Fast, Accurate Cell Counting Streamlines the Cell Culture Process
The cell counter’s software algorithm quickly provides accurate counting results for a variety of cell types by automatically de-clustering clumped cells, performing size- and roundness-based sorting, and identifying live/dead cells. The R1 cell counter can confirm cell counts prior to experimental data acquisition, quality check, passaging, or induction of differentiation. Select from default protocol settings for 18 of the 22 major cell types or customize a protocol to your specific cell type of interest.
Accurately count clustered cells: The counter identifies single, clustered, live, and dead cells for counts with an accuracy similar to a hemocytometer.
Exclude undesired particles with cell-size-based gates: Set the maximum and minimum cell sizes to filter for a given cell type.
Accurately count small cells: The Live Cell Sensitivity function optimizes the recognition rate for cells less than 8 μm.
Automated dilution calculator: Enter your desired concentrations, and the volume of cell suspension needed is automatically calculated.
No trypan blue staining required: The Cell Counter can quickly count and present the total number of cells in a sample that has not been treated with trypan blue. The number of live cells can be estimated when the proportion of live/dead cells in the sample is known.
Trypan blue concentration correction: The Auto Exposure Mode automatically corrects for variations in trypan blue concentration for stable count results.
Clear Cell Images with Liquid Lens Autofocusing
The Cell Counter model R1 automatically focuses on cells using liquid lens technology, which works similar to how human eyes focus. The lens can autofocus in the Z-axis at the right position, producing more accurate and reproducible cell counting results.
Efficient Cell Culture Data Management
Easy Data File Sharing
Store up to 1,000 counts
Easily export your data as a CSV file
Result and image data can be stored as a TIFF, an annotated TIFF, or a PDF report file
Easily transfer data using the cell counter’s USB ports
Comprehensive Report Results at a Glance
No need to manually record detailed settings for each count
Easily obtain report files containing the protocol used, the cell count results, the corresponding raw/analyzed cell images, and histograms
Use review mode to view various reports on the touch screen at the same time, including the number of live/dead cells and average cell size
Custom Cell Counting Parameters
Each detection parameter can be modified to fit the sample conditions. Up to 300 individual cell counting protocols can be saved and reloaded for use in routine procedures.
User-Friendly Cell Counter Design
Portable with a Small Footprint
The handle on top of the counter makes it easy to transport, while its small footprint enables it to fit in tight spaces in your lab. A storage space in the back of the counter hides the AC adaptor and extension cord.
Convenient Touch Screen
The large touch screen at the base of the cell counter offers stability while making it simple to count cells, view data, and print the results.
Easy-to-Load Slides
It’s easy to add cells to the cell counter’s disposable slides through the loading slits. Two sets of cell counting are performed on one slide, making it easy to replicate runs.
195 mm × 237 mm × 272 mm (7.7 in. × 9.3 in. × 10.7 in.)
Weight
2.1 kg (4.6 lb) (without the external power adaptor)
Input Power
AC 100–240 V, 50/60 Hz, 1.2 A
*1: Cell counting at less than 1 × 106 cells/ml concentration of HeLa or HL-60 cells.
*2: Live/dead cell concentration, number, and cell viability can be available with trypan blue mode.
FF hiPS：Feeder-free Human iPS cells (201B7 cell line)
Protocol
Results
Noise reduction: 6
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium
Total cell conc. (cells/ml): 1.50 x 106
Live cell conc. (cells/ml): 1.38 x 106
Dead cell conc. (cells/ml): 1.19 x 105
Viability (%): 92.0
Average cell size (μm): 15.9
Total cell conc. (cells/ml): 1.03 x 106
Live cell conc. (cells/ml): 9.37 x 105
Dead cell conc. (cells/ml): 9.72 x 104
Viability (%): 90.6
Average cell size (μm): 19.6
A549： Human lung carcinoma
Protocol
Results
Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium
Total cell conc. (cells/ml): 1.09 x 106
Live cell conc. (cells/ml): 1.07 x 106
Dead cell conc. (cells/ml): 1.78 x 104
Viability (%): 98.4
Average cell size (μm): 17.5
AsPC-1: Human pancreatic adenocarcinoma
Protocol
Results
Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: High
Total cell conc. (cells/ml): 7.51 x 105
Live cell conc. (cells/ml): 7.19 x 105
Dead cell conc. (cells/ml): 3.17 x 104
Viability (%): 95.8
Average cell size (μm): 10.7
C6： Rat glioma
Protocol
Results
Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium
Total cell conc. (cells/ml): 1.31 x 106
