Embryonic Rat Thoracic Aorta Medial Layer Myoblast Cells (A-10 Line)
The technique of double immunofluorescence was employed to simultaneously label an adherent culture of rat thoracic aorta (A-10 line) cells with mouse anti-histone and rabbit anti-beta-catenin primary antibodies, followed by goat anti-mouse and anti-rabbit secondary antibodies conjugated to Marina Blue (histones) and Rhodamine Red-X (beta-catenin), respectively. The culture was counterstained with Alexa Fluor 488 conjugated to phalloidin, targeting the intracellular filamentous actin network.
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