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3D Assays: Intelligent Software, Insightful Analyses

3D Assays: Intelligent Software, Insightful Analyses

In this webinar, we discuss how to obtain the best statistical data for spheroids and microplate-based assays. These data can help you quantify the response of a 3D model to a compound and makes it easier to compare the effect of different treatments, such as varying concentrations.

Presenters:

Daniel Bemmerl, Area Manager, High-End Microscopy Systems
Shohei Imamura, Strategic Project Manager, Regenerative Medicine and Drug Discovery Solutions

FAQ

Webinar FAQs | 3D Analysis

Can NoviSight™ software be used on images obtained with other microscope brands?

Yes, it can. While NoviSight 3D cell analysis software is tailored to Olympus image files, we provide a TIFF image converter for flexibility. As long as your images are in an open format, then you can import them into the software and run the analysis.

Can NovSight software analyze 2D images?

Yes, it can. This capability is useful for broader and more standard applications.

Can widefield microscopes do 3D imaging?

It depends on the sample thickness and required outcome of your experiment. If your sample is thicker than 2–30 μm (i.e., more than 2–3 cell layers) and you want to measure single-cell level information, 3D imaging with widefield microscopy is not recommended as it is hard to visualize the depth due to light scattering.

In contrast, if your sample is less than 2-30 μm or you just want a rough estimation of the size of tissue fragments or clustered cells, such as the volume of whole organoids, it might work depending on your sample specification. Still, we would recommend applying image processing like 3D deconvolution to enhance the contrast. Please keep in mind this is still less accurate than measurement on confocal microscopes, so it is only an intermediate solution.

Can 3D applications be used in fields other than drug discovery?

Yes, definitely. We have seen a strong increase in academic research moving toward 3D data applications. For example, organoids have been studied and used in the regenerative medicine and bioengineering fields.

What microscope is best for 3D live imaging?

A two-photon microscope (also called a multiphoton microscope) is the best fit to observe a 3D live-cell sample such as a spheroid or organoid. Generally, a two-photon microscope is gentler on cells and can observe at depth within a 3D spheroid.

Does NoviSight analysis software incorporate machine learning?

The current algorithms are tailor-made for organoids and spheroids but are also flexible enough to extend to tissues. Machine learning and deep learning might make sense in the future, but for now, voxel analysis works extremely well with the traditional algorithm approach.


関連製品

3次元細胞解析ソフトウェア

NoviSight

  • 3次元細胞解析により研究プロセスの効率化を実現
  • データを連動して見られるかつ自由自在なインターフェースで効率的な作業に貢献
  • 多様な観察手法と組み合わせた活用が可能

3D Assays: Intelligent Software, Insightful Analyses

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Webinar: 3D Assays: Intelligent Software, Insightful Analyses

In this webinar, our experts discuss how to obtain the best statistical data for spheroids and microplate-based assays. These data can help you quantify the response of a 3D model to a compound and makes it easier to compare the effect of different treatments, such as varying concentrations.

FAQ

Webinar FAQs | 3D Analysis

Can NoviSight™ software be used on images obtained with other microscope brands?

Yes, it can. While NoviSight 3D cell analysis software is tailored to Olympus image files, we provide a TIFF image converter for flexibility. As long as your images are in an open format, then you can import them into the software and run the analysis.

Can NovSight software analyze 2D images?

Yes, it can. This capability is useful for broader and more standard applications.

Can widefield microscopes do 3D imaging?

It depends on the sample thickness and required outcome of your experiment. If your sample is thicker than 2–30 μm (i.e., more than 2–3 cell layers) and you want to measure single-cell level information, 3D imaging with widefield microscopy is not recommended as it is hard to visualize the depth due to light scattering.

In contrast, if your sample is less than 2-30 μm or you just want a rough estimation of the size of tissue fragments or clustered cells, such as the volume of whole organoids, it might work depending on your sample specification. Still, we would recommend applying image processing like 3D deconvolution to enhance the contrast. Please keep in mind this is still less accurate than measurement on confocal microscopes, so it is only an intermediate solution.

Can 3D applications be used in fields other than drug discovery?

Yes, definitely. We have seen a strong increase in academic research moving toward 3D data applications. For example, organoids have been studied and used in the regenerative medicine and bioengineering fields.

What microscope is best for 3D live imaging?

A two-photon microscope (also called a multiphoton microscope) is the best fit to observe a 3D live-cell sample such as a spheroid or organoid. Generally, a two-photon microscope is gentler on cells and can observe at depth within a 3D spheroid.

Does NoviSight analysis software incorporate machine learning?

The current algorithms are tailor-made for organoids and spheroids but are also flexible enough to extend to tissues. Machine learning and deep learning might make sense in the future, but for now, voxel analysis works extremely well with the traditional algorithm approach.


関連製品

3次元細胞解析ソフトウェア

NoviSight

  • 3次元細胞解析により研究プロセスの効率化を実現
  • データを連動して見られるかつ自由自在なインターフェースで効率的な作業に貢献
  • 多様な観察手法と組み合わせた活用が可能
Experts
Daniel Bemmerl
Area Manager, High-End Microscopy Systems

Daniel Bemmerlと申します。3Dハイコンテント解析とオルガノイドのイメージングに関するあらゆる支援を提供しています。TIRF検鏡法とハイコンテントスクリーニングのアプリケーションスペシャリストとしてオリンパスに入社し、今は、3D解析ソフトウェアを専門としています。

私は分子および発生幹細胞生物学を学んでいましたが、そのうち、研究のより技術的な側面、特にイメージング対象の試料よりもイメージングそのものが面白いと感じるようになっていました。研究所以外の場所で、お客さまと開発とのインターフェイスとして働きたいと考え、オリンパスライフサイエンスに入社しました。3Dイメージングに関するあらゆる質問にお答えし、研究に集中してもらえるようにイメージングワークフローの向上のお手伝いができれば幸いです。

Shohei Imamura
プロジェクトマネージャー

今村尚平と申します。戦略製品のプロジェクトマネージャーをしています。ライフサイエンス向け顕微鏡のセールスで4年、ソフトウェアに的を絞った製品計画で7年の経験を積んでまいりました。また、戦略的プロジェクトのマネジメントと実行の仕事も行っています。日本の明治大学で商学士号を取得しています。

3D Assays: Intelligent Software, Insightful Analyses2021年10月26日
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