Fluorochrome Data Tables

Our visitors will find in the following listing many of the commonly-used fluorochromes, with their respective peak excitation and emission wavelengths. The authors wish to express particular appreciation to Molecular Probes, Inc. for supplying some of this information; several other sources in the literature were also helpful.

While the authors assume responsibility for the accurate reporting of the data as published in various reliable sources, several caveats must be given. Fluorochrome dyes are environmentally sensitive and different results will be obtained with different solvents and applications. In reviewing the literature, you will frequently find somewhat different data supplied for the identical fluorochrome. There are also, in many instances, several sub-varieties of a fluorochrome.

Fluorescence Filter Cubes

Discover how variations in excitation and barrier filters allow certain wavelengths to pass.

The "Handbook of Fluorescent Probes and Research Chemicals", published by Molecular Probes, Inc. in Eugene, Oregon is a rich source of information about fluorochrome dyes. Much information about fluorochromes will also be found in sources cited in the bibliography and Web resources guide which visitors will find on the primer introductory page. See particularly the books edited by Taylor et al. and the literature published by microscope companies. Also, consult the publications of Omega Optical and Chroma Technology.

It is strongly urged that the data for a particular fluorochrome be confirmed by consulting the dye manufacturer to secure full specification of curves for excitation and emission.

Fluorochrome Peak Excitation and Emission Wavelengths
FluorochromeExcitation
Wavelength
Emission
Wavelength
Acid Fuchsin540630
Acridine Orange
(Bound to DNA)
502526
Acridine Red455-600560-680
Acridine Yellow470550
Acriflavin436520
AFA (Acriflavin
Feulgen SITSA)
355-425460
Alizarin Complexon530-560580
Alizarin Red530-560580
Allophycocyanin650661
ACMA430474
AMCA-S, AMC345445
Aminoactinomycin D555655
7-Aminoactinomycin D-AAD546647
Aminocoumarin350445
Anthroyl Stearate361-381446
Astrazon Brilliant
Red 4G
500585
Astrazon Orange R470540
Astrazon Red 6B520595
Astrazon Yellow
7 GLL
450480
Atabrine436490
Auramine460550
Aurophosphine450-490515
Aurophosphine G450580
BAO 9-(Bisamino-
phenyloxadiazole)
365395
BCECF505530
Berberine Sulphate430550
Bisbenzamide360600-610
BOBO-1, BO-PRO-1462481
Blancophor FFG Solution390470
Blancophor SV370435
Bodipy Fl503512
Bodipy TMR542574
Bodipy TR589617
BOPRO 1462481
Brilliant Sulpho-
flavin FF
430520
Calcein494517
Calcien Blue370435
Calcium Green505532
Calcium Orange549576
Calcofluor RW Solution370440
Calcofluor White440500-520
Calcofluor White
ABT Solution
380475
Calcofluor White
Standard Solution
365435
5-(and 6-)carboxy
SNARF-1 indicator
548(low pH)
576(high pH)
587(low pH)
635(high pH)
6-Carboxyrhodamine 6G525555
Cascade Blue400425
Catecholamine410470
Chinacrine450-490515
CL-NERF504(low pH)
514(high pH)
587(low pH)
540(high pH)
Coriphosphine O460575
Coumarin-Phalloidin387470
CY3.18554568
CY5.18649666
CY7710805
DANS (1-DimethylAmino-
Naphthaline-5-Sulphonic Acid)
340525
DANSA
(DiaminoNaphthyl-
Sulphonic Acid)
340-380430
Dansyl NH-CH3 in water340578
DAPI350470
DiA456590
Diamino Phenyl
Oxydiazole (DAO)
280460
Di-8-ANEPPS488605
Dimethylamino-5-
Sulphonic Acid
310-370520
DiI [DiIC18(3)]549565
DiO [DiOC18(3)]484501
Diphenyl Brilliant
Flavine 7GFF
430520
DM-NERF497(low pH)
510(high pH)
527(low pH)
536(high pH)
Dopamine340490-520
ELF-97 alcohol345530
Eosin525545
Erythrosin ITC530558
