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The RMC cell line was derived from the kidney tissue of a 3-month-old male rat (Rattus norvegicus) belonging to the Sprague-Dawley strain. The mesangial cells were immortalized at passage eight with the plasmid pSV3-Neo, which codes simian virus 40 (SV40) large T-antigen, and are maintained in the presence of the antibiotic, G-418.
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