FLUOVIEW Technologies

Advance Your Research with 4 Innovative Technologies for FLUOVIEW

TruSpectral: High light efficiency for bright, precise multicolor images.
TruSight: Seamless deconvolution for clearer, sharper images.
TruFocus: Stable time-lapse images with low phototoxicity.
TruResolution: Automated correction for brighter and sharper images at depth.

_{Brainbow AAV transfection of Purkinje cells amplified with antibodies as described in Cai et al 2013. Visible are Purkinje cell somata, dendrites and axons, as well as some aspecific stainings of granule cells.}	TruSpectral - High Sensitivity Multi-Color Imaging (Compatible with FV3000) An integrated feature of all FLUOVIEW FV3000 confocal systems, TruSpectral technology enables much higher light throughput compared to conventional spectral detection units. TruSpectral technology employs volume phase holograms that diffract light more efficiently than traditional reflection gratings, delivering up to three-fold higher transmission for red and far-red wavelengths. The result is excellent multicolor fluorescence with low excitation light for live and fixed tissue imaging. Read a TruSpectral app note Learn more about TruSpectral

TruSpectral - High Sensitivity Multi-Color Imaging

_{Brainbow AAV transfection of Purkinje cells amplified with antibodies as described in Cai et al 2013. Visible are Purkinje cell somata, dendrites and axons, as well as some aspecific stainings of granule cells.}

TruSpectral - High Sensitivity Multi-Color Imaging

(Compatible with FV3000)

An integrated feature of all FLUOVIEW FV3000 confocal systems, TruSpectral technology enables much higher light throughput compared to conventional spectral detection units. TruSpectral technology employs volume phase holograms that diffract light more efficiently than traditional reflection gratings, delivering up to three-fold higher transmission for red and far-red wavelengths. The result is excellent multicolor fluorescence with low excitation light for live and fixed tissue imaging.

Read a TruSpectral app note

Learn more about TruSpectral

TruSight - GPU Based Deconvolution

(Compatible with FV3000, FVMPE-RS)

Remove blur and obtain clearer, sharper images with TruSight deconvolution. Specialized cellSens algorithms for the FLUOVIEW FV3000 confocal microscope and FVMPE-RS multiphoton microscope enable a seamless workflow from acquisition to publication with the click of a button.

Learn more about TruSight

Left: Raw confocal image / Right: Image with TruSight^*

_{HeLa cells

Blue: nuclei (DAPI), Green: microtubules (Alexa Fluor 488), Red: mitochondria (MitoTracker Red)}

Left: Raw confocal image / Right: Image with TruSight^*

_{Nucleopore

Nup153 (Alexa Fluor 488), Nup62 (Alexa Fluor 555)

Image data courtesy of: Dr. Hidetaka Kosako, Fujii Memorial Institute of Medical Sciences, Tokushima University.}

Related Videos _{3D imaging NK cells attacking spheroid HT-29 tumor cells for 48 hours. Image data courtesy of: Dr. Yuji Mishima, The Cancer Chemotherapy Center of JFCR.}	TruFocus - Stable Time-Lapse Imaging (Compatible with FV3000, FVMPE-RS) The TruFocus system makes multiposition experiments and long-term, time-lapse imaging more robust and reliable. The system uses a minimally phototoxic beam to accurately maintain the focus position, so you don’t have to worry about thermal drift caused by a change in room temperature or drug delivery during experiments. Read a TruFocus app note Learn more about TruFocus

_{3D imaging NK cells attacking spheroid HT-29 tumor cells for 48 hours.

Image data courtesy of: Dr. Yuji Mishima, The Cancer Chemotherapy Center of JFCR.}

TruFocus - Stable Time-Lapse Imaging

(Compatible with FV3000, FVMPE-RS)

The TruFocus system makes multiposition experiments and long-term, time-lapse imaging more robust and reliable. The system uses a minimally phototoxic beam to accurately maintain the focus position, so you don’t have to worry about thermal drift caused by a change in room temperature or drug delivery during experiments.

Read a TruFocus app note

Learn more about TruFocus

TruResolution - Maximize Resolution in Deep Imaging (Compatible with FVMPE-RS) Maximize the deep imaging performance of your multiphoton microscope with TruResolution objectives. An automated correction collar compensates for spherical aberration at every depth, delivering bright, high-resolution images from the top to bottom of your image stack. Read the white paper Learn more about TruResolution	_{Maximum projection of mouse in vivo brain images acquired at around 600 um depth (Thy1-YFP-H mouse, sensory cortex). Image data courtesy of: Dr. Hiromu Monai, Dr.Hajime Hirase and Dr. Atsushi Miyawaki, RIKEN BSI-Olympus Collaboration Center.}

TruResolution - Maximize Resolution in Deep Imaging

(Compatible with FVMPE-RS)

Maximize the deep imaging performance of your multiphoton microscope with TruResolution objectives. An automated correction collar compensates for spherical aberration at every depth, delivering bright, high-resolution images from the top to bottom of your image stack.

Read the white paper

Learn more about TruResolution

TruResolution - Maximize Resolution in Deep Imaging

_{Maximum projection of mouse in vivo brain images acquired at around 600 um depth (Thy1-YFP-H mouse, sensory cortex).

Image data courtesy of: Dr. Hiromu Monai, Dr.Hajime Hirase and Dr. Atsushi Miyawaki, RIKEN BSI-Olympus Collaboration Center.}

Related Products

FV3000 Confocal Laser Scanning Microscope

Available with either galvanometer-only (FV3000) or hybrid galvanometer/resonant (FV3000RS) scanner configurations
Highly efficient and accurate TruSpectral detection on all channels
Optimized for live cell imaging with high sensitivity and low phototoxicity

Learn more

FVMPE-RS Multiphoton Laser Scanning Microscope

Maximize the resolution and contrast with TruResolution objectives
High-speed imaging with a resonant scanner
Extended infrared multiphoton excitation up to 1300 nm
Triple scanner option for multiphoton and visible light laser stimulation
Highly efficient transmission with Olympus 1600 optical coatings on multiphoton dedicated objectives and scanning unit
4-axis auto-alignment of the infrared laser beams

Learn more

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Advance Your Research with 4 Innovative Technologies for FLUOVIEW

TruSpectral - High Sensitivity Multi-Color Imaging

(Compatible with FV3000)

TruSight - GPU Based Deconvolution

(Compatible with FV3000, FVMPE-RS)

Related Videos

TruFocus - Stable Time-Lapse Imaging

(Compatible with FV3000, FVMPE-RS)

TruResolution - Maximize Resolution in Deep Imaging

(Compatible with FVMPE-RS)

Related Products

FV3000 Confocal Laser Scanning Microscope

FVMPE-RS Multiphoton Laser Scanning Microscope

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