Tips for 3D Spatial Analysis of High-Content Drug Screening

High content analysis during drug screening using 3D spheroids and organoids requires optimizations for speed, effective sample imaging and clear and efficient analysis. In an upcoming webinar, imaging expert Brendan Brinkman, of Olympus, will discuss live imaging of cancer organoids in 384 well plates, optimizations with optically cleared samples, and the effects for efficient high content analysis.

What will you learn?

How to acquire fluorescent high speed 384 well plate images of live cancer organoids.
Optimizations for optical clearing, refractive index matching and accuracy of object detection.
How the NoviSight™ HCA software generates True 3D models with statistics across these samples.

Presenter:

Brendan Brinkman
Life Science New Business Strategy, Global
Scientific Solutions Division Olympus Corporation

3D Cell Analysis Software

NoviSight

NoviSight 3D cell analysis software provides statistical data for spheroids and 3D objects in microplate-based experiments. Use it to quantify cell activity in 3D, easily capture rare cell events, obtain accurate cell counts, and improve detection sensitivity. NoviSight software works with a range of imaging techniques, including point-scan confocal imaging, two-photon imaging, spinning disk confocal imaging, and super resolution live cell imaging.

Fast 3D image recognition from whole structures to subcellular features
Accurate statistical analysis
Equipped with a variety of ready-to-use default assays or easily design your own

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Super Apochromat Objectives

UPLSAPO-S/UPLSAPO-W

These super apochromat objectives provide spherical and chromatic aberration compensation and high transmission from the visible to the near infrared. Using silicone oil or water immersion media, which have refractive indexes closely matching that of live cells, they achieve high-resolution imaging deep in living tissue.

Compensate for both spherical and chromatic aberrations and high transmission from the visible to the near-infrared region
Silicone oil or water immersion media help achieve high-resolution imaging deep in living tissue and reduce spherical aberration as their refractive indexes closely match that of live cells

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