Multiphoton Laser Scanning Microscope
The Olympus FVMPE-RS multiphoton imaging system is purpose-built for deep imaging in biological tissue, aimed at revealing both detail and dynamics. Innovative features for efficient delivery and detection of photons in scattering media enable high signal-to-noise ratio acquisition. This translates to bright images with precise details — even from deep within the specimen. High sensitivity is matched with high-speed imaging to capture rapid in vivo responses. For advanced applications,
dual-wavelength excitation extending to 1300 nm is available. Independent control of visible or multiphoton laser light stimulation and the ability to synchronize with patch clamp data are also possible.
Multiple configurations are available on the FVMPE-RS platform; each imaging system is customizable to meet your unique research requirements.
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Olympus TruResolution objectives maximize resolution and contrast for 3D imaging deep within thick specimens. The objectives are equipped with a motorized correction collar that can automatically and dynamically compensate for spherical aberration while maintaining focus position.
Learn more about the TruResolution Objectives
Deep focus mode adjusts the diameter of the laser beam based on the laser scattering conditions. For in vivo specimens with heavy laser scattering, the beam is narrowed so that more excitation photons reach deeper within your sample, helping produce bright, high-resolution images.
A laser beam with optimally adjusted pulse widths can be delivered to the focal plane, thanks to the application of negative dispersion that perfectly corresponds to the magnitude of the pulse-width dispersion generated during transmission through the microscope optics. The result is brighter images without needing to increase laser power, sample heating, photobleaching and phototoxicity.
Olympus dedicated multiphoton objectives are optimized for deep imaging under in vivo and cleared tissue conditions. A diverse lineup provides you the opportunity to select an objective according to your research requirements. Different optical designs emphasize high numerical aperture, long working distance, wide field of view, and compatibility with a range of immersion media and tissue clearing agents.
Learn more about the Multiphoton Objectives
The UPLSAPO30XSIR objective applies the efficient IR transmission coating of MPE dedicated objectives to a high numerical aperture silicone oil immersion objective. This combination makes the objective well-suited to multiphoton imaging of live cell specimens. The closer refractive index matching between silicone oil and live cells minimizes image distortion in the Z-direction and improves image brightness and resolution.
During time-lapse imaging, the refractive index of silicone oil remains constant, and the oil does not dry out, minimizing the amount of time researchers need to spend tending the experiment.
Olympus multiphoton objectives and scanner optics have an optical coating that offers excellent transmission from 400 nm to 1600 nm. Efficient infrared transmission translates to more available power for fluorescence excitation at depth while strong support at short wavelengths maintains efficient collection of fluorescence and harmonic emissions.
A fast resonant scanner and conventional galvanometer scanner provide high-speed and high-resolution imaging in a single system. Avoid motion artifacts when imaging dynamic samples with capture rates of 30 fps at 512 × 512 pixels at the full field of view (FN 18) or up to 438 fps at 512 × 32 pixels. These speeds enable applications such as tracking of fast moving cells in blood flow, and observing rapid membrane potential dynamics across neurons and other cells.
Olympus silver-coated scanner mirrors help deliver more laser power to your sample to better excite fluorescence, and yield brighter images. The silver-coated mirrors achieve very high re-flectance across a broad wavelength range, from visible to near infrared. The total reflectance for the XY scanner is particularly improved in the near infrared range compared to conventional aluminum-coated mirrors. The increased reflectance helps maximize available laser power and deliver the power needed for deep in vivo experiments.
High signal-to-noise ratio imaging can be acquired even from faint fluorescence through the use of high sensitivity gallium arsenide phosphide (GaAsP) photomultiplier tube (PMT) detectors. — GaAsP PMTs deliver greater quantum efficiency than standard multialkali PMTs. Fan-less Peltier cooling further improves the signal-to-noise ratio. You can also leverage the advantages of both detector types by combining them in a single system.
Greater Fluorescence Capture
The non-descanned detection path has been designed for greater light efficiency—it is positioned close to the specimen and features large area optics to better capture scattered fluorescence photons.
