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Alexa Fluor 568 conjugated to phalloidin was utilized to label a 3T3 cell culture for intracellular filamentous actin, while DAPI was used to target DNA in the cell nuclei. The cells were simultaneously stained with Alexa Fluor 488 conjugated to concanavalin A, a lectin displaying very high affinity for specific carbohydrate residues in glycoproteins, enzymes, and cell membranes.
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