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Fluorescence Microscopes

Fluorescence microscopy is used across a range of fields to gather high-quality images of small specimens, such as cells. In fluorescence microscopy, the sample is treated with fluorophores so that it re-emits light after it is excited by a light source, creating a high-resolution image. Our upright fluorescence microscopes use epifluorescence, also known as reflected light fluorescence, to maximize illumination of the sample. Browse upright epifluorescence microscopes in both semi- and fully motorized options with high-brightness LED light sources to easily acquire multicolor fluorescence images.

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Upright Fluorescence Microscopes

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Automated Fluorescence Microscope

BX63

  • Fully motorized system allows automation of complex multidimensional experiments
  • Accurate motorized Z-drive
  • High stability due to fixed stage design
Semi-Motorized Fluorescence Microscope

BX53

  • High-luminosity True Color LED illumination
  • Modular concept enables individual components to be motorized
  • Easily acquire multi-color fluorescence images
Biological Microscope

CX43

  • Ergonomic design
  • Ideal for versatile application
  • Long lifetime LED illumination

* Optional single line LED light source for fluorescence (peak excitation wavelength 470 nm only)

Upright Fluorescence Microscope FAQs

What is an upright fluorescence microscope?

An upright fluorescence microscope is a light microscope system that enables observation of the distribution of a single-molecule species based solely on the properties of fluorescence emission. Because it is an upright system, the sample is viewed from above. The sample must be prepared by treating it with fluorophores, which are chemical compounds that can re-emit absorbed light upon excitation. When you use an upright fluorescence microscope, the sample must also be sandwiched between a glass slide and a coverslip. Once excited by the light source, the sample re-emits light of a different wavelength that is then captured by the spectral emission filter to generate the image.

What is the difference between an upright and inverted fluorescence microscope?

The light path of a fluorescence microscope depends on a variety of factors, including the type of microscope. With an upright fluorescence microscope, the objectives are positioned above the specimen, whereas with an inverted fluorescence microscope, the objectives are beneath the stage and specimen. With an inverted microscope, the specimen can remain in its culture vessel or petri dish when the user observes it. With upright systems, samples are basically observed on a glass slide with a cover slip. This prevents the sample and objective from colliding. With both inverted and upright fluorescence microscopes, the specimen is treated with fluorophores.

When would you use an upright fluorescence microscope?

Upright fluorescence microscopes are the cost-effective workhorses of fluorescence imaging. For labs performing high-volume fluorescence observation, such as in life science research, upright epifluorescence microscope systems from Evident provide consistently high-quality bright, multicolor fluorescence images. Inverted epifluorescence microscopes are recommended when advanced imaging involving live-cell observation is required.

Fluorescence Microscopy Resource Videos

cellSens™ Imaging Software

This video shows you how to use cellSens imaging software with the DP74 digital microscope camera to improve your images acquired with upright fluorescence microscopes.

X Line™ High-Performance Objectives

Equip your Olympus upright fluorescence microscopes with X Line objectives to acquire accurate, bright multicolor fluorescence images with high image flatness and low chromatic aberration. See our novel polishing technique that enables us to manufacture these optical barrier-breaking objectives.

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