A hardware sequencer provides microsecond precision timing for stimulation and triggering events. Stimulation can be spatially and temporally synchronized to the imaging scan, facilitating the capture of fast response dynamics at precise locations. In the context of electrophysiology and optogenetics, this could mean the difference between distinguishing a synchronous versus an asynchronous stimulus response. For acquisitions lasting two weeks or longer or experiments with complex procedures that require switching between imaging tasks, the sequence manager software module maintains millisecond precision, providing high-quality data in demanding in vivo and in vitro experiments.
Learn more about multi-dimensional and multi-area time-lapse imaging
The FVMPE-RS imaging platform supports a dual wavelength infrared pulsed laser or two independent infrared lasers for multichannel, multiphoton excitation imaging. You can optimally excite different fluorophores without having to repeatedly tune the laser. Simultaneous excitation with independent power control of each laser line enables users to capture balanced images of different fluorophores. Separate excitation wavelengths for individual fluorophores can also reduce background tissue autofluorescence by shifting excitation away from the 800 nm range.
Quadralign 4 axis laser alignment simplifies system upkeep by maintaining the precise alignment of the excitation beam into the scanner unit, even in the face of laser drift due to wavelength tuning, temperature fluctuation, and other sources of cavity shift. The beam position and angle are automatically adjusted to deliver higher laser power and consistent pixel registration. If your system has two excitation laser lines, this feature offers an additional benefit. Auto laser alignment maintains co-alignment between the beams, helping eliminate co-registration errors between channels. The laser alignment can also be manually fine-tuned using the software interface.
The SIM scanner, an independent galvanometer scanner, and visible laser modules can be added for precise microsecond photostimulation and photobleaching experiments. On systems with two IR imaging lines, the SIM scanner enables simultaneous multiphoton stimulation and imaging.
Learn more about the SIM Scanner
Analog inputs and TTL I/O are available to support electrophysiology experiments. The analog input unit records external voltage signals as images that are treated the same way as normal image data. Light-stimulated electrical signals measured with patch clamps can be synchronized with image capture and displayed as a pseudocolor intensity overlay.
Learn more about the Analog Unit
Raster scanning takes time. With MMASW(Multi-Point Mapping Software) multi-point acquisitions you can eliminate that time by placing the laser only where needed, retaining high signal to noise output and allowing you the freedom to choose and optimize your scan path. A scan of multiple positions can go as fast as 101 Hz, and each position can gather signals at up to 50,000 Hz, providing you with highly relevant physiological data.
Multi-point mapping software is designed for extremely fast functional measurements in living cells or tissues where researchers use light to probe fluorescent intensity changes. Resonance scanners or acousto-optic deflectors (AODs) are intrinsically less sensitive due to their very short integration times and the number of photons that can be detected. By retaining high integration time per position where it is needed in a single point laser scan, the multi-point mode allows greater multiphoton depth penetration and signal-to-noise. Each point can also be expanded to an array for larger area stimulation or detection.
Synchronize your measurement scans with simultaneous stimulation using the SIM scanner and free your functional imaging.
Learn more about the Multi-Point Mapping Software
To achieve highly targeted laser light stimulation, the observation field is divided into a grid, and the laser illuminates each area in a pseudorandom sequence that avoids sequential stimulation of adjacent areas. A stimulation reaction map is drawn based on patch clamp recording or imaging intensity. Integration of an optional piezo nosepiece extends the reaction map to 3D, with stimulation delivered at depths different from the imaging plane.
Customize which controls you see in the software interface and where they are located. Save and reload your favorite layouts.
Save the acquisition parameters that you use during an experiment. The parameters can be easily recalled for repeatable imaging conditions.
The sequence manager makes it easy to coordinate experiments. Complex protocols, such as changing the frame rate during time-lapse imaging or repeating photostimulation events at different positions during image acquisition, can be organized and accurately carried out with precise timing. Protocols can also be saved and later reloaded for consistent execution of experiments.
