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应用资料

Support Biopsy Efficiency with the Simplified Operation of the IX73 Inverted Microscope


The Challenge

With objectives of lowering the miscarriage rate and improving the implantation rate, preimplantation genetic testing (PGT) is rising in popularity worldwide; therefore, the number of embryo biopsies performed is increasing accordingly.

In an embryo biopsy, some cells are removed from the embryo under an inverted microscope using relief contrast observation. The appropriate contrast adjustment is required to determine the location to irradiate the laser and the suction the cells, but complex microscope operations can cause user fatigue.

Olympus’ Solution to Improve the Efficiency of the Biopsy Workflow

Olympus’ relief contrast enables observation of 3-dimensional oocytes in plastic dishes. Since the relief contrast imaging is unaffected by the polarizing effect of the plastic dish, you can clearly view the oocyte zona pellucida to check its condition.

In addition, the dedicated remote hand switch of the IX73 semi-motorized system makes it easy to achieve suitable contrast and magnification/condenser settings with the push of a button. Streamlining the microscope operation to a minimum enables users to concentrate on the delicate biopsy manipulations, which require careful attention. Since the motorized operation using the hand switch reduces user intervention above the stage, it also decreases the risk of accidentally touching the sample or needle of the injector.

ハンドスイッチ

Remote hand switch

画像提供元: Cooper Surgical, Inc.

Samples and images data are courtesy of Cooper Surgical Inc.

Summary of the IX73 Semi-Motorized System’s Advantages for Embryo Biopsies

The IX73 system’s remote hand switch simplifies and streamlines microscope operations, supporting reliable and efficient embryo biopsies. The improved microscope operation workflow helps you manage the increased embryo biopsy workload.
 

Comment by Dr. Watanabe

When performing an embryo biopsy using the IX73 semi-motorized system, I am impressed by the smooth control of the magnification and laser system with the dedicated hand switch. In the past, when changing the magnification, we had to touch the condenser and objective lens, which required careful operation. I, like many users, have experienced shaking the microscope by accidentally touching it too forcefully. The IX73 semi-motorized system enables you to perform these operations by lightly pressing a switch without touching the microscope, so you can focus more on the embryo biopsy itself.

This application note was prepared with the help of the following researcher:
Dr. Hideaki Watanabe
Sugiyama Clinic Shinjuku
Center for Reproductive Medicine and Implantation Research

IX73 SL-ICSI

IIX3-ICSI/IMSI inverted microscope

适于这类应用的产品

ICSI / IMSI平台

IX3-ICSI/IMSI

IX3-ICSI系统有利于作为体外受精(IFV)过程组成部分的显微受精(ICSI)的使用。奥林巴斯的浮雕相衬法可在塑料培养皿中进行3D卵母细胞观察,以检查透明带状况。IX3半电动系统具有ICSI专用手动开关,只需简单地按一下按钮,即可立即更改观察方法和放大倍率。采用偏光法观察主轴及其位置,以确认卵母细胞成熟度和避免注射过程中的损伤,并采用微分干涉对比(DIC)法进行卵胞浆内形态选择性精子注射(IMSI)。

  • 为提高ICSI工作流程顺畅度进行了优化
  • 通过主轴可视化轻松检查卵母细胞状况
  • 通过电动操作简化了ICSI步骤

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