Human Cervical Adenocarcinoma Cells (HeLa Line)
A culture of HeLa carcinoma cells* was transfected with a pEGFP-Nucleus plasmid subcellular localization vector. Plasmid pEYFP-Nucleus vector gene product expression in various cell types occurs due to the efficient intracellular translation of a fusion nucleotide sequence combining the enhanced green fluorescent protein domain with three tandem repeats of the nuclear localization signal (NLS) from simian virus 40 (SV40) large T-antigen. In addition, the cells were labeled with Alexa Fluor 568 conjugated to phalloidin in order to target the cytoskeletal filamentous actin network.
*Although it became one of the most important cell lines in medical research, it’s imperative that we recognize Henrietta Lacks’ contribution to science happened without her consent. This injustice, while leading to key discoveries in immunology, infectious disease, and cancer, also raised important conversations about privacy, ethics, and consent in medicine.
To learn more about the life of Henrietta Lacks and her contribution to modern medicine, click here.
http://henriettalacksfoundation.org/
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