See your entire specimen at high resolution and in context with the tiling function. This software feature scans multiple adjacent images and stitches them together. With a motorized stage, images can be stitched together in a very wide field of view, while the mapping feature makes it easy to locate the position of specific cells in the larger image.
The FVMPE-RS imaging platform’s software is integrated with Olympus cellSens image analysis software, expanding the system’s analytical capabilities. Optional features include 3D deconvolution for Z-stack images, area estimation for each particle in an image, an image processing filter, and colocalization analysis.
Closely overlapping fluorescence spectra can complicate biological studies that look at multiple labels simultaneously. Separation of overlapping spectral channels is possible via spectral deconvolution based on either a blind unmixing algorithm or previously saved multichannel profiles. Cross-talk between the channels can even be eliminated during image acquisition via live processing.
Large amounts of Z-stack data can be rendered into a 3D display. Important views can be registered as key frames, making it easy to create animated views of 3D images that zoom and transition to different camera angles.
Upright Microscope System — Designed for Multiphoton Microscopy
This Upright Frame is completely dedicated to multiphoton microscopy. Providing space for large samples, a high degree of motorization and nosepiece focus control enables the stage and your sample to remain fixed and stable.
Learn more about the Upright Microscope System
Gantry Microscope System — For in vivo Observation that Require Maximum Space
With its ultra-stable arch-like structure, the new Gantry Frame offers tremendous space beneath the objective lens along with a high degree of flexibility to suit different samples. This is ideal for in vivo observation requiring maximum space.
Learn more about the Gantry Microscope System
Inverted Microscope System — For Observing Tissue Cultures, 3D Cultures, and Cell Cultures (Spheroid)
The Inverted Frame is ideal for the time lapse observation of thick, living specimens such as tissue cultures, and three-dimensional cell cultures.as The inverted frame system also finds utility in intravital time lapse observation of organs and tissues through a body window.
Learn more about the Inverted Microscope System
One Laser System
This streamlined system uses a single multiphoton infrared laser for imaging. Add an optional SIM scanner for visible laser stimulation.
Dual Laser Lines System
This system supports dual wavelengths for multiphoton, multicolor imaging. Add an optional SIM scanner for visible laser stimulation and simultaneous multiphoton stimulation at 1045 nm.
Twin Lasers System
This flexible system employs two multiphoton infrared lasers for imaging. In addition to multiphoton, multicolor imaging, visible laser stimulation and simultaneous multiphoton stimulation across tunable wavelengths is also supported in combination with an optional SIM scanner.
Lasers for Multiphoton Configurations
The InSight X3 Dual-OL laser enables dual wavelength, simultaneous imaging for deep observation. It has a high peak power with short, 120 fs pulse widths, a broad continuously tunable range from 680 nm to 1300 nm, and a fixed wavelength line at 1045 nm. A selection of other models are available to match your multiphoton excitation requirements.
Manufacturer | Model | Wavelength covered |
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Spectra-Physics | MAITAI HPDS-OL | 690 nm - 1040 nm |
MAITAI eHPDS-OL | 690 nm - 1040 nm | |
INSIGHT X3-OL | 680 nm - 1300 nm | |
INSIGHT X3 DUAL-OL | 680 nm - 1300 nm | |
INSIGHT X3 DUALC-OL | 1045 nm (fixed) |
Manufacturer | Model | Wavelength covered |
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COHERENT | Chameleon Vision I Olympus | 690 nm - 1040 nm |
Chameleon Vision II Olympus | 680 nm - 1080 nm | |
Chameleon Vision S Olympus | 690 nm - 1050 nm |
*Chemeleon Series of lasers are not available from Olympus in some regions.
Visible Beam Combiner for Laser Light Stimulation
The laser combiner enables solid-state laser combinations for laser light stimulation at wavelengths of 405 nm, 458 nm, and 588 nm.
