Life Science Solutions
Madin-Darby Ovine Kidney Epithelial Cells (MDOK Line)
In order to label the intermediate filaments in a log phase adherent MDOK culture, the fixed and permeabilized cells were blocked and treated with mouse anti-vimentin (porcine eye lens) primary antibodies followed by goat anti-mouse secondary antibodies (IgG) conjugated to Texas Red-X. Filamentous actin was visualized with phalloidin conjugated to Oregon Green 488, while the nuclei were stained with Hoechst 33258.
Sorry, this page is not
available in your country.