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オルガノイドとスフェロイドがin vivo条件を忠実に再現できる一方で、イメージングに基づく解析では、高い空間分解能により細胞固有の反応をモニタリングできます。 オリンパスでは患者由来のがんのオルガノイドとスフェロイドを使用して、イメージングに基づく三次元解析および医薬品評価方法を開発しています。

Three-dimensional cell culture models such as patient-derived organoids (PDO) and spheroids have increased in popularity because they can provide a 3D microenvironment that more closely reproduces in vivo conditions compared to 2D monolayer culture. Phenotypic and functional heterogeneity arise among cancer cells within the same tumor because of genetic change, environmental differences and reversible changes in cell properties. Therefore, evaluation of cell-specific responses is important for accurate prediction of drug efficacy and kinetics in vivo.