Learn more about the Laser Combiner
Light Guide Illumination Source U-HGLGPS
This light source is equipped with a liquid light guide that minimizes the impact of vibration and lamp heat on both the microscope and specimens. With a metal-halide bulb, the light source offers an average lifetime of 2000 hours.
Transmitted Non-descanned Light Detector
A high NA condenser and transmitted non-descanned light detector for multiphoton imaging detect fluorescence and harmonic emissions scattered in the forward direction from the sample plane.
Learn more about the Non-descanned Detector
Multialkali PMT 2CH Detector
This basic multialkali 2CH PMT provides robust performance across a wide range of wavelengths.
Learn more about the Multialkali PMT 2CH Detector
Multialkali PMT 4CH Detector
Expand your simultaneous detection capability with a total of 4 multialkali PMTs.
Learn more about the Multialkali PMT 2CH + 2CH Detector
Multialkali PMT 2CH + 2CH Cooled GaAsP PMT Detector
Combine the robustness and dynamic range of multialkali PMTs with the high sensitivity of GaAsP PMTs.
Learn more about the Cooled GaAsP PMT 2-Channel Detector
Cooled GaAsP PMT 2CH Detector
This Peltier-cooled 2CH GaAsP PMT provides higher sensitivity for weak signals or short pixel dwell times.
Laser Unit > Qualified IR Pulsed Laser with Negative Chirp for Multiphoton Excitation | <Spectra-Physics products> <Coherent products> <Main IR pulsed laser> <Additional IR line/ Laser> | |
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Laser Unit > Automatic Introduction Optic | <One Laser System> <Dual Lines, Twin Lasers System> | |
Laser Unit > IR Laser Combining Optic | <Dual Lines, Twin Lasers System> | |
Laser Unit > Optional Visible Light Laser for Stimulation | 405 nm/50 mW, 458 nm/20 mW, 588 nm/20 mW laser source with AOTF attenuation. 0% — 100%, 0.1% increment, < 2 μs rising time | |
Scanning and Detection > Main Scanner > Standard Laser Ports |
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Scanning and Detection > Main Scanner > Detector > Standard |
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Scanning and Detection > Main Scanner > Detector > Cooled GaAsP-PMT 2 CH |
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Scanning and Detection > Main Scanner > Detector > Optional 4 CH |
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Scanning and Detection > Main Scanner > Photo Detection Method > Analog Integration |
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Scanning and Detection > Galvanometer Scanner (Normal Imaging) > Galvanometer Mirror Scanner (X, Y) |
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Scanning and Detection > Galvanometer Scanner (Normal Imaging) > Scanning Modes > 2D | XY, XZ, XT, Xλ | |
Scanning and Detection > Galvanometer Scanner (Normal Imaging) > Scanning Modes > 3D | XYZ, XYT, XYλ, XZT, XTλ, XZλ | |
Scanning and Detection > Galvanometer Scanner (Normal Imaging) > Scanning Modes > 4D | XYZT, XZTλ, XYTλ | |
Scanning and Detection > Galvanometer Scanner (Normal Imaging) > Scanning Modes > 5D | XYZTλ | |
Scanning and Detection > Galvanometer Scanner (Normal Imaging) > Scanning Modes > Other | ROI scanning: rectangle clip, ellipse, polygon, free area, line, free line and point | |
Scanning and Detection > Galvanometer Scanner (Normal Imaging) > Scanning Speed |
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Scanning and Detection > Galvanometer Scanner (Normal Imaging) > Scanning Zoom |
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Scanning and Detection > Resonant Scanner (High-Speed Imaging) > Scanning Modes > 2D | XY, XZ, XT | |
Scanning and Detection > Resonant Scanner (High-Speed Imaging) > Scanning Modes > 3D | XYZ, XYT, XZT | |
Scanning and Detection > Resonant Scanner (High-Speed Imaging) > Scanning Modes > 4D | XYZT | |
Scanning and Detection > Resonant Scanner (High-Speed Imaging) > Scanning Modes > 5D | - | |
