Horse Dermal Fibroblast Cells (NBL-6)
In order to visualize the relationship between the Golgi complex and the intracellular microtubular network, a culture of horse dermal fibroblast (NBL-6) cells was immunofluorescently labeled with anti-tubulin (pan) mouse monoclonal primary antibodies followed by goat anti-mouse Fab fragments conjugated to Alexa Fluor 488. Golgi bodies were simultaneously targeted with rabbit anti-giantin primary antibodies, followed by goat anti-rabbit secondaries conjugated to Alexa Fluor 568. Nuclei were counterstained with Hoechst 33342.
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