of quantitative data. The webinar is based on a recent Nature Protocols tutorial paper authored by an international group of core microscopy facility directors: James Jonkman, Claire M. Brown, Graham D. Wright, Kurt I. Anderson, and Alison J. North. Tutorial: Guidance for Quantitative Confocal Microscopy. Nature Protocols. (2020) 15: 1585-1611. Presenters: Dr. Graham Wright, Chief Technology Officer A*STAR’s Research Support Centre (RSC) Graham holds an interdisciplinary Ph.D. in cell biology and physics from The University of Edinburgh and an MBA from the Warwick Business School at The University of Warwick. He is the Chief Technology Officer of A*STAR’s Research Support Centre (RSC) and the Director of the A*STAR Microscopy Platform (AMP). As CTO, Graham is responsible for oversight and coordination of a comprehensive range of core technology platforms across the Biomedical Research Council of A*STAR. Fill out the form to access the webinar Choose ...AfghanistanAlbaniaAlgeriaAmerican SamoaAndorraAngolaAnguillaAntarcticaAntigua and BarbudaArgentinaArmeniaArubaAustraliaAustriaAzerbaijanBahamasBahrainBangladeshBarbadosBelarusBelgiumBelizeBeninBermudaBhutanBoliviaBosnia and HerzegovinaBotswanaBrazilBritish Virgin IslandsBrunei DarussalamBulgariaBurkina FasoBurundiCambodiaCameroonCanadaCape VerdeCayman IslandsCentral African RepublicChadChileChinaChristmas IslandCocos IslandsColombiaComorosCongoCongo, Democratic Republic of the Cook IslandsCosta RicaCote d'IvoireCroatiaCubaCyprusCzech RepublicDenmarkDjiboutiDominicaDominican RepublicEcuadorEgyptEl SalvadorEquatorial GuineaEritreaEstoniaEthiopiaFalkland Islands (Malvinas)Faroe IslandsFijiFinlandFranceFrench GuianaFrench PolynesiaGabonGambiaGeorgiaGermanyGhanaGibraltarGreeceGreenlandGrenadaGuadeloupeGuamGuatemalaGuernseyGuineaGuinea-BissauGuyanaHaitiHeard Island and Mcdonald IslandsHoly SeeHondurasHong KongHungaryIcelandIndiaIndonesiaIranIraqIrelandIsle of ManIsraelItalyJamaicaJapanJerseyJordanKazakhstanKenyaKiribatiKorea, Dem. People's Rep. of Korea, Republic of KuwaitKyrgyzstanLao People's Dem. Rep.LatviaLebanonLesothoLiberiaLibyan Arab JamahiriyaLiechtensteinLithuaniaLuxembourgMacaoMacedoniaMadagascarMalawiMalaysiaMaldivesMaliMaltaMarshall IslandsMartiniqueMauritaniaMauritiusMayotteMexicoMicronesia, Federated States ofMoldova, Republic of MonacoMongoliaMontenegroMontserratMoroccoMozambiqueMyanmarNamibiaNauruNepalNetherlandsNetherlands AntillesNew CaledoniaNew ZealandNicaraguaNigerNigeriaNiueNorfolk IslandNorthern Mariana IslandsNorwayOmanPakistanPalauPalestinePanamaPapua New GuineaParaguayPeruPhilippinesPitcairnPolandPortugalPuerto RicoQatarReunionRomaniaRussian FederationRwandaSaint BarthelemySaint HelenaSaint Kitts and NevisSaint LuciaSaint MartinSaint Pierre and MiquelonSaint Vincent and the GrenadinesSamoaSan MarinoSao Tome and PrincipeSaudi ArabiaSenegalSerbiaSeychellesSierra LeoneSingaporeSlovakiaSloveniaSolomon IslandsSomaliaSouth AfricaSpainSri LankaSudanSurinameSvalbard and Jan Mayen IslandsSwazilandSwedenSwitzerlandSyrian Arab RepublicTaiwanTajikistanTanzania, United Republic of ThailandTimor-LesteTogoTokelauTongaTrinidad and TobagoTunisiaTurkeyTurkmenistanTurks and Caicos IslandsTuvaluU.S. Virgin IslandsUSAUgandaUkraineUnited Arab EmiratesUnited KingdomUruguayUzbekistanVanuatuVenezuelaViet NamWallis and Futuna IslandsWestern SaharaYemenZambiaZimbabweLocation * Company Name * First Name * Last Name * E-mail * City ZIP * Subscribe to the Olympus Life Science Solutions E-newsletter Please choose division Academic ResearchBiotechClinicalEducationDrug DiscoveryRegenerative MedicineOEM ComponentsOthers I agree to the Terms of Use and acknowledge that I have read the Privacy Notice* Submit If you already have your personal ID enter it here: