Solutions en sciences de la vie
FV1200 Multi Point and Mapping Software
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The multi point and mapping software module for the FV1200 and FV1200MPE microscope enables the user to perform precise light stimulation of multiple points, to extend multiple points to cover a small area, or to stimulate of a rectangular area of interest for mapping scans. Stimulation timing, duration and intervals can be defined very precisely, and it is also possible to program continuous or pulse stimulation. Software control ensures that neighboring points are not excited, while synchronization and stimulation with imaging to microsecond precision allow detailed analysis of the connectivity of cells.
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The user can designate the number of points on an image for light stimulation. Stimulation timing, duration and interval can be freely defined, and the user can program the experiment with continuous or pulse stimulation. The software also provides features that allow the stimulation of extended multiple points surrounding one single point to cover a small area.
Light stimulation can be applied to a rectangular region of interest and software control ensures that neighboring points will not be excited, allowing the user to observe the reaction of the target sample more accurately. Changes in intensity from those points can be processed as a mapped image or graph. Multiple points can be automatically set to pixels exceeding a defined threshold, helping to define a multi point scan of the active regions.
The analog unit of the FV1200 enables voltages to be converted into images that can be handled just like fluorescence images. For example, electrical signals measured by patch clamping during a mapping scan can be displayed with pseudo-color and overlaid with a fluorescent image.
This unit is fully compatible with the FLUOVIEW FV1000 and FV1000MPE systems.
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