Evident LogoOlympus Logo

NUMERICAL APERTURE


An objective’s numerical aperture (NA) is a measure of its ability to gather light. This is an important factor in determining the resolution, depth of focus, and the brightness of images. Objectives with a larger NA gather a wider range of light, resulting in brighter, higher resolution images. NA is important when observing extremely fine structures or detecting dim signals during fluorescence observation.

Technical Background

Thanks to our novel manufacturing technology, X Line objectives, such as the UPLXAPO40XO, are able to maintain an NA of 1.40 without deteriorating other aspects of optical performance.

Improved NA with no deterioration in the chromatic correction range

Higher NA delivers higher resolution and signal-to-noise ratio

To design objectives with high numerical aperture (NA), the effective diameter of the lens needs to be increased. In order to improve the NA while maintaining the total length (45 mm) of the objective lens, it is necessary to make the edge of the convex lens extremely thin.

Conventional convex

X Line ultra-thin convex lens.
Larger angles lead to higher NA of the objective

Higher NA thanks to X Line’s objective's ultra-thin lens design

X Line objective’s high NA enables efficient experiments

The UPLXAPO40XO X Line objective has an NA of 1.40, which is equivalent to conventional 60X or 100X objectives, while having a much bigger field of view (FOV). This enables the user to acquire images with adequate resolution over a large area using just the 40X objective, as shown below. There is no need to change objectives or add oil, and the risk of losing the sample’s position is minimized.

Conventional convex

X Line ultra-thin convex lens.
Larger angles lead to higher NA of the objective

UPLXAPO40XO X Line objective (NA 1.40)

Your benefits from high numerical aperture for research imaging:

  • Gather more light for brighter imaging

  • Create high-resolution images

  • Minimize phototoxicity and photobleaching for live cell imaging

Your benefits from high numerical aperture for clinical imaging:

  • Gather more light for brighter imaging

  • Create high resolution images

  • Optimum sharpness and contrast

Chromatic
Correction

Image
Flatness

Read
Whitepaper


X Line Objectives Offer Revolutionary Optical Performance Thanks to Advanced Manufacturing Technology

Download PDF

Imaging applications


Previous
Live Cell Imaging

Live Cell Imaging

Learn more

Super Resolution and TIRF Imaging

Super Resolution and TIRF Imaging

Learn more

Neuroscience and Multiphoton Imaging

Neuroscience and Multiphoton Imaging

Learn more

Pathology and Laboratory

Pathology and Laboratory

Learn more

Drug Discovery

Drug Discovery

Learn more

Whole Slide Imaging

Whole Slide Imaging

Learn more

Laser Scanning Confocal

Laser Scanning Confocal

Learn more

Cancer Research and Multiplexing

Cancer Research and Multiplexing

Learn more

Fluorescence Imaging

Fluorescence Imaging

Learn more

Organoid and 3D Imaging

Organoid and 3D Imaging

Learn more

Next
 

See All Applications


Sorry, this page is not
available in your country.

Sorry, this page is not
available in your country.

Lo sentimos, la página solicitada no se encuentra disponible en su país.