Optical Clearing with SCALEVIEW-A2

By making the tissue transparent, while simultaneously minimizing light scattering, images can be obtained from 8 mm below the surface of the sample. Through the elimination of tissue slicing, it is much easier for researchers to visualise how neural filaments connect in the brain for example; a process which is quite difficult when thin sections need to be pieced together to provide an accurate representation. As such, the SCALEVIEW objective lenses, in combination with the SCALEVIEW-A2 reagent and FLUOVIEW multiphoton microscopes, provide a perfect option for developmental biology studies, as well as imaging and mapping of the brain and other organs.
The revolutionary SCALEVIEW approach was developed in collaboration with the RIKEN Brain Institute in Japan, where it allowed researchers to create highly accurate 3D structural representations of brain tissue. As part of a complete system, the new SCALEVIEW approach integrates seamlessly with Olympus’s FLUOVIEW multiphoton microscopes, providing the power to visualise 3-dimensional structures at unprecedented depths in morphologically intact tissue.