When used correctly, a confocal fluorescence microscope is an excellent tool for making quantitative measurements in cells and tissues. Its ability to block out-of-focus light and thereby perform optical sectioning through a specimen enables you to quantify fluorescence with very high spatial precision.
However, generating meaningful data using confocal microscopy requires careful planning and a thorough understanding of the technique. This webinar will guide you through key aspects of acquiring quantitative confocal microscopy images, including:
You’ll also learn about common pitfalls such as photobleaching and crosstalk, as well as several instrument problems that may prevent the acquisition of quantitative data.
The webinar is based on a recent Nature Protocols tutorial paper authored by an international group of core microscopy facility directors: James Jonkman, Claire M. Brown, Graham D. Wright, Kurt I. Anderson, and Alison J. North. Tutorial: Guidance for Quantitative Confocal Microscopy. Nature Protocols. (2020) 15: 1585-1611.
![]() | Dr. Graham Wright, Chief Technology Officer |
Guidance for Quantitative Confocal Microscopy
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