Live cell conc. (cells/ml): 1.22 x 106
Dead cell conc. (cells/ml): 8.91 x 104
Viability (%): 93.2
Average cell size (μm): 12.1
Capan-2： Human pancreatic adenocarcinoma
Protocol
Results
Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium
Total cell conc. (cells/ml): 1.04 x 106
Live cell conc. (cells/ml): 1.00 x 106
Dead cell conc. (cells/ml): 3.61 x 104
Viability (%): 96.5
Average cell size (μm): 13.4
DU 145： Human prostate carcinoma
Protocol
Results
Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium
Total cell conc. (cells/ml): 1.86 x 106
Live cell conc. (cells/ml): 1.80 x 106
Dead cell conc. (cells/ml): 5.34 x 104
Viability (%): 97.1
Average cell size (μm): 14.3
293-GFP： HEK293 stably expressing GFP
Protocol
Results
Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium
Total cell conc. (cells/ml): 1.84 x 106
Live cell conc. (cells/ml): 1.82 x 106
Dead cell conc. (cells/ml): 2.23 x 104
Viability (%): 98.8
Average cell size (μm): 11.3
H1299： Human non-small cell lung carcinoma
Protocol
Results
Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium
Total cell conc. (cells/ml): 4.14 x 105
Live cell conc. (cells/ml): 3.52 x 105
Dead cell conc. (cells/ml): 6.23 x 104
Viability (%): 84.9
Average cell size (μm): 19.5
H4： Human neuroglioma
Protocol
Results
Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium
Total cell conc. (cells/ml): 2.08 x 105
Live cell conc. (cells/ml): 1.44 x 105
Dead cell conc. (cells/ml): 6.32 x 104
Viability (%): 69.6%
Average cell size (μm): 12.8
HCT116： Human colorectal carcinoma
Protocol
Results
Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium
Total cell conc. (cells/ml): 1.34 x 106
Live cell conc. (cells/ml): 1.32 x 106
Dead cell conc. (cells/ml): 1.81 x 104
Viability (%): 98.7
Average cell size (μm): 12.2
HeLa： Human cervical carcinoma
Protocol
Results
Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium
Total cell conc. (cells/ml): 8.98 x 105
Live cell conc. (cells/ml): 8.11 x 105
Dead cell conc. (cells/ml): 8.69 x 104
Viability (%): 90.3
Average cell size (μm): 17.6
Hep3B： Human hepatocellular carcinoma
Protocol
Results
Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium
Total cell conc. (cells/ml): 1.54 x 106
Live cell conc. (cells/ml): 1.49 x 106
Dead cell conc. (cells/ml): 5.87 x 104
Viability (%): 96.2
Average cell size (μm): 14.9
HL-60： Human acute promyelocytic leukemia
Protocol
Results
Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium
Total cell conc. (cells/ml): 1.57 x 106
Live cell conc. (cells/ml): 1.45 x 106
Dead cell conc. (cells/ml): 1.23 x 105
Viability (%): 92.2
Average cell size (μm): 14.9
Hs 578T： Human mammary carcinoma
Protocol
Results
Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium
Total cell conc. (cells/ml): 1.13 x 106
Live cell conc. (cells/ml): 8.72 x 105
Dead cell conc. (cells/ml): 2.62 x 105
Viability (%): 76.9
Average cell size (μm): 16.5
MCF7： Human breast adenocarcinoma
Protocol
Results
Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium
Total cell conc. (cells/ml): 4.74 x 105
Live cell conc. (cells/ml): 4.43 x 105
Dead cell conc. (cells/ml): 4.07 x 104
Viability (%): 91.4
Average cell size (μm): 16.2
NIH3T3： Mouse embryonic fibroblast
Protocol
Results
Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium
Total cell conc. (cells/ml): 1.52 x 106
Live cell conc. (cells/ml): 1.33 x 106
Dead cell conc. (cells/ml): 1.96 x 105
Viability (%): 87.1
Average cell size (μm): 14.1
PANC-1： Human pancreatic ductal carcinoma
Protocol
Results
Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: High
Total cell conc. (cells/ml): 1.23 x 106
Live cell conc. (cells/ml): 1.21 x 105
Dead cell conc. (cells/ml): 2.26 x 104
Viability (%): 98.2
Average cell size (μm): 15.3
PC-3： Human prostate adenocarcinoma
Protocol
Results
Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium
Total cell conc. (cells/ml): 1.36 x 106
Live cell conc. (cells/ml): 1.33 x 106
Dead cell conc. (cells/ml): 3.16 x 104
Viability (%): 97.7
Average cell size (μm): 17.2
STO： Mouse embryonic fibroblast
Protocol
Results
Noise reduction: 5
Roundness: 0%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium
Total cell conc. (cells/ml): 1.78 x 106
Live cell conc. (cells/ml): 1.50 x 106
Dead cell conc. (cells/ml): 2.81 x 105
Viability (%): 84.2
Average cell size (μm): 13.1
U-2 OS： Human osteosarcoma
Protocol
Results
Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium
Total cell conc. (cells/ml): 7.66 x 105
Live cell conc. (cells/ml): 7.21 x 105
Dead cell conc. (cells/ml): 4.45 x 104
Viability (%): 94.2
Average cell size (μm): 16.0
U937： Human lymphoma
Protocol
Results
Noise reduction: 5
Roundness: 40%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium
Total cell conc. (cells/ml): 1.22 x 106
Live cell conc. (cells/ml): 1.12 x 106
Dead cell conc. (cells/ml): 1.02 x 105
Viability (%): 91.6
Average cell size (μm): 13.4