Ethidium Bromide510595
Euchrysin430540
FIF (Formaldehyde
Induced Fluorescence)
405435
Flazo Orange375-530612
Fluorescein494518
Fluorescein Iso-
thiocyanate (FITC)
490525
Fluo 3485503
FM1-43479598
Fura-2363(low [Ca2+])
335(high [Ca2+])
512(low [Ca2+])
505(high [Ca2+])
Fura Red472(low [Ca2+])
436(high [Ca2+])
657(low [Ca2+])
637(high [Ca2+])
Genacryl Brilliant
Red B
520590
Genacryl Brilliant
Yellow 10GF
430485
Genacryl Pink 3G470583
Genacryl Yellow
5GF
430475
Gloxalic Acid405460
Granular Blue355425
Haematoporphyrin530-560580
Hoechst 33258, 33342
(Bound to DNA)
352461
3-Hydroxypyrene-5,-
8,10-TriSulfonic Acid
403513
7-Hydroxy-4-
methylcoumarin
360455
5-Hydroxy-
Tryptamine (5-HT)
380-415520-530
Indo-1350405-482
Intrawhite Cf
Liquid
360430
Leucophor PAF370430
Leucophor SF380465
Leucophor WS395465
Lissamine Rhodamine
B200 (RD200)
575595
Lucifer Yellow CH425528
Lucifer Yellow VS430535
LysoSensor Blue
DND-192, DND-167
374425
LysoSensor Green
DND-153, DND-189
442505
LysoSensor Yellow/Blue384(low pH)
329(high pH)
540(low pH)
440(high pH)
LysoTracker Green504511
LysoTracker Yellow534551
LysoTracker Red577592
Magdala Red524600
Magnesium Green506531
Magnesium Orange550575
Maxilon Brilliant
Flavin 10 GFF
450495
Maxilon Brilliant
Flavin 8 GFF
460495
Mitotracker Green FM490516
Mitotracker Orange
CMTMRos
551576
MPS (Methyl Green
Pyronine Stilbene)
364395
Mithramycin450570
NBD465535
NBD Amine450530
Nile Red515-530525-605
Nitrobenzoxadidole460-470510-650
Noradrenaline340490-520
Nuclear Fast Red289-530580
Nuclear Yellow365495
Nylosan Brilliant
Flavin E8G
460510
Oregon Green 488
fluorophore
496524
Oregon Green 500
fluorophore
503522
Oregon Green 514
fluorophore
511530
Pararosaniline
(Feulgen)
570625
Phorwite AR
Solution
360430
Phorwite BKL370430
Phorwite Rev380430
Phorwite RPA375430
Phosphine 3R465565
Phosphine R480-565578
Pontochrome
Blue Black
535-553605
POPO-1, PO-PRO-1434456
Primuline410550
Procion Yellow470600
Propidium Iodide536617
Pyronine410540
Pyronine B540-590560-650
Pyrozal Brilliant
Flavin 7GF
365495
Quinacrine
Mustard
423503
R-phycoerythrin565575
Rhodamine 110496520
Rhodamine 123511534
Rhodamine 5 GLD470565
Rhodamine 6G526555
Rhodamine B540625
Rhodamine B 200523-557595
Rhodamine B Extra550605
Rhodamine BB540580
Rhodamine BG540572
Rhodamine Green
fluorophore
502527
Rhodamine Red570590
Rhodamine WT530555
Rhodol Green fluorophore499525
Rose Bengal540550-600
Serotonin365520-540
Sevron Brilliant
Red 2B
520595
Sevron Brilliant
Red 4G
500583
Sevron Brilliant
Red B
530590
Sevron Orange440530
Sevron Yellow L430490
SITS (Primuline)395-425450
SITS (Stilbene
Isothiosulphonic Acid)
365460
Sodium Green507535
Stilbene335440
Snarf 1563639
Sulpho Rhodamine
B Can C
520595
Sulpho Rhodamine
G Extra
470570
SYTOX Green nucleic
acid stain
504523
SYTO Green fluorescent
nucleic acid stains
494 ± 6515 ± 7
SYTO Green fluorescent
nucleic acid stains
515 ± 7543 ± 13
SYTO 17 red fluorescent
nucleic acid stain
621634
Tetracycline390560
TRITC (Tetramethyl
Rhodamine Isothiocyanate)
557576
Texas Red596615
Thiazine Red R510580
Thioflavin S430550
Thioflavin TCN350460
Thioflavin 5430550
Thiolyte370-385477-484
Thiozol Orange453480
Tinopol CBS390430
TOTO 1, TO-RRO-1514533
TOTO 3, TO-PRO-3642661
True Blue365420-430
Ultralite656678
Uranine B420520
Uvitex SFC365435
X-Rhodamine580605
Xylene Orange546580
XRITC582601
YOYO-1, YOYO-PRO-1491509
YOYO-3, YOYO-PRO-3612631

Contributing Authors

Mortimer Abramowitz - Olympus America, Inc., Two Corporate Center Drive., Melville, New York, 11747.

Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.

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