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Embryonic Rat Thoracic Aorta Medial Layer Myoblast Cells (A-10 Line)

Embryonic Rat Thoracic Aorta Medial Layer Myoblast Cells (A-10 Line)

In what has become a standard fluorescence staining protocol, an adherent culture of rat thoracic aorta (A-10) cells was labeled with MitoTracker Red CMXRos before fixing, and the cells were subsequently stained with Alexa Fluor 488 conjugated to phalloidin, followed by Hoechst 33258, a popular DNA-binding counterstain.

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