Life Science Solutions

Darkfield Interactive Tutorials

These Java-based interactive tutorials are designed to complement text pages by allowing students to use a web browser to simulate configuration and operation of a microscope under darkfield illumination. Both the theory and practice of darkfield microscopy are addressed by the tutorials.

  • Darkfield Abbe Condensers

    Explore how changes in the size of the opaque stop in a simple Abbe darkfield illumination condenser affects light cone shape and numerical aperture in this interactive tutorial.

  • Darkfield Condenser Adjustment

    Visitors and students are allowed to experiment with the adjustable parameters in condenser alignment and configuration for darkfield microscopy in this interactive java tutorial.

  • Specimen Illumination With Darkfield Condensers

    Discover how a darkfield microscopy specimen scatters light into the objective when illuminated with a hollow cone of oblique illumination from a Cardioid darkfield condenser.

  • Catadioptric Darkfield Reflected Light Objectives

    Discover how light that is diffracted, refracted, and reflected by a specimen enters the front lens of a darkfield objective in reflected light microscopy.

Contributing Authors

Mortimer Abramowitz - Olympus America, Inc., Two Corporate Center Drive., Melville, New York, 11747.

Kirill I. Tchourioukanov and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.

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