Human Cervical Adenocarcinoma Cells (HeLa Line)
HeLa cervical carcinoma epithelial cells* were transfected with an EGFP vector containing the peroxisomal targeting signal 1 (PTS1) fusion protein. Stable transfectants were isolated and subsequently labeled with MitoTracker Red CMXRos before being counterstained with Hoechst 33342. The specimen was imaged using a scanning confocal microscope equipped with an argon-ion and a helium-neon laser (488 and 543 nanometer excitation wavelengths, respectively).
*Although it became one of the most important cell lines in medical research, it’s imperative that we recognize Henrietta Lacks’ contribution to science happened without her consent. This injustice, while leading to key discoveries in immunology, infectious disease, and cancer, also raised important conversations about privacy, ethics, and consent in medicine.
To learn more about the life of Henrietta Lacks and her contribution to modern medicine, click here.
http://henriettalacksfoundation.org/
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