Scanning and Detection > Resonant Scanner (High-Speed Imaging) > Scanning Modes > other | ROI scanning: rectangle clip, line | |
Scanning and Detection > Resonant Scanner (High-Speed Imaging) > Scanning Speed | 30 fps at 512 x 512, 438 fps at 512 x 32. | |
Scanning and Detection > Resonant Scanner (High-Speed Imaging) > Scanning Zoom | 1.0 X - 8.0 X with 0.01 X increment | |
Scanning and Detection > Field Number (NA) |
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Scanning and Detection > Z-Drive |
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Scanning and Detection > Transmitted Light Detector Unit | Module with integrated external transmitted light photomultiplier detector and 100 W Halogen lamp, motorized switching, fiber adaptation to microscope frame | |
Microscope > Frame |
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System Control > Controller |
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System Control > Power Supply Unit | - | |
Optional Unit > SIM Scanner |
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Software > Basic Features | Dark room matching GUI design. User-arrangeable layout. | |
Software > IR Laser Controlling |
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Software > Optional Motorised-Stage Software | XY motorised-stage control, map image acquisition for easy target locating. Tiling acquisition and software image stitching. | |
Software > Optional Mapping and Multiplepoint Simulation Software | Multiple-point stimulation and data acquisition software. Mapping multiple-point stimulation to generate reaction map. Filtering feature to select points. Single or repeat stimulation. | |
Software > Optional Sequencer Manager | Advanced programmable software to define multiple imaging/ stimulation tasks and execute by hardware sequencer. | |
Software > Optional Auto Compensation Software | Auto spherical aberration compensation software. | |
Dimensions, Weight and Power Consumption > Microscope with Scan Unit > Dimensions (mm) |
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Dimensions, Weight and Power Consumption > Operating Environment (Indoor Use) > Ambient Temperature | Raumtemperatur: 20 - 25 °C | |
Dimensions, Weight and Power Consumption > Operating Environment (Indoor Use) > Maximum Relative Humidity | 75 % oder weniger bei 25 °C erfordert eine kontinuierliche (24 Stunden) Stromversorgung |
FV30-AC10SV | FV30-AC25W | |
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Magnifications | 10 | 25 |
NA | 0.6 | 1.05 |
W.D. (mm) | 8 | 2 |
Cover Glass Thickness (mm) | 0 - 0.23 | 0 - 0.23 |
Immersion Liquid | SCALEVIEW-A2 (Water, Silicone oil, and Normal oil available) | Water |
Special features | Auto Compensation, Optimized for multiphoton imaging | Auto Compensation, Optimized for multiphoton imaging |
Dimensions | 56mm (W) x 106.5mm (D) x 95mm(H) | 56mm (W) x 106.5mm (D) x 101mm(H) |
Weight | approx. 1kg | approx. 1kg |
4mm 3D Stack on Blood Vessel Label
Loading the player… FVMPE-RS: 4-mm-3D-Stapel auf Blutgefäß-Label![]() |
4mm 3D Stack on Blood Vessel Label with Texas Red in Mouse Brain
Image data courtesy of: Hiroshi Hama, Rie Ito, Atsushi Miyawaki Laboratory for Cell Function Dynamics, RIKEN Brain Science Institute |
Mouse in vivo Brain
Maximum projection of images acquired at around 600um depth acquired by using TruResolution objective with auto adjustment function
Image data courtesy of:
Dr. Hiromu Monai, Dr.Hajime Hirase and Dr. Atsushi Miyawaki
RIKEN BSI-Olympus Collaboration Center
3D Reconstructed Image of Mouse in vivo Brain
Mouse in vivo brain (Thy1-YFP-H mouse, sensory cortex) acquired by using TruResolution objective with auto adjustment function
Image data courtesy of:
Dr. Hiromu Monai, Dr.Hajime Hirase and Dr. Atsushi Miyawaki
RIKEN BSI-Olympus Collaboration Center
Copyright OLYMPUS CORPORATION, All rights reserved.
Copyright OLYMPUS CORPORATION, All rights reserved.
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