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Human Bone Osteosarcoma Cells (U-2 OS Line)
The culture of human osteosarcoma cells showed in this section was immunofluorescently labeled with primary anti-vinculin mouse monoclonal antibodies followed by goat anti-mouse Fab heavy and light chain fragments conjugated to Cy3. Vinculin is a protein associated with focal adhesion and adheren-t junctions, which are membrane-associated complexes that serve as nucleation sites for actin filaments and as crosslinkers between the external medium, plasma membrane, and actin cytoskeleton. The cells were also stained with the DNA-specific fluorophore DAPI and an Alexa Fluor 488 phalloidin conjugate in order to target cytoskeletal F-actin.
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