Keep Your Samples Moving

Automated Motorized Stage Tracking

Keeping moving samples, like C. elegans, in a microscope’s field of view during time-lapse imaging is challenging. Our automated motorized stage tracking software for FLUOVIEW laser scanning microscopes helps solve this challenge. Easy to operate, the software detects the target object in relation to the XY stage’s centroid position during time-lapse image acquisition and moves the stage to keep the target object near the center of the field of view.

With two detection algorithms for fluorescence and transmission images, the software is designed for XYT/XYZT* time-lapse imaging.

*Only the horizontal direction (XY coordinates) is automatically adjusted in this software

Image data are courtesy of the following institution:
Asuka Takeishi Ph.D.(RIKEN Hakubi Team Leader)
Neural Circuit of Multisensory Integration, RIKEN Hakubi Research Team.

Sample condition: C. elegans labeled green (muscle cells, myo-3p::GCaMP3) and red (coelomocyte, unc-119p::dsRed)
Acquisition condition: UPLXAPO4X with 1.8x zoom, resonant one-way, 512 x 512, 15 fps
Tracking detection condition: Automatic detection based on dsRed (red) fluorescence intensity/size.

"We were in dire need of tracking software that could acquire multicolor fluorescent images of neurons while automatically tracking fast-moving C. elegans.
This software allows us to track C. elegans consistently for long periods of time while acquiring images with high-resolution confocal microscopy. 
The software powers our research by allowing us to easily observe details of nematode behavior and neural activity simultaneously."

Asuka Takeishi, Ph.D.
RIKEN Hakubi Team Leader,
Neural Circuit of Multisensory Integration, RIKEN Hakubi Research Team.