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In this section, the featured culture of APM cells was immunofluorescently labeled with primary anti-clathrin (heavy chain) mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Texas Red in order to target the cytoskeletal network. In addition, peroxisomes present in the culture were simultaneously labeled with Oregon Green 488 conjugated to goat secondary antibodies directed against rabbit anti-PMP 70 (peroxisomal membrane protein 70) primary antibodies. Nuclei were counterstained with Hoechst 33258.
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