TruSpectral Technology

Bright Multicolor Fluorescence Integrated in FV3000 confocal systems, TruSpectral technology enables much higher light throughput compared to conventional spectral detection units. Volume phase holograms diffract light more efficiently than traditional reflection gratings, delivering up to three-fold higher transmission for red, far-red and near infra-red wavelength Bright multicolor fluorescence with low excitation light for live and fixed tissue imaging
_{Mouse mPFC labeled with glial fibrillary acidic protein (GFAP; astrocyte marker; yellow), calmodulin-dependent protein kinase II (CaMKII; pyramidal neuron marker; red), amphoterin-induced protein 1 precursor (AMIGO-1; neuronal membrane marker; cyan), parvalbumin (PV; inhibitory neuron marker; purple), ankyrin-G (AnkG; axon initial segment marker; green), and nuclear yellow (nuclei marker; blue).} _{Image data courtesy of Stephanie Shiers, Ph.D. Candidate, Theodore J. Price, Ph.D., Price Lab, Center for Advanced Pain Studies, Department of Neurobiology, School of Behavioral and Brain Sciences, University of Texas at Dallas.}

Bright Multicolor Fluorescence

Integrated in FV3000 confocal systems, TruSpectral technology enables much higher light throughput compared to conventional spectral detection units.

Volume phase holograms diffract light more efficiently than traditional reflection gratings, delivering up to three-fold higher transmission for red, far-red and near infra-red wavelength
Bright multicolor fluorescence with low excitation light for live and fixed tissue imaging

_{Mouse mPFC labeled with glial fibrillary acidic protein (GFAP; astrocyte marker; yellow), calmodulin-dependent protein kinase II (CaMKII; pyramidal neuron marker; red), amphoterin-induced protein 1 precursor (AMIGO-1; neuronal membrane marker; cyan), parvalbumin (PV; inhibitory neuron marker; purple), ankyrin-G (AnkG; axon initial segment marker; green), and nuclear yellow (nuclei marker; blue).}

_{Image data courtesy of Stephanie Shiers, Ph.D. Candidate, Theodore J. Price, Ph.D., Price Lab, Center for Advanced Pain Studies, Department of Neurobiology, School of Behavioral and Brain Sciences, University of Texas at Dallas.}

TruSpectral - High Sensitivity Multi-Color Imaging

Related Videos	Sensitivity and Accuracy TruSpectral detectors combine high sensitivity with spectral flexibility to detect even the dimmest fluorophores. Independently adjustable channels to optimize signal detection for each fluorophore Lambda scanning mode enables accurate spectral unmixing of complex overlapping fluorescent signals Variable barrier filter mode provides simultaneous four-channel image acquisition, with up to sixteen channels in virtual channel mode Adjustable slit to select the detection wavelengths of each individual channel down to 2 nm Optional Cooled Gallium Arsenide Phosphide (GaAsP) PMT detector to maximize detection efficiency

Sensitivity and Accuracy

TruSpectral detectors combine high sensitivity with spectral flexibility to detect even the dimmest fluorophores.

Independently adjustable channels to optimize signal detection for each fluorophore
Lambda scanning mode enables accurate spectral unmixing of complex overlapping fluorescent signals
Variable barrier filter mode provides simultaneous four-channel image acquisition, with up to sixteen channels in virtual channel mode
Adjustable slit to select the detection wavelengths of each individual channel down to 2 nm
Optional Cooled Gallium Arsenide Phosphide (GaAsP) PMT detector to maximize detection efficiency

Learn More

Development of a New Fucci(CA) Application: A Fluorescent Probe for Visualizing Cell Cycles

Read the app note

Related Products

FV3000

Available in galvanometer-only (FV3000) or hybrid galvanometer/resonant (FV3000RS) scanner configurations
Highly efficient and accurate TruSpectral detection on all channels
Optimized for live cell imaging with high sensitivity and low phototoxicity

Learn More

Innovative Tru technologies help researchers overcome the challenges of imaging experiments.

Seamless deconvolution for clearer, sharper images.

Stable focus throughout time-lapse experiments.

Automated correction for brighter and sharper images at depth.

Accurate and efficient image analysis based on deep-learning technology.

If you already have your personal ID enter it here:

If you already have your personal ID enter it here:

Bright Multicolor Fluorescence

Related Videos

Sensitivity and Accuracy

Learn More

Multiplexing with the FLUOVIEW FV3000 Confocal Microscope

3D Time-Lapse Imaging of Spheroids with the FLUOVIEW FV3000 Confocal Microscope

3D Observation of Cleared Mouse Liver Using the FLUOVIEW FV3000 Microscope

Development of a New Fucci(CA) Application: A Fluorescent Probe for Visualizing Cell Cycles

Related Products

FV3000

Innovative Tru technologies help researchers overcome the challenges of imaging experiments.

Contact Us

Redirecting