Características

	The Olympus IX83 inverted microscope has been integrated into our IXplore systems. IXplore Systems are designed to provide solutions-based packages that suit your research application needs. Learn more

The Olympus IX83 inverted microscope has been integrated into our IXplore systems. IXplore Systems are designed to provide solutions-based packages that suit your research application needs.

Learn more

Expandable to Meet Growing Research Needs

The fully-motorized IX83 is designed to satisfy a variety of research needs. With additional modules providing expanded functionality, both microscope options enable a multitude of imaging techniques, ranging from casual documentation to long-term time-lapse imaging and other demanding techniques.
The unique open frame of the IX83 provides ready access to the light path, thus making it easy to add or change modules. A variety of deck modules can be easily exchanged to add or remove functions as needed. With a simple slide-in design, the TruFocus Z drift compensator module can be easily added to any IX83 system to maintain continuous focus throughout an extended time-lapse experiment.

IX83: Two-deck System

Enables high-speed, fully automated device selection during live cell research and advanced image acquisition. The two-deck configuration provides excellent expandability.

IX83: One-deck System

An intelligent, motorized microscope featuring a large field number (FN22, left side port) and TruFocus compatibility, thus creating a new standard for live cell imaging.

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Especificações

Método de observação > Fluorescência (Excitação azul/verde) ✓
Método de observação > Fluorescência (excitação ultravioleta) ✓
Método de observação > Contraste de interferência diferencial (DIC) ✓
Método de observação > Contraste de fase ✓
Método de observação > Campo claro ✓
Foco > Motorizado
✓
Foco > Compensador de desvio Z ✓
Tubos de observação > Campo amplo (FN 22) > Tubo binocular com inclinação variável ✓
Tubos de observação > Campo amplo (FN 22) > Trinocular ✓
Platina > Motorizado
Contact your local sales representative to hear about motorized stage options
Platina > Manual > Platina simples ✓
Platina > Mecânico > Platina mecânica IX3-SVR com pega à direita
X: 114 mm, Y: 75 mm
Condensador > Motorizado > Condensador universal NA 0.55/ W.D. 26.2mm
Condensador > Manual > Condensador universal Dry: NA 0.9/ W.D. 1.5 mm, Oil: NA 1.4/ W.D. 0.63 mm (1.25 X - 100 X)
Condensador > Manual > Condensador com distância de trabalho ultra longa NA 0,3/D.T. 73,3 mm
Dimensões (L × D × A) 323 (W) x 475 (D) x 686 (H) mm (1 Deck Standard Configuration)
Peso 47 kg (1 Configuração padrão de tabuleiro)

Método de observação > Fluorescência (Excitação azul/verde)		✓
Método de observação > Fluorescência (excitação ultravioleta)		✓
Método de observação > Contraste de interferência diferencial (DIC)		✓
Método de observação > Contraste de fase		✓
Método de observação > Campo claro		✓
Foco > Motorizado		✓
Foco > Compensador de desvio Z		✓
Tubos de observação > Campo amplo (FN 22) > Tubo binocular com inclinação variável		✓
Tubos de observação > Campo amplo (FN 22) > Trinocular		✓
Platina > Motorizado		Contact your local sales representative to hear about motorized stage options
Platina > Manual > Platina simples		✓
Platina > Mecânico > Platina mecânica IX3-SVR com pega à direita		X: 114 mm, Y: 75 mm
Condensador > Motorizado > Condensador universal		NA 0.55/ W.D. 26.2mm
Condensador > Manual > Condensador universal		Dry: NA 0.9/ W.D. 1.5 mm, Oil: NA 1.4/ W.D. 0.63 mm (1.25 X - 100 X)
Condensador > Manual > Condensador com distância de trabalho ultra longa		NA 0,3/D.T. 73,3 mm
Dimensões (L × D × A)		323 (W) x 475 (D) x 686 (H) mm (1 Deck Standard Configuration)
Peso		47 kg (1 Configuração padrão de tabuleiro)

Aplicação

Light-induced Oscillatory/Sustained Expression of Ascl1 in Neural Progenitor Cells.

For generating oscillatory expression of transgene, blue-light was illuminated for 1 min with a 3-hr interval, which maintaining proliferating NPCs.For achieving sustained expression of transgene, blue-light was illuminated for 2 min with a 30-min interval which promoting neuronal fate determination.
Image data courtesy of:
Itaru Imayoshi and Ryoichiro Kageyama
Institute for Virus Research, Kyoto University

Reference material:
Itaru Imayoshi, Akihiro Isomura, Yukiko Harima, Kyogo Kawaguchi, Hiroshi Kori, Hitoshi Miyachi, Takahiro Fujiwara, Fumiyoshi Ishidate, and Ryoichiro Kageyama "Oscillatory Control of Factors Determining Multipotency and Fate in Mouse Neural Progenitors" Science 1242366 Published online 31 October 2013.

Synthetic ERK Activity Propagation by A Light-Switchable System

Reference:
Kazuhiro Aoki, Yuka Kumagai,Atsuro Sakurai,Naoki Komatsu,Yoshihisa Fujita,Clara Shionyu,Michiyuki Matsuda. "Stochastic ERK Activation Induced by Noise and Cell-to-Cell Propagation Regulates Cell Density-Dependent Proliferation" Molecular Cell,17 October 2013.

Real-time Imaging for Ring1BYFP/YFP ME

Constitutive exchange of Ring1B-YFP at the PRC1 clusters. A PRC1 cluster in Ring1B YFP/YFP MEFs was subjected to photo-bleaching and subsequent sequential imaging.
Image data courtesy of:
Kazuhiro Aoki and Michiyuki Matsuda
Imaging Platform for Spatio-Temporal Information, Graduate School of Medicine, Kyoto University

Constitutive exchange of Ring1B-YFP at the PRC1 clusters. A PRC1 cluster in Ring1B YFP/YFP MEFs was subjected to photo-bleaching and subsequent sequential imaging.
Image data courtesy of:
Kyoichi Isono, and Haruhiko Koseki
Laboratory for Developmental Genetics, RIKEN Center for Integrative Medical Sciences (IMS-RCAI)

Reference:
SAM domain polymerization links subnuclear clustering of PRC1 to gene silencing. Isono K, et al. Developmental Cell, 26, 565-577 (2013).

Brain Slice Culture Glia Cell