The data tables below contain reference information on fluorescence cubes offered by Olympus America, Inc. to support their microscopes. The codes for the excitation columns in Tables 2 are listed in Table 1. Table 2 is a listing of examples of cubes’ configuration of filters and mirrors. The authors wish to caution that we believe this data to be accurate, but it should always be verified by authorized Olympus representatives.
Code | Filter Type |
---|---|
U | Ultraviolet |
V | Violet |
BV | Blue-Violet |
B | Blue |
IB | InterferenceBlue Filter |
G | Green |
IG | Immuno GoldStaining Method |
BF | Brightfield,Phase Contrast,and DIC only |
Table 2 lists the excitation and barrier filters by cube along with the dichroic mirror used in each cube. Use this chart as an aid to guide you in selection of some of the Olympus U-URA cubes for applications listed in our fluorescence and fluorochrome data tables.
Discover how variations in excitation and barrier filters allow certain wavelengths to pass.
Excitation | Type | Excitation Filter(nm) | Dichroic Mirror(nm) | Barrier Filter |
---|---|---|---|---|
U | Wide | 330-385 | 400 | 420-- |
U | Narrow | 360-370 | 400 | 420-- |
U | Narrow Bandpass | 360-370 | 400 | 420-460 |
V | Narrow | 400-410 | 455 | 455-- |
BV | Wide | 400-440 | 455 | 475-- |
BV | Narrow | 420-440 | 455 | 475-- |
B | Wide | 450-480 | 500 | 515-- |
IB | Wide, Interference | 460-490 | 505 | 515IF-- |
IB | Narrow, Interference | 470-490 | 505 | 515IF-- |
G | Super Wide | 480-550 | 570 | 590-- |
G | Wide | 510-550 | 570 | 590-- |
G | Narrow | 530-550 | 570 | 590-- |
IG | Narrow, Interference | 520-550 | 565 | 580IF-- |
IY | Wide, Interference | 545-580 | 600 | 610IF-- |
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