Evident LogoOlympus Logo

Pergunte a um especialista

Deconvolution of 3D Image Stacks

Images taken with a light microscope are never true representations of the specimen. Error sources, which have to be controlled, are sample preparation and staining protocols as well as optical aberrations and limitations of microscope and digital camera.

To turn the microscopic image into a close representation of the specimen ground truth restoration algorithms are in use. In this presentation, we will focus on restoration of three-dimensional datasets, a process called deconvolution. The mathematics behind these methods will not be covered. Rather, the aim is to provide a brief, intuitive introduction that allows us to assess the possible uses of these filters in a specific application. For this reason, we will demonstrate these filters live on selected example images.

Presenter: Heiko Gäthje
Senior Trainer, Olympus Europa

Heiko joined Olympus in 2004 as a web content manager in the Marketing and Communication team. Since 2008, he has worked as a microscopy trainer at the Olympus Academy and is responsible for the conception and introduction of digital learning tools. In addition, he supports and conducts microscopy training courses at the EMBL Heidelberg and the Zurich Winter School on Advanced Microscopy where he regularly answers questions related to image processing and image analysis.

Deconvolution of 3D Image Stacks

Images taken with a light microscope are never true representations of the specimen. Error sources, which have to be controlled, are sample preparation and staining protocols as well as optical aberrations and limitations of microscope and digital camera.

To turn the microscopic image into a close representation of the specimen ground truth restoration algorithms are in use. In this presentation, we will focus on restoration of three-dimensional datasets, a process called deconvolution. The mathematics behind these methods will not be covered. Rather, the aim is to provide a brief, intuitive introduction that allows us to assess the possible uses of these filters in a specific application. For this reason, we will demonstrate these filters live on selected example images.

Presenter: Heiko Gäthje
Senior Trainer, Olympus Europa

As a biologist with a focus on neuronal development of insects and the structure of sialic acid binding neuronal proteins in mammals, Heiko Gaethje gained first experience with widefield and confocal fluorescence microscopy and image processing of 3D data.

Heiko joined Olympus in 2004 as a web content manager in the Marketing and Communication team. Since 2008, he has worked as a microscopy trainer at the Olympus Academy and is responsible for the conception and introduction of digital learning tools. In addition, he supports and conducts microscopy training courses at the EMBL Heidelberg and the Zurich Winter School on Advanced Microscopy where he regularly answers questions related to image processing and image analysis.

Especialistas
Heiko Gäthje
Instrutor Sênior, Academia de Treinamento
Olympus Europa

Olá, meu nome é Heiko Gäthje. Minha experiência em campo amplo, microscopia de fluorescência confocal e processamento de imagem de dados 3D começou quando trabalhava como biólogo — concentrei-me no desenvolvimento neuronal de insetos e na estrutura de proteínas neuronais de ligação de ácido siálico em mamíferos.

Entrei para a Olympus em 2004 e sou instrutor de microscopia na Olympus Academy desde 2008, onde sou responsável pela concepção e introdução de ferramentas de aprendizagem digital. Também dou suporte e conduzo cursos de formação em microscopia no EMBL Heidelberg e no Curso de Inverno de Zurique sobre Microscopia Avançada, onde respondo a muitas perguntas relacionadas ao processamento e análise de imagens.

Deconvolution of 3D Image StacksDez 15 2024
Desculpe, esta página não está disponível em seu país
Inscreva-se para perguntar ao especialista

By clicking subscribe you are agreeing to our privacy policy which can be found here.

Sorry, this page is not
available in your country.

Desculpe, esta página não está disponível em seu país